Information on EC 6.2.1.8 - oxalate-CoA ligase

Substrates: no activity with malonate, succinate, malate, acetate, formate, lactate, glycolate, and glyoxylate
Products: -

additional information

Substrates: no activity with malonate, succinate, malate, formate, acetate, lactate, glycolate, and glutarate
Products: -

additional information

Substrates: no activity with malonate, succinate, malate, formate, acetate, lactate, glycolate, and glutarate
Products: -

show all columns Go to Natural Substrate Search

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

ATP + oxalate + CoA

ATP + oxalate + CoA

AMP + diphosphate + oxalyl-CoA

10 entries

ATP + oxalate + CoA

Substrates: key enzyme in the biosynthesis of beta-oxalyl amino alanine
Products: -

ATP + oxalate + CoA

Lathyrus sativus P-24

Substrates: key enzyme in the biosynthesis of beta-oxalyl amino alanine
Products: -

ATP + oxalate + CoA

AMP + diphosphate + oxalyl-CoA

Substrates: -
Products: -

ATP + oxalate + CoA

AMP + diphosphate + oxalyl-CoA

Substrates: oxalate is the specific substrate
Products: -

ATP + oxalate + CoA

AMP + diphosphate + oxalyl-CoA

Substrates: -
Products: -

ATP + oxalate + CoA

AMP + diphosphate + oxalyl-CoA

Substrates: -
Products: -

ATP + oxalate + CoA

AMP + diphosphate + oxalyl-CoA

Substrates: -
Products: -

ATP + oxalate + CoA

AMP + diphosphate + oxalyl-CoA

Substrates: the enzyme is highly specific for oxalate
Products: -

ATP + oxalate + CoA

AMP + diphosphate + oxalyl-CoA

Substrates: -
Products: -

ATP + oxalate + CoA

AMP + diphosphate + oxalyl-CoA

Substrates: -
Products: -

ATP + oxalate + CoA

AMP + diphosphate + oxalyl-CoA

Substrates: -
Products: -

ATP + oxalate + CoA

AMP + diphosphate + oxalyl-CoA

Substrates: oxalate is the specific substrate
Products: -

show all columns Go to Cofactor Search

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

ATP

7 entries

ATP

show all columns Go to Metals and Ions Search

ATP

ATP

ATP

ATP

ATP

ATP

dependent on

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Mg2+

4 entries

Mg2+

required in form of Mg-ATP

Mg2+

required

Mg2+

required

Mg2+

required, 5 mM used in assay conditions

show all columns Go to Inhibitor Search

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1-ethyl-3-(3-dimethylaminopropyl)carbodiimide

almost complete inhibition at 5 mM

5,5'-dithiobis-(2-nitrobenzoic acid)

less than 10% residual activity at 0.1 mM

hydroxylamine

inhibition of ATP-diphosphate exchange

MgCl2

high concentrations cause a marked inhibition of ATP-diphosphate exchange

N-ethylmaleimide

complete inhibition in Tris-HCl at 0.1 mM

NaF

slight inhibition of ATP-diphosphate exchange

oxalate

high concentrations cause a slight inhibition of ATP-diphosphate exchange

PCMB

inhibition of ATP-diphosphate exchange. Reversal of inhibition by GSH or cysteine

