Inhibitors | Comment | Organism | Structure |
---|---|---|---|
etoposide | etoposide triggers the formation of topoisomerase II-DNA adducts. Enzyme-associated DNA breaks can be stabilised by drugs such as etoposide. The proteasome has a role in converting etoposide-stabilised protein-DNA complexes into frank double-strand breaks | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of formation and removal of topoisomerase II-DNA complexes in response to ionising radiation or etoposide treatment, overview | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Mus musculus | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | topoisomerase II is an enzyme that alters the topological state of DNA via a transient covalent enzyme-bridged double strand break in the DNA, through which a second DNA helix can pass | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
two isoforms of topoisomerase II, termed alpha and beta | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryonic fibroblast cell line | - |
Mus musculus | - |
mTOP2beta-4 cell | wild-type mouse embryonic fibroblasts | Mus musculus | - |
mtop2beta-5 cell | immortalized mouse fibroblasts lacking topoisomerase IIbeta | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | topoisomerase II is an enzyme that alters the topological state of DNA via a transient covalent enzyme-bridged double strand break in the DNA, through which a second DNA helix can pass | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
topoisomerase IIalpha | - |
Mus musculus |
topoisomerase IIbeta | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
additional information | ionising radiation does not influence enzyme-DNA complexes and does not produce topoisomerase II-DNA adducts, overview. Topoisomerase II-DNA complexes inwild-type cells but not in TOP2beta-/- cells increase significantly at 24 hours with the proteasome inhibitor MG132, suggesting topoisomerase IIbeta adducts are removed by the proteasome | Mus musculus |