Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Acetoanaerobium sticklandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | purified KamDE contains a substantial quantity of metal ions, primarily Zn2+ | Acetoanaerobium sticklandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29191 | - |
alpha2,beta2, 2 * 57261 + 2 * 29191, deduced from nucleotide sequence | Acetoanaerobium sticklandii |
30000 | - |
alpha2,beta2, 2 * 55000 + 2 * 30000, SDS-PAGE | Acetoanaerobium sticklandii |
55000 | - |
alpha2,beta2, 2 * 55000 + 2 * 30000, SDS-PAGE | Acetoanaerobium sticklandii |
57261 | - |
alpha2,beta2, 2 * 57261 + 2 * 29191, deduced from nucleotide sequence | Acetoanaerobium sticklandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | Acetoanaerobium sticklandii | first step in D-lysine catabolism | 2,5-diaminohexanoate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant and native KamDE | Acetoanaerobium sticklandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.95 | - |
- |
Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | - |
Acetoanaerobium sticklandii | 2,5-diaminohexanoate | - |
r | |
L-lysine | first step in D-lysine catabolism | Acetoanaerobium sticklandii | 2,5-diaminohexanoate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2,beta2, 2 * 55000 + 2 * 30000, SDS-PAGE | Acetoanaerobium sticklandii |
tetramer | alpha2,beta2, 2 * 57261 + 2 * 29191, deduced from nucleotide sequence | Acetoanaerobium sticklandii |
Synonyms | Comment | Organism |
---|---|---|
KamDE | - |
Acetoanaerobium sticklandii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | approx. 50% loss of activity at 43°C after 5 min, almost complete loss of activity at 55°C after 5 min | Acetoanaerobium sticklandii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adenosylcobalamine | - |
Acetoanaerobium sticklandii | |
pyridoxal 5'-phosphate | - |
Acetoanaerobium sticklandii |