show all columns Go to KM Value Search

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

ATP

0.1

CoA

0.02 - 44.5

oxalate

14 entries

additional information

0.02

oxalate

pH 7.5, temperature not specified in the publication

0.04215

oxalate

pH and temperature not specified in the publication

0.0715

oxalate

at pH 8.0 and 25°C

0.081

oxalate

at pH 8.0, temperature not specified in the publication

0.1051

oxalate

at pH 8.0, temperature not specified in the publication

0.121

oxalate

at pH 4.5 and 25°C

0.149

oxalate

pH 8.0, temperature not specified in the publication

0.2238

oxalate

isoform AAE3-1, at pH 8.0, temperature not specified in the publication

1.73

oxalate

wild type enzyme, at pH 7.5 and 37°C

oxalate

2.62

oxalate

mutant enzyme C473G, at pH 7.5 and 37°C

3.82

oxalate

mutant enzyme C219G, at pH 7.5 and 37°C

4.64

oxalate

mutant enzyme C363G, at pH 7.5 and 37°C

44.5

oxalate

mutant enzyme C454G, at pH 7.5 and 37°C

additional information

Michaelis-Menten kinetics

additional information

Michaelis-Menten kinetics

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

7.6

oxalate

at pH 8.0 and 25°C

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

110

oxalate

at pH 8.0 and 25°C

show all columns Go to Specific Activity Search

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

8.2

at pH 8.0 and 25°C

additional information

show all columns Go to pH Optimum Search

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

7.4

ATP-diphosphate exchange

7.5

7.5

7.5

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pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

5 - 10

activity range, profile overview

6 - 10

activity range, profile overview

6.3 - 8.6

6.3: about 60% of maximal activity, 8.6: about 50% of maximal activity, ATP-diphosphate exchange

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

728474, 745810

UniProt

brenda

Cajanus indicus

brenda

Cicer arietinum

chick-pea

brenda

Glycine max

brenda

line BW69

brenda

Lathyrus sativus P-24

brenda

Lens sp.

lentil

brenda

Lupinus sp.

brenda

ecotype R108

brenda

cultivar Zhonghua 11

brenda

Pumpkin

brenda

gene YBR222C

UniProt

brenda

gene YBR222C

UniProt

brenda

cultivar Micro-Tom

brenda

Triticum aestivum

Vigna radiata var. radiata

brenda

brenda

Vigna unguiculata subsp. unguiculata

UniProt

brenda

brenda

brenda

L. var. Telephone

brenda

845, 846

brenda

grass pea

UniProt

brenda

show all columns Go to Source Tissue Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

brenda

brenda

pod

brenda

brenda

highest expression

brenda

brenda

brenda

brenda

brenda

brenda

brenda

brenda

brenda

root tip

brenda

lowest expression

brenda

brenda

seed

Lupinus sp.

brenda

seed

Pumpkin

brenda

seed

Triticum aestivum

brenda

Cajanus indicus

brenda

Cicer arietinum

brenda

Glycine max

brenda

brenda

brenda

three days old

brenda

Lathyrus sativus P-24

three days old

brenda

Lens sp.

brenda

Vigna radiata var. radiata

brenda

Vigna unguiculata subsp. unguiculata

show all columns Go to Localization Search

brenda

brenda

brenda

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

brenda

brenda

brenda

brenda

brenda

brenda

brenda

brenda

brenda

brenda

brenda

brenda

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

malfunction

5 entries

metabolism

physiological function

6 entries

malfunction

a Scaae3 deletion mutant strain shows highly reduced oxalate degradation activity decreasing recovery from exposure to oxalate, e.g. by exposure to oxalate-secreting microbes, and to oxidative stress, e.g. by H2O2

malfunction

aae3 null mutants show increased soluble and insoluble oxalate contents compared to the wild-type enzyme, loss of AAE3 interferes with seed development affecting the integrity and function of the seed coat, phenotype, overview

malfunction

reduction of enzyme expression increases susceptibility of Medicago truncatula to the oxalate-secreting fungal pathogen Sclerotinia sclerotiorum and results in the accumulation of druse crystals of calcium oxalate

malfunction

enzyme knockout of isoform AAE3-1 improves tomato fruit quality, resulting in higher total sugar content and lower total acid content

malfunction

metabolism

the enzyme catalyzes the first step in oxalate degradation

metabolism

the enzyme is involved in oxalate catabolism

physiological function

the enzyme catalyzes the first step in a pathway of oxalate degradation to protect the cell against the harmful effects of oxalate derived from an endogenous process or an environmental source

physiological function

oxalyl-CoA synthetase is required for oxalate degradation, for normal seed development, and for defense against an oxalate-producing fungal pathogen, e.g. the fungus Sclerotinia sclerotiorum

physiological function

the enzyme is involved in aluminum tolerance

physiological function

enzyme-dependent degradation of oxalate is important for normal seed development and for defense against oxalate-producing fungal pathogens

physiological function

enzyme overexpression increases cadmium and aluminium tolerances in Arabidopsis thaliana and soybean hairy roots, which is associated with a decrease in oxalate accumulation

physiological function

the enzyme catalyzes the first step in a pathway of oxalate degradation to protect the cell against the harmful effects of oxalate derived from an endogenous process or an environmental source

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

Show AA Sequence and Transmembrane Helices (348 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.

