Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Lim15/Dmc1 | from Coprimus cinereus, a meiosis-specific RecA-like protein, activates the DNA relaxation and decatenation activity of topoisomerase II in vitro, the enzyme suppresses Lim15/Dmc1-dependent strand transfer activity and activates its DNA-dependent ATPase activity in vitro, overview | Coprinopsis cinerea |
Cloned (Comment) | Organism |
---|---|
expression analysis of enzyme and protein Lim15 in vivo during meiosis, expression of Lim15 and topoisomerase II in the yeast two-hybrid system using Saccharomyces cerevisiae strain AH109, expression of His-tagged enzyme comprising residues 1066-1569 in Escherichia coli | Coprinopsis cinerea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Lim15/Dmc1 | from Coprimus cinereus, a meiosis-specific RecA-like protein, Lim15 inhibits the topoisomerase II activity in vivo, but activates the DNA relaxation and decatenation activity of topoisomerase II in vitro | Coprinopsis cinerea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromosome | co-localization with meiosis-specific RecA-like protein Lim15/Dmc1 from the leptotene to the zygotene stage | Coprinopsis cinerea | 5694 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for ATPase activity | Coprinopsis cinerea | |
Mg2+ | - |
Coprinopsis cinerea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Coprinopsis cinerea | DNA topoisomerase II interacts with Lim15/Dmc1 in meiosis, the enzyme suppresses Lim15/Dmc1-dependent strand transfer activity and activates its DNA-dependent ATPase activity in vitro, overview, Lim15 inhibits the topoisomerase II activity in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | Q5KSK9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme comprising residues 1066-1569 from Escherichia coli by nickel affinity chromatography | Coprinopsis cinerea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | expression analysis of enzyme and protein Lim15 in vivo during meiosis, overview | Coprinopsis cinerea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded M13 DNA + ATP + H2O | breakage, passage, and rejoining of double-stranded M13 DNA, including ATPase activity | Coprinopsis cinerea | ? | - |
? | |
additional information | DNA topoisomerase II interacts with Lim15/Dmc1 in meiosis, the enzyme suppresses Lim15/Dmc1-dependent strand transfer activity and activates its DNA-dependent ATPase activity in vitro, overview, Lim15 inhibits the topoisomerase II activity in vivo | Coprinopsis cinerea | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA topoisomerase II | - |
Coprinopsis cinerea |
TopII | - |
Coprinopsis cinerea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Coprinopsis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Coprinopsis cinerea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Coprinopsis cinerea |