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Literature summary for 7.4.2.8 extracted from
- Enzymatic characterization of the enteropathogenic Escherichia coli type III secretion ATPase EscN (2007), Arch. Biochem. Biophys., 468, 121-127.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pET19b vector for expression in Escherichia coli BL21DE3 pLysS cells | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive inhibitor | Escherichia coli |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | Escherichia coli | |
| Mn2+ | Mg2+ can be substituted by Mn2+, although the reaction rate is reduced by 50% | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
determined by SDS-PAGE, HisEscN | Escherichia coli |
| 51800 | - |
deduced molecular mass | Escherichia coli |
| 62000 | - |
monomer, determined by blue native electrophoresis | Escherichia coli |
| 327000 | - |
hexamer, determined by blue native electrophoresis | Escherichia coli |
| 622000 | - |
dodecamer, determined by blue native electrophoresis | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Ni-NTA agarose column | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | different oligomeric forms are identified by blue native electrophoresis, including a hexamer and a dodecamer | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EscN | - |
Escherichia coli |
| type III secretion ATPase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
ATPase activity assay | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 42 | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
ATP | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
ATPase activity assay | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.06 | - |
ADP | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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