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show all sequences of 5.4.99.40

Molecular cloning and functional expression of triterpene synthases from pea (Pisum sativum) new alpha-amyrin-producing enzyme is a multifunctional triterpene synthase

Morita, M.; Shibuya, M.; Kushiro, T.; Masuda, K.; Ebizuka, Y.; Eur. J. Biochem. 267, 3453-3460 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of PSM in ERG7 deficient yeast mutant GIL77
Pisum sativum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
Pisum sativum
PSM produces both alpha- and beta-amyrin in about 3 : 2 ratio together with at least six other minor triterpenes
alpha-amyrin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pisum sativum
Q9LRH7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
PSM produces both alpha- and beta-amyrin in about 3 : 2 ratio together with at least six other minor triterpenes
683422
Pisum sativum
alpha-amyrin
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of PSM in ERG7 deficient yeast mutant GIL77
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
Pisum sativum
PSM produces both alpha- and beta-amyrin in about 3 : 2 ratio together with at least six other minor triterpenes
alpha-amyrin
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
PSM produces both alpha- and beta-amyrin in about 3 : 2 ratio together with at least six other minor triterpenes
683422
Pisum sativum
alpha-amyrin
-
-
-
?
Other publictions for EC 5.4.99.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730640
Misra
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164
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3
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1
1
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730557
Vishwakarma
Molecular characterization and ...
Bacopa monnieri
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19
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1
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1
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5
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1
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1
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711990
Brendolise
An unusual plant triterpene sy ...
Malus domestica
FEBS J.
278
2485-2499
2011
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1
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7
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2
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716616
Wang
Two oxidosqualene cyclases res ...
Solanum lycopersicum
Plant Physiol.
155
540-552
2011
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1
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3
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1
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3
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683422
Morita
Molecular cloning and function ...
Pisum sativum
Eur. J. Biochem.
267
3453-3460
2000
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1
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1
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4
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1
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1
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