Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Streptomyces peucetius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activity is not accelerated or significantly inhibited by divalent metal ions (Zn2+, Co2+, Fe2+, Cu2+, or Mn2+) or chelating reagents | Streptomyces peucetius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0521 | - |
methyl aklanonate | pH 7.0, 30°C | Streptomyces peucetius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activity is not accelerated or significantly inhibited by divalent metal ions (Zn2+, Co2+, Fe2+, Cu2+, or Mn2+) or chelating reagents | Streptomyces peucetius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
x * 16000, SDS-PAGE | Streptomyces peucetius |
16572 | - |
x * 16572, electrospray MS analysis | Streptomyces peucetius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl aklanonate | Streptomyces peucetius | - |
aklaviketone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces peucetius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces peucetius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.3 | - |
pH 7.0, 30°C | Streptomyces peucetius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl aklanonate | - |
Streptomyces peucetius | aklaviketone | - |
? | |
methyl aklanonate | base-catalyzed mechanism | Streptomyces peucetius | aklaviketone | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 16000, SDS-PAGE | Streptomyces peucetius |
? | x * 16572, electrospray MS analysis | Streptomyces peucetius |
Synonyms | Comment | Organism |
---|---|---|
AAMEcyclase | - |
Streptomyces peucetius |
DnrD | - |
Streptomyces peucetius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces peucetius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptomyces peucetius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH 6.5: about 65% of maximal activity, 9.0: about 30% of maximal activity | Streptomyces peucetius |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in biosynthesis of aklaviketone, an intermediate in the biosynthetic pathway to daunorubicin and doxorubicin | Streptomyces peucetius |