Information on EC 6.1.1.3 - threonine-tRNA ligase

Reaction Schemes

hideshow

ATP

+

L-threonine

+

tRNAThr

=

AMP

+

diphosphate

+

L-threonyl-tRNAThr

+

+

=

+

+

Synonyms
threonyl-trna synthetase, thrrs, thr-trna synthetase, mitochondrial threonyl-trna synthetase, threonyl trna synthetase, threonine-trna ligase, threonyl-transfer ribonucleic acid synthetase, bathrrs, apthrrs-1, apthrrs-2, more

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

ApThrRS-1

2 entries

ApThrRS-2

2 entries

BaThrRS

Brucella abortus

-

-

745113

EcThrRS

Escherichia coli

P0A8M3

-

745349

ectRNAThr

Escherichia coli

P0A8M3

-

745397

McThrRS

Mycoplasma capricolum

-

-

745349

mitochondrial threonyl-tRNA synthetase

2 entries

MJ1197

2 entries

MmThrRS

2 entries

Mst1

4 entries

Os02g33500

Oryza sativa Japonica Group

-

locus name

766847

ScmtThrRS

2 entries

SfThrRS-1

Sulfurisphaera tokodaii

-

protein contains the catalytic domain of ThrRS

690277

SfThrRS-2

Sulfurisphaera tokodaii

-

protein contains the editing domain of ThrRS

690277

Synthetase, threonyl-transfer ribonucleate

8 entries

TarS

3 entries

TARS1

2 entries

TARS3

Mus musculus

Q8BLY2

-

767637

TARSL2

Mus musculus

Q8BLY2

-

767637

Thr-tRNA synthetase

3 entries

Threonine translase

8 entries

Threonine--tRNA ligase

8 entries

Threonine-transfer ribonucleate synthetase

8 entries

threonyl tRNA synthetase

Escherichia coli

P0A8M3

-

745397

Threonyl-ribonucleic synthetase

8 entries

Threonyl-transfer ribonucleate synthetase

8 entries

Threonyl-transfer ribonucleic acid synthetase

8 entries

Threonyl-transfer RNA synthetase

8 entries

Threonyl-tRNA synthetase

33 entries

ThrRS

34 entries

ThrRS1

Aeropyrum pernix

-

-

726613

ThrS

12 entries

TRS

8 entries

additional information

2 entries

ApThrRS-1

Aeropyrum pernix

-

protein contains the catalytic domain of ThrRS

690277

ApThrRS-1

Aeropyrum pernix

Q9YDW0

-

705167

ApThrRS-2

Aeropyrum pernix

-

protein contains the editing domain of ThrRS

690277

ApThrRS-2

Aeropyrum pernix

Q9YFY3

-

705167

mitochondrial threonyl-tRNA synthetase

Candida albicans

-

-

716387

mitochondrial threonyl-tRNA synthetase

Saccharomyces cerevisiae

-

-

716387

MJ1197

Methanocaldococcus jannaschii

Q58597

-

751650

MJ1197

Methanocaldococcus jannaschii ATCC 43067

Q58597

-

-

MmThrRS

[Mycoplasma] mobile

Q6KH76

-

745349

MmThrRS

[Mycoplasma] mobile ATCC 43663

Q6KH76

-

-

Mst1

Candida albicans

-

-

716387

Mst1

Saccharomyces cerevisiae

-

-

716387, 727916, 728681, 743906

Mst1

Saccharomyces cerevisiae

P07236

-

745918

Mst1

Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

-

-

ScmtThrRS

Saccharomyces cerevisiae

P07236

-

745918

ScmtThrRS

Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

-

-

Synthetase, threonyl-transfer ribonucleate

-

-

-

-

Synthetase, threonyl-transfer ribonucleate

Aeropyrum pernix

Q9YFY3

-

654016

Synthetase, threonyl-transfer ribonucleate

Cricetulus griseus

-

-

652046

Synthetase, threonyl-transfer ribonucleate

Escherichia coli

-

-

650200, 650778, 650784, 653273

Synthetase, threonyl-transfer ribonucleate

Escherichia coli

P0A8M3

-

653312, 653324

Synthetase, threonyl-transfer ribonucleate

Haloferax volcanii

-

-

654016

Synthetase, threonyl-transfer ribonucleate

Oryctolagus cuniculus

-

-

652046

Synthetase, threonyl-transfer ribonucleate

Staphylococcus aureus

Q8NW68

-

652919

TarS

Danio rerio

A2BIM7

-

744233

TarS

Homo sapiens

P26639

-

744595

TarS

Mus musculus

Q9D0R2

-

767637

TARS1

Homo sapiens

P26639

-

767564

TARS1

Mus musculus

Q9D0R2

-

766659, 767637

Thr-tRNA synthetase

Arabidopsis thaliana

-

-

705818

Thr-tRNA synthetase

Arabidopsis thaliana

F4IFC5

-

744913

Thr-tRNA synthetase

Methanosarcina mazei

-

-

663379

Threonine translase

-

-

-

-

Threonine translase

Aeropyrum pernix

Q9YFY3

-

654016

Threonine translase

Cricetulus griseus

-

-

652046

Threonine translase

Escherichia coli

-

-

650200, 650778, 650784, 653273

Threonine translase

Escherichia coli

P0A8M3

-

653312, 653324

Threonine translase

Haloferax volcanii

-

-

654016

Threonine translase

Oryctolagus cuniculus

-

-

652046

Threonine translase

Staphylococcus aureus

Q8NW68

-

652919

Threonine--tRNA ligase

-

-

-

-

Threonine--tRNA ligase

Aeropyrum pernix

Q9YFY3

-

654016

Threonine--tRNA ligase

Cricetulus griseus

-

-

652046

Threonine--tRNA ligase

Escherichia coli

-

-

650200, 650778, 650784, 653273

Threonine--tRNA ligase

Escherichia coli

P0A8M3

-

653312, 653324

Threonine--tRNA ligase

Haloferax volcanii

-

-

654016

Threonine--tRNA ligase

Oryctolagus cuniculus

-

-

652046

Threonine--tRNA ligase

Staphylococcus aureus

Q8NW68

-

652919

Threonine-transfer ribonucleate synthetase

-

-

-

-

Threonine-transfer ribonucleate synthetase

Aeropyrum pernix

Q9YFY3

-

654016

Threonine-transfer ribonucleate synthetase

Cricetulus griseus

-

-

652046

Threonine-transfer ribonucleate synthetase

Escherichia coli

-

-

650200, 650778, 650784, 653273

Threonine-transfer ribonucleate synthetase

Escherichia coli

P0A8M3

-

653312, 653324

Threonine-transfer ribonucleate synthetase

Haloferax volcanii

-

-

654016

Threonine-transfer ribonucleate synthetase

Oryctolagus cuniculus

-

-

652046

Threonine-transfer ribonucleate synthetase

Staphylococcus aureus

Q8NW68

