Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | chloroplastic isoform GS2 | Secale cereale x Triticum aestivum | 9507 | - |
cytoplasm | cytoplasmic isoform GS1 | Secale cereale x Triticum aestivum | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mg2+ in activation | Secale cereale x Triticum aestivum | |
Mg2+ | divalent metal ion required, Mg2+ is most effective. Maximal activation of GS1 at 5 mM and of GS2 at 20 mM | Secale cereale x Triticum aestivum | |
Mn2+ | can partially replace Mg2+ in activation | Secale cereale x Triticum aestivum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
8 * 38000, isoform GS1, SDS-PAGE | Secale cereale x Triticum aestivum |
48000 | - |
8 * 48000, isoform GS2, SDS-PAGE | Secale cereale x Triticum aestivum |
305000 | - |
isoform GS1, gel filtration | Secale cereale x Triticum aestivum |
385200 | - |
isoform GS2, gel filtration | Secale cereale x Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Secale cereale x Triticum aestivum | - |
variety Malno | - |
Purification (Comment) | Organism |
---|---|
cytoplasmic isoform GS1 and chloroplastic isoform GS2 | Secale cereale x Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | of seedlings | Secale cereale x Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.5 | - |
chloroplastic isoform GS2 | Secale cereale x Triticum aestivum |
35.1 | - |
cytoplasmic isoform GS1 | Secale cereale x Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Secale cereale x Triticum aestivum | ADP + phosphate + L-Gln | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 48000, isoform GS2, SDS-PAGE | Secale cereale x Triticum aestivum |
octamer | 8 * 38000, isoform GS1, SDS-PAGE | Secale cereale x Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
isoform GS1, Mn2+-activated enzyme | Secale cereale x Triticum aestivum |
5.4 | - |
isoform GS2, Mn2+-activated enzyme | Secale cereale x Triticum aestivum |
6.8 | - |
isoform GS1, Co2+-activated enzyme | Secale cereale x Triticum aestivum |
7 | - |
isoform GS2, Co2+-activated enzyme | Secale cereale x Triticum aestivum |
7.7 | - |
isoform GS1, Mg2+-activated enzyme | Secale cereale x Triticum aestivum |
8.2 | - |
isoform GS2, Mg2+-activated enzyme | Secale cereale x Triticum aestivum |