Cloned (Comment) | Organism |
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gene LAS, map based cloning of LAS allele, a mapping population is derived from heterozygous plants (LAS/las) crossed with japonica cv. Nipponbare, quantitative real-time RT-PCR expression analysis. The LAS locus is mapped to the short arm of chromosome 2 to a 134 kb genomic DNA region | Oryza sativa Indica Group |
Protein Variants | Comment | Organism |
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additional information | the albinic las mutant is selected from an MNU-mutagenized population of indica cultivar N22, transgenic rice lines are produced by Agrobacterium tumefaciens-mediated co-cultivation. Albinic plants die at the fourth leaf stage presumably as seed nutrient reserves becomes exhausted, phenotype, overview. Expression levels of class I genes (PsbD1, PsaA1, PsaA2 and RBCL) in the las mutant are sharply reduced compared to wild type, class II genes (NADH2, NADH4, ATPCFalpha) are expressed normally, and expressions of class III genes (RpoA, RpoB, RpoC1 and RpoC2) are increased compared to wild-type. NEP accumulates in the las mutant, whereas those of genes transcribed by PEP are impaired. Phenotype, overview | Oryza sativa Indica Group |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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chloroplast | - |
Oryza sativa Indica Group | 9507 | - |
additional information | subcellular localization and expression analysis of LAS | Oryza sativa Indica Group | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Indica Group | B8BNI5 | cv. N22 | - |
Source Tissue | Comment | Organism | Textmining |
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leaf | gene LAS is constitutively expressed with relatively high level in leaves | Oryza sativa Indica Group | - |
seedling | - |
Oryza sativa Indica Group | - |
Synonyms | Comment | Organism |
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Threonyl-tRNA synthetase | - |
Oryza sativa Indica Group |
ThrRS | - |
Oryza sativa Indica Group |
General Information | Comment | Organism |
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malfunction | mutation of threonyl-tRNA synthetase results in a lethal albinic rice mutant las. las mutant plants show weak chlorophyll fluorescence, negligible chlorophyll accumulation, and defective thylakoid membrane development. Chloroplast-encoded protein levels are sharply reduced in the las mutant. Biogenesis of chloroplast rRNAs (16S and 23S rRNA) is arrested, leading to impaired translation and protein synthesis. Expression levels of class I genes (PsbD1, PsaA1, PsaA2 and RBCL) in the las mutant are sharply reduced compared to wild type, class II genes (NADH2, NADH4, ATPCFalpha) are expressed normally, and expressions of class III genes (RpoA, RpoB, RpoC1 and RpoC2) are increased compared to wild-type. NEP accumulates in the las mutant, whereas those of genes transcribed by PEP are impaired. The plastid ribosomal RNA biogenesis system is abnormal in las plants. Phenotype, overview | Oryza sativa Indica Group |
physiological function | threonyl-tRNA synthetase is essential for plant development by stabilizing of nuclear-encoded RNA polymerase (NEP) and plastid-encoded RNA polymerase (PEP) gene expressions and chloroplast protein synthesis. LAS is also essential for protein synthesis and construction of the ribosome system in rice chloroplasts | Oryza sativa Indica Group |