BRENDA - Enzyme Database show
show all sequences of 6.2.1.8

The oxalyl-CoA synthetase-regulated oxalate and its distinct effects on resistance to bacterial blight and aluminium toxicity in rice

Peng, C.; Liang, X.; Liu, E.E.; Zhang, J.J.; Peng, X.X.; Plant Biol. 19, 345-353 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Oryza sativa
Engineering
Amino acid exchange
Commentary
Organism
C219G
the mutant shows 5.6% activity compared to the wild type enzyme
Oryza sativa
C287G
the mutant shows 25.4% activity compared to the wild type enzyme
Oryza sativa
C363G
the mutant shows 7.2% activity compared to the wild type enzyme
Oryza sativa
C454G
the mutant shows 44.5% activity compared to the wild type enzyme
Oryza sativa
C473G
the mutant shows 42.9% activity compared to the wild type enzyme
Oryza sativa
Inhibitors
Inhibitors
Commentary
Organism
Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
almost complete inhibition at 5 mM
Oryza sativa
5,5'-dithiobis-(2-nitrobenzoic acid)
less than 10% residual activity at 0.1 mM
Oryza sativa
N-ethylmaleimide
complete inhibition in Tris-HCl at 0.1 mM
Oryza sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.73
-
oxalate
wild type enzyme, at pH 7.5 and 37°C
Oryza sativa
2.62
-
oxalate
mutant enzyme C473G, at pH 7.5 and 37°C
Oryza sativa
3.82
-
oxalate
mutant enzyme C219G, at pH 7.5 and 37°C
Oryza sativa
4.64
-
oxalate
mutant enzyme C363G, at pH 7.5 and 37°C
Oryza sativa
44.5
-
oxalate
mutant enzyme C454G, at pH 7.5 and 37°C
Oryza sativa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryza sativa
5829
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + oxalate + CoA
Oryza sativa
the enzyme is highly specific for oxalate
AMP + diphosphate + oxalyl-CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa
-
cultivar Zhonghua 11
-
Purification (Commentary)
Commentary
Organism
nickel chelating affinity column chromatography
Oryza sativa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf epidermal cell
-
Oryza sativa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + oxalate + CoA
the enzyme is highly specific for oxalate
746008
Oryza sativa
AMP + diphosphate + oxalyl-CoA
-
-
-
?
additional information
no activity with malonate, succinate, malate, acetate, formate, lactate, glycolate, and glyoxylate
746008
Oryza sativa
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Oryza sativa
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Oryza sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Oryza sativa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Oryza sativa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C219G
the mutant shows 5.6% activity compared to the wild type enzyme
Oryza sativa
C287G
the mutant shows 25.4% activity compared to the wild type enzyme
Oryza sativa
C363G
the mutant shows 7.2% activity compared to the wild type enzyme
Oryza sativa
C454G
the mutant shows 44.5% activity compared to the wild type enzyme
Oryza sativa
C473G
the mutant shows 42.9% activity compared to the wild type enzyme
Oryza sativa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
almost complete inhibition at 5 mM
Oryza sativa
5,5'-dithiobis-(2-nitrobenzoic acid)
less than 10% residual activity at 0.1 mM
Oryza sativa
N-ethylmaleimide
complete inhibition in Tris-HCl at 0.1 mM
Oryza sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.73
-
oxalate
wild type enzyme, at pH 7.5 and 37°C
Oryza sativa
2.62
-
oxalate
mutant enzyme C473G, at pH 7.5 and 37°C
Oryza sativa
3.82
-
oxalate
mutant enzyme C219G, at pH 7.5 and 37°C
Oryza sativa
4.64
-
oxalate
mutant enzyme C363G, at pH 7.5 and 37°C
Oryza sativa
44.5
-
oxalate
mutant enzyme C454G, at pH 7.5 and 37°C
Oryza sativa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryza sativa
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + oxalate + CoA
Oryza sativa
the enzyme is highly specific for oxalate
AMP + diphosphate + oxalyl-CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
nickel chelating affinity column chromatography
Oryza sativa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf epidermal cell
-
Oryza sativa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + oxalate + CoA
the enzyme is highly specific for oxalate
746008
Oryza sativa
AMP + diphosphate + oxalyl-CoA
-
-
-
?
additional information
no activity with malonate, succinate, malate, acetate, formate, lactate, glycolate, and glyoxylate
746008
Oryza sativa
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Oryza sativa
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in oxalate catabolism
Oryza sativa
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in oxalate catabolism
Oryza sativa
Other publictions for EC 6.2.1.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746008
Peng
The oxalyl-CoA synthetase-reg ...
Oryza sativa
Plant Biol.
19
345-353
2017
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1
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5
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3
5
1
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4
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1
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2
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1
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1
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1
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5
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5
1
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1
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1
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1
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2
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1
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1
1
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745810
Fan
Crystal structures of Arabido ...
Arabidopsis thaliana
Mol. Plant
9
1349-1352
2016
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1
5
-
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1
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1
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1
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2
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1
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1
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1
1
5
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1
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1
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1
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1
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1
1
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-
746118
Lou
An oxalyl-CoA synthetase is i ...
Vigna umbellata
Plant Physiol.
172
1679-1690
2016
-
-
-
-
-
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1
3
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1
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3
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1
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1
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1
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1
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1
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1
3
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1
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1
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2
1
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-
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1
1
1
1
-
-
746200
Foster
An oxalyl-CoA dependent pathw ...
Medicago truncatula
PLoS ONE
11
e0149850
2016
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1
-
-
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1
1
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1
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4
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1
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1
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1
1
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1
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1
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1
1
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1
1
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1
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1
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1
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1
1
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1
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1
1
-
-
-
727531
Foster
An oxalyl-CoA synthetase is im ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
FEBS Lett.
588
160-166
2014
-
-
1
-
-
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2
-
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2
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3
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1
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2
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1
1
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1
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1
1
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2
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2
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1
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2
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1
1
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1
2
2
1
-
-
728474
Foster
A previously unknown oxalyl-Co ...
Arabidopsis thaliana
Plant Cell
24
1217-1229
2012
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1
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1
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1
1
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1
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4
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1
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3
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1
1
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1
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1
1
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1
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1
1
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1
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1
-
-
3
-
-
-
-
-
1
1
-
-
-
3
3
-
-
-
846
Sehgal
-
Purification of oxalyl CoA syn ...
Lathyrus sativus, Lathyrus sativus P-24
J. Plant Biochem. Biotechnol.
1
97-100
1992
-
-
-
-
-
-
-
-
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1
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2
-
2
-
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1
-
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1
1
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4
-
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1
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1
1
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4
-
-
-
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-
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-
-
-
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-
-
-
-
845
Adsule
Occurrence of oxalyl-CoA synth ...
Cajanus indicus, Cicer arietinum, Glycine max, Lathyrus sativus, Lens sp., Pisum sativum, Vigna radiata var. radiata, Vigna unguiculata subsp. unguiculata
Experientia
33
416-417
1977
-
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9
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8
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8
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
847
Giovanelli
Oxalyl-coenzyme A synthetase f ...
Lupinus sp., Pisum sativum, Pumpkin, Triticum aestivum
Biochim. Biophys. Acta
118
124-143
1966
-
-
-
-
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5
3
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1
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4
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4
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5
-
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1
1
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3
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1
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4
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5
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1
1
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