Cloned (Comment) | Organism |
---|---|
individual expression of His-tagged alpha- and beta-subunits in Escherichia coli strain BL21(DE3) | Aquifex aeolicus |
Protein Variants | Comment | Organism |
---|---|---|
K599A | site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity | Aquifex aeolicus |
K599A/K600A | site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity | Aquifex aeolicus |
K599A/K600A/K605A/K606A | site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity | Aquifex aeolicus |
K600A | site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity | Aquifex aeolicus |
K605A | site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity | Aquifex aeolicus |
K605A/K606A | site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity | Aquifex aeolicus |
K606A | site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity | Aquifex aeolicus |
additional information | construction of diverse truncation mutants, ATP-diphosphate-exchange and aminoacylation activities, and ability to charge minihelixLeu of alpha and beta subunit mutants, overview | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant trunacted mutants, overview | Aquifex aeolicus | |
0.0003 | - |
tRNALeuGAG | pH 6.8, 65°C, recombinant wild-type enzyme | Aquifex aeolicus | |
0.006 | - |
L-leucine | pH 6.8, 65°C, recombinant wild-type enzyme | Aquifex aeolicus | |
0.1123 | - |
ATP | pH 6.8, 65°C, recombinant wild-type enzyme | Aquifex aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | Aquifex aeolicus | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged alpha- and beta-subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | recombinant tRNALeu substrate, two peptides of eight and nine amino acid residues in the domain located in the alpha subunit are essential for the enzymes activity | Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeuGAG | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeuGAG | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | (alphabeta) heterodimer with a large extra domain, the leucine-specific domain, inserted into the catalytic domain, the subunit split site is exactly in the middle of the leucine-specific domain and may have a unique function, two peptides of eight and nine amino acid residues in the domain located in the alpha subunit are essential for the enzymes activity | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
Leucyl-tRNA synthetase | - |
Aquifex aeolicus |
LeuRS | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 65 | broad temperature optimum | Aquifex aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
L-leucine | pH 6.8, 65°C, recombinant wild-type enzyme | Aquifex aeolicus | |
1.5 | - |
tRNALeuGAG | pH 6.8, 65°C, recombinant wild-type enzyme | Aquifex aeolicus | |
1.81 | - |
ATP | pH 6.8, 65°C, recombinant wild-type enzyme | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Aquifex aeolicus |