KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ATP | relaxation reaction | Bos taurus | |
0.5 | - |
dATP | relaxation reaction | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Bos taurus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential cofactor for the reaction at concentrations between 0.5-8 mM, optimal concentration: 4 mM | Bos taurus | |
Mn2+ | can substitute for Mg2+, but only at concentrations between 0.2 and 0.4 mM | Bos taurus | |
NaCl | catenation and relaxation exhibit a salt optimum at 130 mM. At concentrations below 30 mM and above 200 mM the enzyme is inactive | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
1 * 150000 + 1 * 175000, SDS-PAGE | Bos taurus |
175000 | - |
1 * 150000 + 1 * 175000, SDS-PAGE | Bos taurus |
300000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, 50% v/v glycerol, stable for at least 12 months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + negatively supercoiled circular DNA | - |
Bos taurus | dADP + phosphate + relaxed circular DNA | - |
? | |
additional information | - |
Bos taurus | ? | - |
? | |
network of DNA rings + ATP + H2O | decatenation | Bos taurus | monomeric DNA circles + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | catenation | Bos taurus | catenated DNA networks + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | in presence of condensing agents like histone H1 or spermidine | Bos taurus | catenated DNA networks + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | relaxation | Bos taurus | relaxed DNA + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | relaxation of negatively or positively supercoiled DNA | Bos taurus | relaxed DNA + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | dATP can substitute for ATP | Bos taurus | relaxed DNA + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 150000 + 1 * 175000, SDS-PAGE | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | - |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7: about 55% of maximal activity, pH 10: about 25% of maximal activity, relaxing reaction | Bos taurus |