-

652919

Threonyl-ribonucleic synthetase

-

-

-

-

Threonyl-ribonucleic synthetase

Aeropyrum pernix

Q9YFY3

-

654016

Threonyl-ribonucleic synthetase

Cricetulus griseus

-

-

652046

Threonyl-ribonucleic synthetase

Escherichia coli

-

-

650200, 650778, 650784, 653273

Threonyl-ribonucleic synthetase

Escherichia coli

P0A8M3

-

653312, 653324

Threonyl-ribonucleic synthetase

Haloferax volcanii

-

-

654016

Threonyl-ribonucleic synthetase

Oryctolagus cuniculus

-

-

652046

Threonyl-ribonucleic synthetase

Staphylococcus aureus

Q8NW68

-

652919

Threonyl-transfer ribonucleate synthetase

-

-

-

-

Threonyl-transfer ribonucleate synthetase

Aeropyrum pernix

Q9YFY3

-

654016

Threonyl-transfer ribonucleate synthetase

Cricetulus griseus

-

-

652046

Threonyl-transfer ribonucleate synthetase

Escherichia coli

-

-

650200, 650778, 650784, 653273

Threonyl-transfer ribonucleate synthetase

Escherichia coli

P0A8M3

-

653312, 653324

Threonyl-transfer ribonucleate synthetase

Haloferax volcanii

-

-

654016

Threonyl-transfer ribonucleate synthetase

Oryctolagus cuniculus

-

-

652046

Threonyl-transfer ribonucleate synthetase

Staphylococcus aureus

Q8NW68

-

652919

Threonyl-transfer ribonucleic acid synthetase

-

-

-

-

Threonyl-transfer ribonucleic acid synthetase

Aeropyrum pernix

Q9YFY3

-

654016

Threonyl-transfer ribonucleic acid synthetase

Cricetulus griseus

-

-

652046

Threonyl-transfer ribonucleic acid synthetase

Escherichia coli

-

-

650200, 650778, 650784, 653273

Threonyl-transfer ribonucleic acid synthetase

Escherichia coli

P0A8M3

-

653312, 653324

Threonyl-transfer ribonucleic acid synthetase

Haloferax volcanii

-

-

654016

Threonyl-transfer ribonucleic acid synthetase

Oryctolagus cuniculus

-

-

652046

Threonyl-transfer ribonucleic acid synthetase

Staphylococcus aureus

Q8NW68

-

652919

Threonyl-transfer RNA synthetase

-

-

-

-

Threonyl-transfer RNA synthetase

Aeropyrum pernix

Q9YFY3

-

654016

Threonyl-transfer RNA synthetase

Cricetulus griseus

-

-

652046

Threonyl-transfer RNA synthetase

Escherichia coli

-

-

650200, 650778, 650784, 653273

Threonyl-transfer RNA synthetase

Escherichia coli

P0A8M3

-

653312, 653324

Threonyl-transfer RNA synthetase

Haloferax volcanii

-

-

654016

Threonyl-transfer RNA synthetase

Oryctolagus cuniculus

-

-

652046

Threonyl-transfer RNA synthetase

Staphylococcus aureus

Q8NW68

-

652919

Threonyl-tRNA synthetase

-

-

-

-

Threonyl-tRNA synthetase

Aeropyrum pernix

-

-

690277, 726613, 728382

Threonyl-tRNA synthetase

Aeropyrum pernix

Q9YDW0

-

705167

Threonyl-tRNA synthetase

Aeropyrum pernix

Q9YFY3

-

654016, 705167

Threonyl-tRNA synthetase

Aeropyrum pernix DSM 11879

-

-

-

Threonyl-tRNA synthetase

Archaeoglobus fulgidus

-

-

662443

Threonyl-tRNA synthetase

Brucella abortus

-

-

745113

Threonyl-tRNA synthetase

Burkholderia thailandensis

-

-

728097

Threonyl-tRNA synthetase

Cricetulus griseus

-

-

652046

Threonyl-tRNA synthetase

Danio rerio

A2BIM7

-

744233

Threonyl-tRNA synthetase

Escherichia coli

-

-

650200, 650778, 650784, 653273, 714230, 715520, 716771, 728097

Threonyl-tRNA synthetase

Escherichia coli

P0A8M3

-

653312, 653324, 662443, 662829, 663398, 728193, 744225, 745349, 745920

Threonyl-tRNA synthetase

Escherichia coli K-12

-

-

674339

Threonyl-tRNA synthetase

Escherichia coli MG1655

-

-

-

Threonyl-tRNA synthetase

Halobacterium sp.

-

-

662443

Threonyl-tRNA synthetase

Haloferax volcanii

-

-

654016

Threonyl-tRNA synthetase

Helicobacter pylori

-

-

662443

Threonyl-tRNA synthetase

Homo sapiens

P26639

-

728097, 744595

Threonyl-tRNA synthetase

Methanocaldococcus jannaschii

-

-

662443

Threonyl-tRNA synthetase

Mycoplasma capricolum

-

-

745349

Threonyl-tRNA synthetase

Oryctolagus cuniculus

-

-

652046

Threonyl-tRNA synthetase

Oryza sativa Indica Group

B8BNI5

-

746059

Threonyl-tRNA synthetase

Pyrococcus abyssi

-

-

660587

Threonyl-tRNA synthetase

Pyrococcus abyssi

Q9UZ14

-

673326, 704188

Threonyl-tRNA synthetase

Saccharolobus solfataricus

-

-

662443

Threonyl-tRNA synthetase

Saccharomyces cerevisiae

-

-

727916, 728681, 743906

Threonyl-tRNA synthetase

Saccharomyces cerevisiae

P07236

-

745918

Threonyl-tRNA synthetase

Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

-

-

Threonyl-tRNA synthetase

Staphylococcus aureus

Q8NW68

-

652919

Threonyl-tRNA synthetase

Sulfurisphaera tokodaii

-

-

690277

Threonyl-tRNA synthetase

Yersinia pestis

-

-

728097

Threonyl-tRNA synthetase

[Mycoplasma] mobile

Q6KH76

-

745349

Threonyl-tRNA synthetase

[Mycoplasma] mobile ATCC 43663

Q6KH76

-

-

ThrRS

-

-

-

-

ThrRS

Aeropyrum pernix

-

-

728383

ThrRS

Aeropyrum pernix

Q9YDW0

-

705167

ThrRS

Aeropyrum pernix

Q9YFY3

-

654016, 705167

ThrRS

Aeropyrum pernix DSM 11879

-

-

-

ThrRS

Arabidopsis thaliana

-

-

705818

ThrRS

Arabidopsis thaliana

F4IFC5

-

744913

ThrRS

Archaeoglobus fulgidus

-

-

662443

ThrRS

Brucella abortus

-

-

745113

ThrRS

Burkholderia thailandensis

-

-

728097

ThrRS

Cricetulus griseus

-

-

652046

ThrRS

Escherichia coli

-

-

650200, 650778, 650784, 653273, 714230, 715520, 716771, 728097

ThrRS

Escherichia coli

P0A8M3

-

653312, 653324, 662443, 662829, 728193, 744225, 745349, 745920

ThrRS

Escherichia coli MG1655

-

-

-

ThrRS

Halobacterium sp.