Go to PDB and Structure Links Search

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PDB

SCOP

CATH

UNIPROT

ORGANISM

3 entries

3 entries

show all columns Go to Molecular Weight Search

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

56000

gel filtration

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

monomer

1 * 56000, SDS-PAGE

x * 56000, SDS-PAGE

x * 50000, SDS-PAGE

x * 65000, SDS-PAGE

Go to Crystallization (commentary) Search

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

apo, ATP-bound, as well as oxalate- and AMP-bound enzyme states, hanging drop vapor diffusion method, using

show all columns Go to Protein Variants Search

enzyme in complex with AMP, hanging drop vapor diffusion method, using 0.2 M NaCl and 25% (w/v) polyethylene glycol 3350 in 100 mM Tris pH 8.5

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

H214A

the mutant shows strongly reduced activity compared to the wild type enzyme

H319A

the mutant shows strongly reduced activity compared to the wild type enzyme

K500A

the mutant shows strongly reduced activity compared to the wild type enzyme

R409A

the mutant shows strongly reduced activity compared to the wild type enzyme

S289A

the mutant shows strongly reduced activity compared to the wild type enzyme

C219G

the mutant shows 5.6% activity compared to the wild type enzyme

C287G

the mutant shows 25.4% activity compared to the wild type enzyme

C363G

the mutant shows 7.2% activity compared to the wild type enzyme

C454G

the mutant shows 44.5% activity compared to the wild type enzyme

C473G

the mutant shows 42.9% activity compared to the wild type enzyme

additional information

additional information

aae3-1 and aae3-2 are null mutants are deficient in oxalyl-CoA synthetase

additional information

aae3-1 and aae3-2 are null mutants are deficient in oxalyl-CoA synthetase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

43.9

melting temperature of the apo enzyme

Go to Purification (commentary) Search

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

3 enzyme forms: OCS-1, OCS-2 and OCS-3

HisTrap column chromatography

Ni-NTA column chromatography

Ni-NTA column chromatography and Sephadex G-25 gel filtration

Ni-NTA His-Bind resin column chromatography

nickel chelating affinity column chromatography

recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography to over 90% purity

Ni-NTA column chromatography

Ni-NTA column chromatography

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli BL21 cells, tobacco leaves, and Arabidopsis thaliana

expressed in Escherichia coli BL21(DE3) cells

3 entries

expressed in Escherichia coli BLR(DE3) cells and Nicotiana tabacum leaves

expression of GFP-tagged enzyme in onion epidermal cell cytoplasm using the constitutive 35S promoter, and from binary construct, transfected into Agrobacterium tumefaciens, GFP-tagged enzyme is transiently expressed in Nicotiana benthamiana leaf cytoplasm

gene YBR222C, expression of His-tagged enzyme in Escherichia coli strain BLR(DE3)

expressed in Escherichia coli BL21(DE3) cells

expressed in Escherichia coli BL21(DE3) cells

expressed in Escherichia coli BL21(DE3) cells

Go to Expression Search

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EXPRESSION

ORGANISM

UNIPROT

LITERATURE

enzyme expression increases during fruit ripening

exogenous application of oxalate results in enzyme expression induction. Enzyme expression in rice bean root tips is enhanced greatly by aluminium stress (0.025-0.05 mM)

oxalate induced the enzyme, quantitative RT-PCR reveals that ScAAE3 transcript levels are induced within min of exposure peaking at about 1 h before declining back to steady state levels

the enzyme expression is increased in response to Cd2+ and Al3+ stresses (0.03 mM each, 4 h) by 31.83 and 20.21fold, respectively

oxalate induced the enzyme, quantitative RT-PCR reveals that ScAAE3 transcript levels are induced within min of exposure peaking at about 1 h before declining back to steady state levels

oxalate induced the enzyme, quantitative RT-PCR reveals that ScAAE3 transcript levels are induced within min of exposure peaking at about 1 h before declining back to steady state levels

top print hide References Search

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

Adsule, R.N.; Barat, G.K.

