Literature summary for 6.1.1.26 extracted from

Uyeda, A.; Watanabe, T.; Kato, Y.; Watanabe, H.; Yomo, T.; Hohsaka, T.; Matsuura, T.
Liposome-based in vitro evolution of aminoacyl-tRNA synthetase for enhanced pyrrolysine derivative incorporation (2015), ChemBioChem, 16, 1797-1802 .

Search for more literature for 6.1.1.26

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of GFP-tagged mutant enzymes	Methanosarcina sp.

Protein Variants

Protein Variants	Comment	Organism
additional information	liposome-based in vitro compartmentalization (IVC) for LysZRS evolution, directed evolution of LysZ-RS, overview	Methanosarcina sp.
Y384F/Y306A	site-directed mutagenesis, the mutant can also incorporate L-lysine derivatives N-benzyloxycarbonyl-L-lysines, i.e. LysZ	Methanosarcina sp.

Organism

Organism	UniProt	Comment	Textmining
Methanosarcina sp.	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	Methanosarcina species pyrrolysyl-tRNA synthetase (PylRS) attaches Pyl to its cognate amber suppressor tRNA. The introduction of two mutations (Y384F and Y306A) into PylRS generates a mutant, designated LysZ-RS, that is able to attach N-benzyloxycarbonyl-L-lysine (LysZ) to its cognate tRNA	Methanosarcina sp.	?	-	?

Synonyms

Synonyms	Comment	Organism
LysZ-RS	-	Methanosarcina sp.
PylRS	-	Methanosarcina sp.
pyrrolysyl-tRNA synthetase	-	Methanosarcina sp.

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Release 2023.1 (January 2023)