-

-

662443

ThrRS

Haloferax volcanii

-

-

654016

ThrRS

Helicobacter pylori

-

-

662443

ThrRS

Homo sapiens

P26639

-

728097

ThrRS

Methanocaldococcus jannaschii

-

-

662443

ThrRS

Methanocaldococcus jannaschii

Q58597

-

663210

ThrRS

Methanosarcina mazei

-

-

663379

ThrRS

Mycoplasma capricolum

-

-

745349

ThrRS

Oryctolagus cuniculus

-

-

652046

ThrRS

Oryza sativa Indica Group

B8BNI5

-

746059

ThrRS

Pyrococcus abyssi

-

-

660587

ThrRS

Pyrococcus abyssi

Q9UZ14

-

673326, 704188

ThrRS

Saccharolobus solfataricus

-

-

662443

ThrRS

Saccharolobus solfataricus

Q980D1

-

663210

ThrRS

Saccharomyces cerevisiae

P07236

-

745918

ThrRS

Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

-

-

ThrRS

Staphylococcus aureus

Q8NW68

-

652919

ThrRS

Yersinia pestis

-

-

728097

ThrRS

[Mycoplasma] mobile

Q6KH76

-

745349

ThrRS

[Mycoplasma] mobile ATCC 43663

Q6KH76

-

-

ThrS

Brucella abortus

-

-

745113

ThrS

Escherichia coli

P0A8M3

-

744225, 745349, 745920

ThrS

Methanocaldococcus jannaschii

Q58597

-

751650

ThrS

Methanocaldococcus jannaschii ATCC 43067

Q58597

-

-

ThrS

Mycoplasma capricolum

-

-

745349

ThrS

Pyrococcus abyssi

Q9UZ14

-

751650, 767331

ThrS

Pyrococcus furiosus

Q8U176

-

751650

ThrS

Pyrococcus furiosus ATCC 43587

Q8U176

-

-

ThrS

Staphylococcus aureus

Q2FXP7

-

765920

ThrS

Staphylococcus aureus NCTC 8325

Q2FXP7

-

-

ThrS

[Mycoplasma] mobile

Q6KH76

-

745349

ThrS

[Mycoplasma] mobile ATCC 43663

Q6KH76

-

-

TRS

-

-

-

-

TRS

Aeropyrum pernix

Q9YFY3

-

654016

TRS

Cricetulus griseus

-

-

652046

TRS

Escherichia coli

-

-

650200, 650778, 650784, 653273

TRS

Escherichia coli

P0A8M3

-

653312, 653324

TRS

Haloferax volcanii

-

-

654016

TRS

Oryctolagus cuniculus

-

-

652046

TRS

Staphylococcus aureus

Q8NW68

-

652919

additional information

Escherichia coli

-

the enzyme belongs to the class II aminoacyl-tRNA synthetases

714230

additional information

Escherichia coli

-

the enzyme belongs to the class II aminoacyl-tRNA synthetases

715520

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

12 entries

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

bi uni uni bi ping-pong mechanism

-

-

-

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

mechanism, active site structure, and substrate recognition method

Escherichia coli

-

650784

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

-

-

-

-

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

functional mechanism and substrate recognition, editing model, 2 separate active sites for substrate binding, binding of tRNA is more specific than the binding of the amino acid

Escherichia coli

-

650778

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

active site structure

Escherichia coli

P0A8M3

653312

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

substrate binding and mechanism

Staphylococcus aureus

Q8NW68

652919

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

induced fit, Trp434 is involved in conformational changes during substrate binding

Escherichia coli

-

650200

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

the catalytic domain is located at the C-terminus of the enzyme

Escherichia coli

-

653273

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

reaction mechanism analysis by QM/MM and MM modeling of full length and truncated enzyme, molecular mechanism simulation

Escherichia coli

P0A8M3

663398

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

determination of substrate binding sites, catalytic sites, and catalytic mechanism from structure modeling

Methanosarcina mazei

-

663379

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

reaction mechanism and structure-function relationship, modeling, overview

Saccharomyces cerevisiae

-

743906

ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr

reaction mechanism and structure-function relationship, overview. Molecular dynamics simulation study of the dynamics of the active site organization during charging step of dimeric ThrRS from Escherichia coli (ecThrRS) bound with ectRNAThr

Escherichia coli

P0A8M3

745397

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Aminoacylation

14 entries

esterification

11 entries

Aminoacylation

-

-

-

-

Aminoacylation

Escherichia coli

-

-

650784

Aminoacylation

Aeropyrum pernix

Q9YDW0, Q9YFY3

-

654016

Aminoacylation

Cricetulus griseus

-

-

652046

Aminoacylation

Oryctolagus cuniculus

-

-

652046

Aminoacylation

Escherichia coli

-

-

650778

Aminoacylation

Escherichia coli

P0A8M3

-

653312, 653324

Aminoacylation

Escherichia coli

-

-

653324

Aminoacylation

Staphylococcus aureus

Q8NW68

-

652919

Aminoacylation

Escherichia coli

-

-

650200, 653273

Aminoacylation

Haloferax volcanii

-

-

654016

Aminoacylation

Pyrococcus abyssi

Q9UZ14

-

704188

Aminoacylation

Aeropyrum pernix

Q9YDW0, Q9YFY3

-

705167

Aminoacylation

Arabidopsis thaliana

-

-

705818

esterification

-

-

-

-

esterification

Escherichia coli

-

-

650784

esterification

Aeropyrum pernix

Q9YDW0, Q9YFY3

-

654016

esterification

Cricetulus griseus

-

-

652046

esterification

Oryctolagus cuniculus

-

-

652046

esterification

Escherichia coli

-

-

650778

esterification

Escherichia coli

P0A8M3

-

653312, 653324

esterification

Escherichia coli

-

-

653324

esterification

Staphylococcus aureus

Q8NW68

-

652919

esterification

Escherichia coli

-

-

650200, 653273

esterification

Haloferax volcanii

-

-

654016

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PATHWAY SOURCE

PATHWAYS

BRENDA

threonine metabolism

KEGG

Aminoacyl-tRNA biosynthesis

MetaCyc

tRNA charging

top print hide Go to Systematic Name Search

SYSTEMATIC NAME

IUBMB Comments

L-threonine:tRNAThr ligase (AMP-forming)

-

top print hide show all columns Go to CAS Registry Number Search

CAS REGISTRY NUMBER

COMMENTARY

9023-46-5

-

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

Reversibility
r=reversible
ir=irreversible
?=not specified

2-Chloroadenosine 5'-triphosphate + L-threonine + tRNAThr

?