Occurrence of oxalyl-CoA synthetase in Indian pulses

Experientia

416-417

1977

Lathyrus sativus, Lathyrus oleraceus, Lens sp., Cicer arietinum, Cajanus indicus, Glycine max, Vigna radiata var. radiata, Vigna unguiculata subsp. unguiculata

862718

brenda

Sehgal, D.; Santha, I.M.; Mehta, S.L.

Purification of oxalyl CoA synthetase enzyme from Lathyrus sativus and raising of antibodies

J. Plant Biochem. Biotechnol.

97-100

1992

Lathyrus sativus, Lathyrus sativus P-24

brenda

Giovanelli, J.

Oxalyl-coenzyme A synthetase from pea seed

Biochim. Biophys. Acta

118

124-143

1966

Lathyrus oleraceus, Lupinus sp., Pumpkin, Triticum aestivum

4288975

brenda

Foster, J.; Nakata, P.A.

An oxalyl-CoA synthetase is important for oxalate metabolism in Saccharomyces cerevisiae

FEBS Lett.

588

160-166

2014

Saccharomyces cerevisiae (P38137), Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741 (P38137)

24291261

brenda

Foster, J.; Kim, H.U.; Nakata, P.A.; Browse, J.

A previously unknown oxalyl-CoA synthetase is important for oxalate catabolism in Arabidopsis

Plant Cell

1217-1229

2012

Arabidopsis thaliana (Q9SMT7), Arabidopsis thaliana

22447686

brenda

Fan, M.; Xiao, Y.; Li, M.; Chang, W.

Crystal structures of Arabidopsis thaliana oxalyl-CoA synthetase essential for oxalate degradation

Mol. Plant

1349-1352

2016

Arabidopsis thaliana (Q9SMT7), Arabidopsis thaliana

27326693

brenda

Peng, C.; Liang, X.; Liu, E.E.; Zhang, J.J.; Peng, X.X.

The oxalyl-CoA synthetase-regulated oxalate and its distinct effects on resistance to bacterial blight and aluminium toxicity in rice

Plant Biol.

345-353

2017

Oryza sativa

28039904

brenda

Lou, H.Q.; Fan, W.; Xu, J.M.; Gong, Y.L.; Jin, J.F.; Chen, W.W.; Liu, L.Y.; Hai, M.R.; Yang, J.L.; Zheng, S.J.

An oxalyl-CoA synthetase is involved in oxalate degradation and aluminum tolerance

Plant Physiol.

172

1679-1690

2016

Vigna umbellata (A0A1C9HM85), Vigna umbellata

27650448

brenda

Foster, J.; Luo, B.; Nakata, P.A.

An oxalyl-CoA dependent pathway of oxalate catabolism plays a role in regulating calcium oxalate crystal accumulation and defending against oxalate-secreting phytopathogens in Medicago truncatula

PLoS ONE

e0149850

2016

Medicago truncatula

26900946

brenda

Li, P.; He, Q.; Jin, J.; Liu, Y.; Wen, Y.; Zhao, K.; Mao, G.; Fan, W.; Yang, J.

Tomato oxalyl-CoA synthetase degrades oxalate and affects fruit quality

Front. Plant Sci.

951386

2022

Solanum lycopersicum

35874016

brenda

Xian, P.; Cai, Z.; Cheng, Y.; Lin, R.; Lian, T.; Ma, Q.; Nian, H.

Wild soybean oxalyl-CoA synthetase degrades oxalate and affects the tolerance to cadmium and aluminum stresses

Int. J. Mol. Sci.

8869

2020

Glycine soja

33238600

brenda