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Literature summary for 7.4.2.14 extracted from
- Residues in TAP2 peptide transporters controlling substrate specificity (1996), J. Immunol., 156, 1756-1763 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes TAP1 and TAP2, recombinant expression of chimeric rTAP2 molecules together with rTAPl in human TAP-deficient T2 cells. All hybrids are functional, the surface expression of HLA-B51 molecules on T2 cells is induced to comparable extents, indicating TAP-dependent peptide transport and class I loading. Peptide transport by T2 cells expressing wild-type rTAP2a, rTAP2u, or rTAP2 hybrids is analyzed | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mapping of two short stretches in rat TAP2, with two polymorphic residues each, that essentially control the differential peptide transport observed for the rat alleles by constructing several hybrids between rat TAP2a and TAP2u and co-expressing them wmith rat TAPl in TAP-deficient T2 cells. The critical residues are located in putative cytoplasmic loops close to the membrane. Relation of diverse polymorphic residues TAP2 controlling substrate specificity to the membrane topology, overview | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | - |
Rattus norvegicus | 5789 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Rattus norvegicus |  |
| Mg2+ | required | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + antigen peptide[side 1] | Rattus norvegicus | - |
ADP + phosphate + antigen peptide[side 2] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P36370 AND P36372 | subunits TAP1 and TAP2 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + antigen peptide[side 1] | - |
Rattus norvegicus | ADP + phosphate + antigen peptide[side 2] | - |
? | |
| additional information | residues in TAP2 peptide transporters control the substrate specificity, overview. Iodinated peptides of the RYWANATRSX and TVDNKTRYX with X = different amino acids are used for peptide transport assays, overview | Rattus norvegicus | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TAP | - |
Rattus norvegicus |
| TAP1 | - |
Rattus norvegicus |
| TAP2 | - |
Rattus norvegicus |
| transporter associated with Ag processing | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rattus norvegicus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | mapping of two short stretches in rat TAP2, with two polymorphic residues each, that essentially control the differential peptide transport observed for the rat alleles by constructing several hybrids between rat TAP2a and TAP2u and coexpressing them wmith rat TAPl in TAP-deficient T2 cells | Rattus norvegicus |
| physiological function | the transporter associated with Ag processing (TAP) translocates peptides from the cytosol into the endoplasmic reticulum lumen where they associate with MHC class I molecules | Rattus norvegicus |
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