2 entries

AMP + diphosphate + L-threonyl-tRNAThr

ATP + L-threonine + tRNAThr

Saccharomyces pastorianus

-

409

Substrates: -
Products: -

r

ATP + 3-hydroxynorvaline + tRNAThr

AMP + diphosphate + 3-hydroxynorvalyl-tRNAThr

Escherichia coli

-

714230

Substrates: the specificity constant kcat/KM for beta-hydroxynorvaline is only 20-30fold less than that of cognate threonine, amino acid activation is the potential rate-limiting step of b3-hydroxynorvaline aminoacylation
Products: -

?

ATP + hydroxynorvaline + tRNAThr

AMP + diphosphate + hydroxynorvalyl-tRNAThr

Escherichia coli

P0A8M3

653312

Substrates: 10-70% less active than with L-threonine
Products: -

?

ATP + L-isoleucine + tRNAIle

AMP + diphosphate + L-isoleucyl-tRNAIle

2 entries

ATP + L-serine + tRNASer

?

Saccharomyces cerevisiae

-

727916

Substrates: yeast mitochondrial threonyl-tRNA synthetase MST1 lacks an editing domain and utilizes pre-transfer editing to discriminate against serine. MST1 misactivates serine and edits seryl adenylate (Ser-AMP) in the absence of the cognate tRNA. MST1 hydrolyzes 80% of misactivated Ser-AMP at a rate 4fold higher than that for the cognate threonyl adenylate (Thr-AMP) while releasing 20% of Ser-AMP into the solution.
Products: -

?

ATP + L-serine + tRNASer

AMP + diphosphate + L-seryl-tRNASer

4 entries

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

10 entries

ATP + L-threonine + tRNA1Thr

AMP + diphosphate + L-threonyl-tRNA1Thr

Saccharomyces cerevisiae

-

728681

Substrates: -
Products: -

?

ATP + L-threonine + tRNA2Thr

AMP + diphosphate + L-threonyl-tRNA2Thr

Saccharomyces cerevisiae

-

728681

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

?

Thermus thermophilus

-

398

Substrates: catalyzes the attachment of threonine onto its cognate tRNA molecule, prior to participation of the aminoacylated tRNA in the protein synthesis
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

83 entries

ATP + L-threonine + tRNAThr1

AMP + diphosphate + L-threonyl-tRNAThr1

4 entries

ATP + L-threonine + tRNAThr2

AMP + diphosphate + L-threonyl-tRNAThr2

2 entries

Formycin 5'-triphosphate + L-threonine + tRNAThr

?

2 entries

additional information

?

-

38 entries

2-Chloroadenosine 5'-triphosphate + L-threonine + tRNAThr

?

Rattus norvegicus

-

411

Substrates: -
Products: -

?

2-Chloroadenosine 5'-triphosphate + L-threonine + tRNAThr

?

Saccharomyces cerevisiae

-

273

Substrates: -
Products: -

?

ATP + L-isoleucine + tRNAIle

AMP + diphosphate + L-isoleucyl-tRNAIle

Oryctolagus cuniculus

-

652046

Substrates: -
Products: -

?

ATP + L-isoleucine + tRNAIle

AMP + diphosphate + L-isoleucyl-tRNAIle

Oryctolagus cuniculus

-

652046

Substrates: the reaction catalyzed by the enzyme plays an important role in the transport of aminoacylated tRNAs from the nucleus to the cytoplasm
Products: -

?

ATP + L-serine + tRNASer

AMP + diphosphate + L-seryl-tRNASer

Burkholderia thailandensis

-

728097

Substrates: -
Products: -

?

ATP + L-serine + tRNASer

AMP + diphosphate + L-seryl-tRNASer

Escherichia coli

-

728097

Substrates: -
Products: -

?

ATP + L-serine + tRNASer

AMP + diphosphate + L-seryl-tRNASer

Homo sapiens

P26639

728097

Substrates: -
Products: -

?

ATP + L-serine + tRNASer

AMP + diphosphate + L-seryl-tRNASer

Yersinia pestis

-

728097

Substrates: -
Products: -

?

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

Escherichia coli

P0A8M3

653312

Substrates: 1000fold less active than with L-threonine
Products: -

?

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

Escherichia coli

-

650778

Substrates: very low activity with the wild-type enzyme
Products: -

?

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

Escherichia coli

P0A8M3

662829

Substrates: low activity
Products: -

r

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

Methanocaldococcus jannaschii

Q58597

663210

Substrates: L-serine is a poor substrate for the wild-type enzyme
Products: -

r

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

Mus musculus

Q8BLY2, Q9D0R2

767637

Substrates: -
Products: -

?

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

Mycoplasma capricolum

-

745349

Substrates: reaction of EC 6.1.1.11, mischarging of tRNAThr
Products: -

?

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

Pyrococcus abyssi

Q9UZ14

767331

Substrates: -
Products: -

?

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

Saccharolobus solfataricus

Q980D1

663210

Substrates: -
Products: -

?

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

[Mycoplasma] mobile

Q6KH76

745349

Substrates: reaction of EC 6.1.1.11, mischarging of tRNAThr, low activity
Products: -

?

