Literature summary for 5.6.2.2 extracted from

Gadelle, D.; Krupovic, M.; Raymann, K.; Mayer, C.; Forterre, P.
DNA topoisomerase VIII A novel subfamily of type IIB topoisomerases encoded by free or integrated plasmids in Archaea and Bacteria (2014), Nucleic Acids Res., 42, 8578-8591 .

Search for more literature for 5.6.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Paenibacillus polymyxa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on	Paenibacillus polymyxa

Organism

Organism	UniProt	Comment	Textmining
Paenibacillus polymyxa	-	-	-
Paenibacillus polymyxa M1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Strep-tactin column chromatography and Superdex 200 gel filtration	Paenibacillus polymyxa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
catenated kDNA + ATP + H2O	very weak decatenation activity	Paenibacillus polymyxa	decatenated kDNA + ADP + phosphate	-	?
catenated kDNA + ATP + H2O	very weak decatenation activity	Paenibacillus polymyxa M1	decatenated kDNA + ADP + phosphate	-	?
negatively supercoiled plasmid pBR322 DNA + ATP + H2O	-	Paenibacillus polymyxa	relaxed plasmid pBR322 DNA + ADP + phosphate	-	?
negatively supercoiled plasmid pBR322 DNA + ATP + H2O	-	Paenibacillus polymyxa M1	relaxed plasmid pBR322 DNA + ADP + phosphate	-	?
positively supercoiled plasmid pBR322 DNA + ATP + H2O	-	Paenibacillus polymyxa	relaxed plasmid pBR322 DNA + ADP + phosphate	-	?
positively supercoiled plasmid pBR322 DNA + ATP + H2O	-	Paenibacillus polymyxa M1	relaxed plasmid pBR322 DNA + ADP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
DNA topoisomerase VIII	-	Paenibacillus polymyxa
type IIB topoisomerase	-	Paenibacillus polymyxa

Cofactor

Cofactor	Comment	Organism	Structure
ATP	dependent on	Paenibacillus polymyxa

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Release 2023.1 (January 2023)