Crystallization (Comment) | Organism |
---|---|
apo, ATP-bound, as well as oxalate- and AMP-bound enzyme states, hanging drop vapor diffusion method, using | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
H214A | the mutant shows strongly reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
H319A | the mutant shows strongly reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
K500A | the mutant shows strongly reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
R409A | the mutant shows strongly reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
S289A | the mutant shows strongly reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04215 | - |
oxalate | pH and temperature not specified in the publication | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required in form of Mg-ATP | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxalate + CoA | Arabidopsis thaliana | oxalate is the specific substrate | AMP + diphosphate + oxalyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SMT7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxalate + CoA | oxalate is the specific substrate | Arabidopsis thaliana | AMP + diphosphate + oxalyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAE3 | acyl-activating enzyme3, gene name | Arabidopsis thaliana |
Oxalyl-CoA synthetase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme-dependent degradation of oxalate is important for normal seed development and for defense against oxalate-producing fungal pathogens | Arabidopsis thaliana |