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

[Mycoplasma] mobile ATCC 43663

Q6KH76

745349

Substrates: reaction of EC 6.1.1.11, mischarging of tRNAThr, low activity
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix

Q9YDW0, Q9YFY3

654016

Substrates: secondary structure of the tRNAThr, enzyme utilizes substrates from archaeon with discriminator base U73 and Escherichia coli with discriminator base A73
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix

Q9YDW0, Q9YFY3

654016

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix

Q9YDW0, Q9YFY3

705167

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix

-

728382, 726613

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix

-

728383

Substrates: the enzyme recognizes the first three base pairs of acceptor stem in addition to the second and the third letters of anticodon of tRNA(Thr). The discriminator base is not involved in recognition by the enzyme
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix

-

728382

Substrates: the enzyme threonylates not only archaeal (Aeropyrum pernix and Haloferax volcanii) threonine tRNAs but also Escherichia coli threonine tRNA
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix

-

728382

Substrates: the enzyme from Aeropyrum pernix threonylated threonine tRNAs from Aeropyrum pernix, Haloferax volcanii and Escherichia coli
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix DSM 11879

-

728383

Substrates: the enzyme recognizes the first three base pairs of acceptor stem in addition to the second and the third letters of anticodon of tRNA(Thr). The discriminator base is not involved in recognition by the enzyme
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix DSM 11879

-

728382

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix DSM 11879

-

728382

Substrates: the enzyme threonylates not only archaeal (Aeropyrum pernix and Haloferax volcanii) threonine tRNAs but also Escherichia coli threonine tRNA
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix DSM 11879

-

728382

Substrates: the enzyme from Aeropyrum pernix threonylated threonine tRNAs from Aeropyrum pernix, Haloferax volcanii and Escherichia coli
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aesculus hippocastanum

-

395

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Arabidopsis thaliana

-

705818

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Arabidopsis thaliana

F4IFC5

744913

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Archaeoglobus fulgidus

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Bos taurus

-

92, 395

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Brucella abortus

-

745113

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Burkholderia thailandensis

-

728097

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Candida albicans

-

716387

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Cricetulus griseus

-

413, 652046

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Cricetulus griseus

-

652046

Substrates: the reaction catalyzed by the enzyme plays an important role in the transport of aminoacylated tRNAs from the nucleus to the cytoplasm
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

395, 397, 406, 407, 650200, 650778, 650784

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

653312, 653324

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

653324

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

653273

Substrates: tRNA aminoacylation
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

653273

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

653324

Substrates: regulatory mechanism
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

663398, 662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

662829

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

663398

Substrates: via AMP-L-threonine-enzyme intermediate in a two-step process
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

714230, 715520, 716771

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

714230

Substrates: for cognate threonine, amino acid activation is likely to be the rate-limiting step. The inability of wild-type ThrRS to prevent utilization of beta-hydroxynorvaline as a substrate illustrates that the naturally occurring enzyme lacks the capability to effectively discriminate against nonproteogenic amino acids that are not encountered under normal physiological conditions
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

728193

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

728097

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

745920, 744225

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

744225

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

745349, 745397

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

745397

Substrates: Escherichia coli ectRNAThr
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

397

Substrates: tRNAThr3 of Escherichia coli
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli K-12

-

674339

Substrates: the enzyme acts as both an enzyme and a regulator of gene expression, it aminoacylates tRNAThr isoacceptors and binds to its own mRNA, inhibiting its translation, overview
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli K-12

-

674339

Substrates: the zinc atom in the active site is essential for the recognition of threonine
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli MG1655

-

716771

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli overproducing

-

407

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Geobacillus stearothermophilus

-

395

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Halobacterium sp.

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Haloferax volcanii

-

654016

Substrates: secondary structure of the tRNAThr, specific for archaeal tRNAThr with discriminator base U73, no activity with one of Escherichia coli with discriminator base A73
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Haloferax volcanii

-

654016, 728410

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Haloferax volcanii

-

728410

Substrates: Escherichia coli threonine tRNA is not aminoacylated by the Haloferax volcanii enzyme. The Escherichia coli mutant tRNAThr having U73 is threonylated by the Haloferax volcanii enzyme. The discriminator base U73 of Haloferax volcanii tRNAThr is a strong determinant for the recognition by threonyl-tRNA synthetase
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Helicobacter pylori

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Homo sapiens

-

395, 400

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Homo sapiens

P26639

728097, 744595

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Homo sapiens

-

744595

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Methanocaldococcus jannaschii

Q58597

663210

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Methanocaldococcus jannaschii

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Methanosarcina mazei

-

663379

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Methanosarcina mazei

-

663379

Substrates: determination of substrate binding sites, catalytic sites, and catalytic mechanism
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Mus musculus

-

395

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Mus musculus

Q8BLY2, Q9D0R2

767637

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Mycoplasma capricolum

-

745349

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Oryctolagus cuniculus

-

395, 415

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Pyrococcus abyssi

-

660587

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Pyrococcus abyssi

-

660587

Substrates: editing mechanism
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Pyrococcus abyssi

Q9UZ14

673326

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Pyrococcus abyssi

Q9UZ14

673326

Substrates: the main chains atoms of Tyr119 and Tyr120 are sufficient to prevent the deacylation of Thr-tRNAThr
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Pyrococcus abyssi

Q9UZ14

704188

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Rattus norvegicus

-

169, 395, 396, 403, 404, 405, 411

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Saccharolobus solfataricus

Q980D1

663210

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Saccharolobus solfataricus

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Saccharomyces cerevisiae

-

273, 395, 399, 402, 414, 716387

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Saccharomyces cerevisiae

-

716387

Substrates: MST1 can attach threonine to both tRNAThr1 and the regular tRNAThr2, but not to the wild-type tRNAHis. But a loss of the first nucleotide G-1 in tRNAHis converts it to a substrate for MST1
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Saccharomyces cerevisiae

-

727916, 728681, 743906

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Saccharomyces cerevisiae D273-10B

-

414

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Saccharomyces pastorianus

-

395, 409

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Staphylococcus aureus

Q8NW68

652919

Substrates: substrate binding induces conformational changes
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Staphylococcus aureus

Q8NW68

652919

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Thermus thermophilus

-

395, 397, 398, 401

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Thermus thermophilus

-

397

Substrates: tRNAThr of Thermus thermophilus
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Thermus thermophilus HB8 / ATCC 27634 / DSM 579

-

397

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Thermus thermophilus HB8 / ATCC 27634 / DSM 579

-

397

Substrates: tRNAThr of Thermus thermophilus
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Yersinia pestis

-

728097

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

[Mycoplasma] mobile

Q6KH76

745349

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

[Mycoplasma] mobile ATCC 43663

Q6KH76

745349

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr1

AMP + diphosphate + L-threonyl-tRNAThr1

Saccharomyces cerevisiae

P07236

745918

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr1

AMP + diphosphate + L-threonyl-tRNAThr1

Saccharomyces cerevisiae

P07236

745918

Substrates: specific binding mode of mitochondrial tRNAThr1 to mitochondrial ScmtThrRS
Products: -

?

ATP + L-threonine + tRNAThr1

AMP + diphosphate + L-threonyl-tRNAThr1

Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

745918

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr1

AMP + diphosphate + L-threonyl-tRNAThr1

Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

745918

Substrates: specific binding mode of mitochondrial tRNAThr1 to mitochondrial ScmtThrRS
Products: -

?

