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Literature summary for 5.6.2.2 extracted from

  • Austin, C.A.; Marsh, K.L.; Wasserman, R.A.; Willmore, E.; Sayer, P.J.; Wang, J.C.; Fisher, L.M.
    Expression, domain structure, and enzymatic properties of an active recombinant human DNA topoisomerase IIbeta (1995), J. Biol. Chem., 270, 15739-15746.
    View publication on PubMed
Search for more literature for 5.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
topoisomerase IIbeta overexpressed in Saccharomyces cerevisiae Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
175000
-
 x * 175000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
topoisomerase IIbeta Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
network of DNA rings + ATP + H2O decatenation Homo sapiens monomeric DNA circles + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O catenation Homo sapiens catenated DNA networks + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O relaxation Homo sapiens relaxed DNA + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O ATP-dependent relaxation of supercoiled pBR322 DNA Homo sapiens relaxed DNA + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 175000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 25 h, stable Homo sapiens
25
-
 24 h, stable Homo sapiens
37
-
 activity begins to decrease after 8 h incubation Homo sapiens