Organism | UniProt | Comment | Textmining |
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Methanosarcina acetivorans | - |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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ATP + L-pyrrolysine + tRNAPyl | the Methanosarcina acetivorans tRNAhis guanylyltranferase Thg1 gene contains an in-frame TAG codon. Its presence in Methanosarcina mRNA may lead to pyrrolysine incorporation achieved by Pyl-tRNAPyl, the product of pyrrolysyl-tRNA synthetase. Translation of Thg1 mRNA leads to a full-length, Pyl-containing, active enzyme as determined by immunoblotting, mass spectrometry, and biochemical analysis | Methanosarcina acetivorans | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
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General Information | Comment | Organism |
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physiological function | pyrrolysine insertion is akin to natural suppression and unlike the active stop codon reassignment that is required for selenocysteine insertion. In Thg1, pyrrolysine is a dispensable residue that appears to confer no selective advantage | Methanosarcina acetivorans |