ATP + L-threonine + tRNAThr2

AMP + diphosphate + L-threonyl-tRNAThr2

Saccharomyces cerevisiae

P07236

745918

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr2

AMP + diphosphate + L-threonyl-tRNAThr2

Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

745918

Substrates: -
Products: -

?

Formycin 5'-triphosphate + L-threonine + tRNAThr

?

Rattus norvegicus

-

411

Substrates: -
Products: -

?

Formycin 5'-triphosphate + L-threonine + tRNAThr

?

Saccharomyces cerevisiae

-

273

Substrates: -
Products: -

?

additional information

?

-

Arabidopsis thaliana

F4IFC5

744913

Substrates: interaction of the dual targeting peptide of Thr-tRNA synthetase with the chloroplastic receptor Toc34 in Arabidopsis thaliana as a function of AtToc34 concentration. Mapping the AtToc34 interaction sites in AtThrRS-dTP(2-60), overview
Products: -

?

additional information

?

-

Arabidopsis thaliana

-

744913

Substrates: interaction of the dual targeting peptide of Thr-tRNA synthetase with the chloroplastic receptor Toc34 in Arabidopsis thaliana as a function of AtToc34 concentration. Mapping the AtToc34 interaction sites in AtThrRS-dTP(2-60), overview
Products: -

?

additional information

?

-

Cricetulus griseus

-

652046

Substrates: aminoacyl-tRNA is channeled in vivo by probably direct transfer to elongation factor I
Products: -

?

additional information

?

-

Escherichia coli

-

653273

Substrates: enzyme also has a regulatory function by binding the so-called operator site located in the leader of its own mRNA and thereby inhibits translational initiation by competing with ribosome binding
Products: -

?

additional information

?

-

Escherichia coli

P0A8M3

653312

Substrates: no activity with L-valine, determination of amino acid activation and discriminating editing mechanism
Products: -

?

additional information

?

-

Escherichia coli

P0A8M3

653324

Substrates: the enzyme represses the translation of its own mRNA by binding to an operator located upstream of the initiation codon thereby using the recognition mode of the tRNA anticodon loop to initiate binding
Products: -

?

additional information

?

-

Escherichia coli

-

653324

Substrates: the enzyme represses the translation of its own mRNA by binding to an operator located upstream of the initiation codon thereby using the recognition mode of the tRNA anticodon loop to initiate binding
Products: -

?

additional information

?

-

Escherichia coli

-

653273

Substrates: regulation mechanism, 2 essential steps of regulation are operator recognition and inhibition of ribosome binding performed by different domains of the enzyme
Products: -

?

additional information

?

-

Escherichia coli

P0A8M3

662829

Substrates: the enzyme needs to discriminate between threonine, serine, and valine in vivo, mechanism of proofreading of threonyl-tRNA synthetase at atomic resolution, overview
Products: -

?

additional information

?

-

Escherichia coli

-

715520

Substrates: in the pre-steady state, asymmetric activation of cognate threonine and noncognate serine is observed in the active sites of dimeric ThrRS, with similar rates of activation. In the absence of tRNA, seryl-adenylate is hydrolyzed 29old faster by the ThrRS catalytic domain than threonyl-adenylate. The rate of seryl transfer to cognate tRNA is only 2fold slower than threonine
Products: -

?

additional information

?

-

Escherichia coli

P0A8M3

745397

Substrates: the interaction of ecRNAThr with the enzyme, interactions between the catalytically important loops and tRNA contribute to the change in dynamics of tRNA in free and bound states, respectively. Presence of bound Mg2+ ions around tRNA and dynamically slow bound water are other common features of the enzyme
Products: -

?

additional information

?

-

Methanocaldococcus jannaschii

Q58597

663210

Substrates: a freestanding proofreading domain is required for protein synthesis quality control in archaea
Products: -

?

additional information

?

-

Methanocaldococcus jannaschii

Q58597

663210

Substrates: the N-terminal enzyme domain is responsible for editing
Products: -

?

additional information

?

-

Methanocaldococcus jannaschii

Q58597

751650

Substrates: proofreading modules of aminoacyl-tRNA synthetases are responsible for enforcing a high fidelity during translation of the genetic code. They use strategically positioned side chains for specifically targeting incorrect aminoacyl-tRNAs. A unique proofreading module possessing a D-aminoacyl-tRNA deacylase fold does not use side chains for imparting specificity or for catalysis, the two hallmark activities of enzymes
Products: -

?

additional information

?

-

Methanocaldococcus jannaschii ATCC 43067

Q58597

751650

Substrates: proofreading modules of aminoacyl-tRNA synthetases are responsible for enforcing a high fidelity during translation of the genetic code. They use strategically positioned side chains for specifically targeting incorrect aminoacyl-tRNAs. A unique proofreading module possessing a D-aminoacyl-tRNA deacylase fold does not use side chains for imparting specificity or for catalysis, the two hallmark activities of enzymes
Products: -

?

additional information

?

-

Methanosarcina mazei

-

663379

Substrates: structure-based evolutionary considerations
Products: -

?

additional information

?

-

Oryctolagus cuniculus

-

415

Substrates: enzyme can interact with high-MW RNAs
Products: -

?

additional information

?

-

Oryctolagus cuniculus

-

652046

Substrates: aminoacyl-tRNA is channeled in vivo by probably direct transfer to elongation factor I
Products: -

?

additional information

?

-

Pyrococcus abyssi

Q9UZ14

673326

Substrates: mischarging of the enzyme with noncognate amino acids, overview, post-transfer editing mechanism of the D-aminoacyl-tRNA deacylase-like domain in the archaeal threonyl-tRNA synthetase, mechanistic insights into the removal of noncognate L-serine from tRNAThr, M129 is responsible for enantiomeric selection in DTD, Glu134 is involved in fixing the seryl moiety in the active site, overview
Products: -

?

additional information

?

-

Pyrococcus abyssi

-

673326

Substrates: mischarging of the enzyme with noncognate amino acids, overview, post-transfer editing mechanism of the D-aminoacyl-tRNA deacylase-like domain in the archaeal threonyl-tRNA synthetase, mechanistic insights into the removal of noncognate L-serine from tRNAThr, M129 is responsible for enantiomeric selection in DTD, Glu134 is involved in fixing the seryl moiety in the active site, overview
Products: -

?

additional information

?

-

Pyrococcus abyssi

Q9UZ14

751650

Substrates: proofreading modules of aminoacyl-tRNA synthetases are responsible for enforcing a high fidelity during translation of the genetic code. They use strategically positioned side chains for specifically targeting incorrect aminoacyl-tRNAs. A unique proofreading module possessing a D-aminoacyl-tRNA deacylase fold does not use side chains for imparting specificity or for catalysis, the two hallmark activities of enzymes
Products: -

?

additional information

?

