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Literature summary for 7.4.2.14 extracted from
- Binding and transport of melanoma-specific antigenic peptides by the transporter associated with antigen processing (1996), Mol. Immunol., 33, 1165-1169 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AARAVFLAL | - |
Homo sapiens | |
| AYGLDFYIL | - |
Homo sapiens | |
| EADPTGHSY | - |
Homo sapiens | |
| EVDPIGHLY | - |
Homo sapiens | |
| LLDGTATLRL | - |
Homo sapiens | |
| MLLAVLYCL | - |
Homo sapiens | |
| QVPLRPMTYK | - |
Homo sapiens | |
| SAYGEPRKL | - |
Homo sapiens | |
| YLEPGPVTA | - |
Homo sapiens | |
| YMDGTMSQV | - |
Homo sapiens | |
| YMNGTMSQV | - |
Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | - |
Homo sapiens | 5789 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + antigen peptide[side 1] | Homo sapiens | - |
ADP + phosphate + antigen peptide[side 2] | - |
? | |
| additional information | Homo sapiens | some melanoma-specific epitopes are good substrates for TAP, while others are poor substrates for TAP. One of the epitopes derived from tyrosinase is transported into the endoplasmic reticulum (ER) in spite of being a poor competitor for reporter peptide transport and for peptide binding. Transport of melanoma epitopes which posess N-linked glycosylation sites, substrate specificity, overview | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q03518 AND Q03519 | TAP1 and TAP2 subunits | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| melanoma cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + AARAVFLAL[side 1] | - |
Homo sapiens | ADP + phosphate + AARAVFLAL[side 2] | - |
? | |
| ATP + H2O + antigen peptide[side 1] | - |
Homo sapiens | ADP + phosphate + antigen peptide[side 2] | - |
? | |
| ATP + H2O + AYGLDFYIL[side 1] | - |
Homo sapiens | ADP + phosphate + AYGLDFYIL[side 2] | - |
? | |
| ATP + H2O + EADPTGHSY[side 1] | - |
Homo sapiens | ADP + phosphate + EADPTGHSY[side 2] | - |
? | |
| ATP + H2O + EVDPIGHLY[side 1] | - |
Homo sapiens | ADP + phosphate + EVDPIGHLY[side 2] | - |
? | |
| ATP + H2O + LLDGTATLRL[side 1] | - |
Homo sapiens | ADP + phosphate + LLDGTATLRL[side 2] | - |
? | |
| ATP + H2O + MLLAVLYCL[side 1] | - |
Homo sapiens | ADP + phosphate + MLLAVLYCL[side 2] | - |
? | |
| ATP + H2O + QVPLRPMTYK[side 1] | - |
Homo sapiens | ADP + phosphate + QVPLRPMTYK[side 2] | - |
? | |
| ATP + H2O + SAYGEPRKL[side 1] | - |
Homo sapiens | ADP + phosphate + SAYGEPRKL[side 2] | - |
? | |
| ATP + H2O + YLEPGPVTA[side 1] | - |
Homo sapiens | ADP + phosphate + YLEPGPVTA[side 2] | - |
? | |
| ATP + H2O + YMDGTMSQV[side 1] | - |
Homo sapiens | ADP + phosphate + YMDGTMSQV[side 2] | - |
? | |
| ATP + H2O + YMNGTMSQV[side 1] | - |
Homo sapiens | ADP + phosphate + YMNGTMSQV[side 2] | - |
? | |
| additional information | some melanoma-specific epitopes are good substrates for TAP, while others are poor substrates for TAP. One of the epitopes derived from tyrosinase is transported into the endoplasmic reticulum (ER) in spite of being a poor competitor for reporter peptide transport and for peptide binding. Transport of melanoma epitopes which posess N-linked glycosylation sites, substrate specificity, overview | Homo sapiens | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TAP | - |
Homo sapiens |
| TAP1 | - |
Homo sapiens |
| TAP2 | - |
Homo sapiens |
| transporter associated with antigen processing | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
pH 7.3, 37°C, versus labeld QVPLRPMTYK-HSAB peptide | Homo sapiens | AARAVFLAL | |
| 0.0012 | - |
pH 7.3, 37°C, versus labeld QVPLRPMTYK-HSAB peptide | Homo sapiens | QVPLRPMTYK | |
| 0.0019 | - |
pH 7.3, 37°C, versus labeld QVPLRPMTYK-HSAB peptide | Homo sapiens | LLDGTATLRL | |
| 0.004 | - |
pH 7.3, 37°C, versus labeld QVPLRPMTYK-HSAB peptide | Homo sapiens | SAYGEPRKL | |
| 0.009 | - |
pH 7.3, 37°C, versus labeld QVPLRPMTYK-HSAB peptide | Homo sapiens | AYGLDFYIL | |
| 0.025 | - |
pH 7.3, 37°C, versus labeld QVPLRPMTYK-HSAB peptide | Homo sapiens | YMNGTMSQV | |
| 0.078 | - |
pH 7.3, 37°C, versus labeld QVPLRPMTYK-HSAB peptide | Homo sapiens | YLEPGPVTA | |
| 0.08 | - |
pH 7.3, 37°C, versus labeld QVPLRPMTYK-HSAB peptide | Homo sapiens | EADPTGHSY | |
| 0.1 | - |
above, pH 7.3, 37°C, versus labeld QVPLRPMTYK-HSAB peptide | Homo sapiens | MLLAVLYCL | |
| 0.1 | - |
above, pH 7.3, 37°C, versus labeld QVPLRPMTYK-HSAB peptide | Homo sapiens | YMDGTMSQV | |
| 0.1 | - |
above, pH 7.3, 37°C, versus labeld QVPLRPMTYK-HSAB peptide | Homo sapiens | EVDPIGHLY |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | binding and transport of melanoma-specific antigenic peptides by the transporter associated with antigen processing. The melanoma epitopes exhibit differential capacities to be transported by TAP in streptolysin O-permeabilized cells, as well as differential competition for peptide binding to TAP. The melanoma antigens follow distinct pathways for presentation, along the MHC class I pathway. The epitopes YMNGTMSQV and YMDGTMSQV are two forms of the same epitope derived from tyrosinase. The D form of the epitope is thought to arise from the conversion of asparagine to aspartic acid during enzymatic deglycosylation of the epitope. The competition by melanoma epitopes observed in permeabilized cells also occurs at the level of peptide binding to TAP, overview | Homo sapiens |
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