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Literature summary for 7.4.2.3 extracted from

  • Latijnhouwers, M.; Xu, X.M.; Moller, S.G.
    Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development (2010), Planta, 232, 567-578.
    View publication on PubMed
Search for more literature for 7.4.2.3

Cloned(Commentary)

Cloned (Comment) Organism
cpHsc70-1 and cpHsc70-2 cDNAs are PCR amplified and cloned into NdeI and XhoI sites of pET28a and introduced into Escherichia coli BL21(DE3) Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information a T-DNA insertion line for cpHsc70-1 DELTAcpHsc70-1 has variegated cotyledons, malformed leaves, growth retardation, impaired root growth and sensitivity to heat shock treatment. In addition, under stress conditions, the mutant exhibits unusual sepals, and malformed flowers Arabidopsis thaliana
additional information cpHsc70-1/cpHsc70-2 double mutants are lethal Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast cpHsc70-1 and cpHsc70-2 are highly conserved proteins, imported into chloroplasts, analyzed by fluorescence microscopy Arabidopsis thaliana 9507
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chloroplast stroma YFP-fusion proteins of cpHsc70-1 and cpHsc70-2 are predominantly stromal, analyzed by fluorescence microscopy Arabidopsis thaliana 9570
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thylakoid membrane low level, analyzed by fluorescence microscopy Arabidopsis thaliana 42651
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Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
cpHsc70-1 highly conserved, imported into chloroplasts Arabidopsis thaliana
cpHsc70-2 highly conserved, imported into chloroplasts Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana expression patterns for cpHsc70-1 and cpHsc70-2 genes analyzed using the GUS (beta-gucuronidase) reporter gene. High Expression determined throughout seedlings, in all tissues of mature plants, unaltered in the drak or low light. Both genes are ubiquitously expressed at high levels, cpHsc70-1 slightly higher than cpHsc70-2, especially in roots additional information