-

Pyrococcus furiosus

Q8U176

751650

Substrates: proofreading modules of aminoacyl-tRNA synthetases are responsible for enforcing a high fidelity during translation of the genetic code. They use strategically positioned side chains for specifically targeting incorrect aminoacyl-tRNAs. A unique proofreading module possessing a D-aminoacyl-tRNA deacylase fold does not use side chains for imparting specificity or for catalysis, the two hallmark activities of enzymes
Products: -

?

additional information

?

-

Pyrococcus furiosus ATCC 43587

Q8U176

751650

Substrates: proofreading modules of aminoacyl-tRNA synthetases are responsible for enforcing a high fidelity during translation of the genetic code. They use strategically positioned side chains for specifically targeting incorrect aminoacyl-tRNAs. A unique proofreading module possessing a D-aminoacyl-tRNA deacylase fold does not use side chains for imparting specificity or for catalysis, the two hallmark activities of enzymes
Products: -

?

additional information

?

-

Saccharolobus solfataricus

Q980D1

663210

Substrates: a freestanding proofreading domain is required for protein synthesis quality control in archaea
Products: -

?

additional information

?

-

Saccharolobus solfataricus

-

663210

Substrates: a freestanding proofreading domain is required for protein synthesis quality control in archaea
Products: -

?

additional information

?

-

Saccharolobus solfataricus

Q980D1

663210

Substrates: L-serine is a poor substrate for the wild-type enzyme, the N-terminal enzyme domain is responsible for editing
Products: -

?

additional information

?

-

Saccharolobus solfataricus

-

663210

Substrates: L-serine is a poor substrate for the wild-type enzyme, the N-terminal enzyme domain is responsible for editing
Products: -

?

additional information

?

-

Saccharomyces cerevisiae

-

399

Substrates: the enzyme examines side chain structures of amino acids in 4 recognition steps. For each step the enzyme uses special distinct structures or conformations of the binding cleft
Products: -

?

additional information

?

-

Saccharomyces cerevisiae

-

399

Substrates: specificity with regard to amino acids, discrimination factors
Products: -

?

additional information

?

-

Saccharomyces cerevisiae

-

716387

Substrates: addition of first nucleotide G-1 to tRNAThr1 allows efficient histidylation by histidyl-tRNA synthetase
Products: -

?

additional information

?

-

Saccharomyces cerevisiae

-

727916

Substrates: no activity with L-Val, L-Ala, or L-Cys
Products: -

?

additional information

?

-

Saccharomyces cerevisiae

P07236

745918

Substrates: ScmtThrRS exhibits a tRNA-dependent pre-transfer editing activity that is specific for the tRNAThr2 isoacceptor, whereas tRNAThr1 is unable to stimulate such activity. Editing capability of tRNAThr1 with requirement for the presence of an editing domain
Products: -

?

additional information

?

-

Saccharomyces cerevisiae

-

745918

Substrates: ScmtThrRS exhibits a tRNA-dependent pre-transfer editing activity that is specific for the tRNAThr2 isoacceptor, whereas tRNAThr1 is unable to stimulate such activity. Editing capability of tRNAThr1 with requirement for the presence of an editing domain
Products: -

?

additional information

?

-

Saccharomyces cerevisiae

P07236

745918

Substrates: the enzyme, mitochondrial ThrRS (ScmtThrRS), catalyzes the aminoacylation of tRNAThr1 and tRNAThr2, U33a and G36 of tRNAThr1 are critical nucleotides for aminoacylation by ScmtThrRS. tRNAThr1 stimulates pre-transfer editing in the presence of an editing domain
Products: -

?

additional information

?

-

Saccharomyces cerevisiae

-

745918

Substrates: the enzyme, mitochondrial ThrRS (ScmtThrRS), catalyzes the aminoacylation of tRNAThr1 and tRNAThr2, U33a and G36 of tRNAThr1 are critical nucleotides for aminoacylation by ScmtThrRS. tRNAThr1 stimulates pre-transfer editing in the presence of an editing domain
Products: -

?

additional information

?

-

Saccharomyces cerevisiae

-

743906

Substrates: residues Gln180 and Gln292 are important in cofactor binding
Products: -

?

additional information

?

-

Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

745918

Substrates: ScmtThrRS exhibits a tRNA-dependent pre-transfer editing activity that is specific for the tRNAThr2 isoacceptor, whereas tRNAThr1 is unable to stimulate such activity. Editing capability of tRNAThr1 with requirement for the presence of an editing domain
Products: -

?

additional information

?

-

Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

745918

Substrates: the enzyme, mitochondrial ThrRS (ScmtThrRS), catalyzes the aminoacylation of tRNAThr1 and tRNAThr2, U33a and G36 of tRNAThr1 are critical nucleotides for aminoacylation by ScmtThrRS. tRNAThr1 stimulates pre-transfer editing in the presence of an editing domain
Products: -

?

top print hide show all columns Go to Natural Substrate Search

NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

ATP + L-isoleucine + tRNAIle

AMP + diphosphate + L-isoleucyl-tRNAIle

Oryctolagus cuniculus

-

652046

Substrates: the reaction catalyzed by the enzyme plays an important role in the transport of aminoacylated tRNAs from the nucleus to the cytoplasm
Products: -

?

ATP + L-serine + tRNASer

?

Saccharomyces cerevisiae

-

727916

Substrates: yeast mitochondrial threonyl-tRNA synthetase MST1 lacks an editing domain and utilizes pre-transfer editing to discriminate against serine. MST1 misactivates serine and edits seryl adenylate (Ser-AMP) in the absence of the cognate tRNA. MST1 hydrolyzes 80% of misactivated Ser-AMP at a rate 4fold higher than that for the cognate threonyl adenylate (Thr-AMP) while releasing 20% of Ser-AMP into the solution.
Products: -

?

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

3 entries

ATP + L-threonine + tRNA1Thr

AMP + diphosphate + L-threonyl-tRNA1Thr

Saccharomyces cerevisiae

-

728681

Substrates: -
Products: -

?

ATP + L-threonine + tRNA2Thr

AMP + diphosphate + L-threonyl-tRNA2Thr

Saccharomyces cerevisiae

-

728681

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

?

Thermus thermophilus

-

398

Substrates: catalyzes the attachment of threonine onto its cognate tRNA molecule, prior to participation of the aminoacylated tRNA in the protein synthesis
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

44 entries

ATP + L-threonine + tRNAThr1

AMP + diphosphate + L-threonyl-tRNAThr1

2 entries

ATP + L-threonine + tRNAThr2

AMP + diphosphate + L-threonyl-tRNAThr2

2 entries

additional information

?

-

14 entries

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

Mycoplasma capricolum

-

745349

Substrates: reaction of EC 6.1.1.11, mischarging of tRNAThr
Products: -

?

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

[Mycoplasma] mobile

Q6KH76

745349

Substrates: reaction of EC 6.1.1.11, mischarging of tRNAThr, low activity
Products: -

?

ATP + L-serine + tRNAThr

AMP + diphosphate + L-seryl-tRNAThr

[Mycoplasma] mobile ATCC 43663

Q6KH76

745349

Substrates: reaction of EC 6.1.1.11, mischarging of tRNAThr, low activity
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix

Q9YDW0, Q9YFY3

654016

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix

Q9YDW0, Q9YFY3

705167

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix

-

728382, 726613

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Aeropyrum pernix DSM 11879

-

728382

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Arabidopsis thaliana

-

705818

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Arabidopsis thaliana

F4IFC5

744913

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Archaeoglobus fulgidus

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Brucella abortus

-

745113

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Burkholderia thailandensis

-

728097

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Candida albicans

-

716387

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Cricetulus griseus

-

652046

Substrates: the reaction catalyzed by the enzyme plays an important role in the transport of aminoacylated tRNAs from the nucleus to the cytoplasm
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

650200, 650778, 650784

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

653312

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

653273

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

653324

Substrates: regulatory mechanism
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

663398, 662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

662829

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

714230, 715520, 716771

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

728193

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

728097

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

745920, 744225

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

-

744225

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli

P0A8M3

745349, 745397

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli K-12

-

674339

Substrates: the enzyme acts as both an enzyme and a regulator of gene expression, it aminoacylates tRNAThr isoacceptors and binds to its own mRNA, inhibiting its translation, overview
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Escherichia coli MG1655

-

716771

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Halobacterium sp.

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Haloferax volcanii

-

654016, 728410

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Helicobacter pylori

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Homo sapiens

P26639

728097, 744595

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Homo sapiens

-

744595

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Methanocaldococcus jannaschii

Q58597

663210

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Methanocaldococcus jannaschii

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Methanosarcina mazei

-

663379

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Mycoplasma capricolum

-

745349

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Pyrococcus abyssi

-

660587

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Pyrococcus abyssi

Q9UZ14

673326, 704188

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Saccharolobus solfataricus

Q980D1

663210

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Saccharolobus solfataricus

-

662443

Substrates: -
Products: -

r

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Saccharomyces cerevisiae

-

716387, 727916, 728681, 743906

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Staphylococcus aureus

Q8NW68

652919

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

Yersinia pestis

-

728097

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

[Mycoplasma] mobile

Q6KH76

745349

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr

AMP + diphosphate + L-threonyl-tRNAThr

[Mycoplasma] mobile ATCC 43663

Q6KH76

745349

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr1

AMP + diphosphate + L-threonyl-tRNAThr1

Saccharomyces cerevisiae

P07236

745918

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr1

AMP + diphosphate + L-threonyl-tRNAThr1

Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

745918

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr2

AMP + diphosphate + L-threonyl-tRNAThr2

Saccharomyces cerevisiae

P07236

745918

Substrates: -
Products: -

?

ATP + L-threonine + tRNAThr2

AMP + diphosphate + L-threonyl-tRNAThr2

Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

745918

Substrates: -
Products: -

?

additional information

?

-

Arabidopsis thaliana

F4IFC5

744913

Substrates: interaction of the dual targeting peptide of Thr-tRNA synthetase with the chloroplastic receptor Toc34 in Arabidopsis thaliana as a function of AtToc34 concentration. Mapping the AtToc34 interaction sites in AtThrRS-dTP(2-60), overview
Products: -

?

additional information

?

-

Arabidopsis thaliana

-

744913

Substrates: interaction of the dual targeting peptide of Thr-tRNA synthetase with the chloroplastic receptor Toc34 in Arabidopsis thaliana as a function of AtToc34 concentration. Mapping the AtToc34 interaction sites in AtThrRS-dTP(2-60), overview
Products: -

?

additional information

?

-

Cricetulus griseus

-

652046

Substrates: aminoacyl-tRNA is channeled in vivo by probably direct transfer to elongation factor I
Products: -

?

additional information

?

-

Escherichia coli

-

653273

Substrates: regulation mechanism, 2 essential steps of regulation are operator recognition and inhibition of ribosome binding performed by different domains of the enzyme
Products: -

?

additional information

?

-

Escherichia coli

P0A8M3

662829

Substrates: the enzyme needs to discriminate between threonine, serine, and valine in vivo, mechanism of proofreading of threonyl-tRNA synthetase at atomic resolution, overview
Products: -

?

additional information

?

-

Methanocaldococcus jannaschii

Q58597

663210

Substrates: a freestanding proofreading domain is required for protein synthesis quality control in archaea
Products: -

?

additional information

?

-

Methanosarcina mazei

-

663379

Substrates: structure-based evolutionary considerations
Products: -

?

additional information

?

-

Oryctolagus cuniculus

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652046

Substrates: aminoacyl-tRNA is channeled in vivo by probably direct transfer to elongation factor I
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additional information

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Saccharolobus solfataricus

Q980D1

663210

Substrates: a freestanding proofreading domain is required for protein synthesis quality control in archaea
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additional information

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Saccharolobus solfataricus

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663210

Substrates: a freestanding proofreading domain is required for protein synthesis quality control in archaea
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additional information

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Saccharomyces cerevisiae

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727916

Substrates: no activity with L-Val, L-Ala, or L-Cys
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additional information

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Saccharomyces cerevisiae

P07236

745918

Substrates: ScmtThrRS exhibits a tRNA-dependent pre-transfer editing activity that is specific for the tRNAThr2 isoacceptor, whereas tRNAThr1 is unable to stimulate such activity. Editing capability of tRNAThr1 with requirement for the presence of an editing domain
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additional information

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Saccharomyces cerevisiae

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745918

Substrates: ScmtThrRS exhibits a tRNA-dependent pre-transfer editing activity that is specific for the tRNAThr2 isoacceptor, whereas tRNAThr1 is unable to stimulate such activity. Editing capability of tRNAThr1 with requirement for the presence of an editing domain
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additional information

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Saccharomyces cerevisiae ATCC 204508 / S288c

P07236

745918

Substrates: ScmtThrRS exhibits a tRNA-dependent pre-transfer editing activity that is specific for the tRNAThr2 isoacceptor, whereas tRNAThr1 is unable to stimulate such activity. Editing capability of tRNAThr1 with requirement for the presence of an editing domain
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