Literature summary for 5.3.1.5 extracted from

Vilonen, K.M.; Vuolanto, A.; Jokela, J.; Leisola, M.S.; Krause, A.O.
Enhanced glucose to fructose conversion in acetone with xylose isomerase stabilized by crystallization and cross-linking (2004), Biotechnol. Prog., 20, 1555-1560.

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Application

Application	Comment	Organism
nutrition	high fructose corn syrups	Streptomyces rubiginosus

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Streptomyces rubiginosus

Organic Solvent Stability

Organic Solvent	Comment	Organism
Acetone	70-80% of cross-linked crystalline xylose isomerase activity is left after incubation for 24 h at 50°C in buffer solutions (pH 7.2) containing 10-90% acetone. Soluble xylose isomerase is considerably less stable in acetone-containing solutions. The addition of acetone enhances the production of fructose from glucose by enhancing the reaction rate and shifting the equilibrium toward fructose. However, xylose isomerase must be in the form of cross-linked crystals for maximal activity and stability	Streptomyces rubiginosus
Ethanol	in buffer containing 50% ethanol only 2% of the initial cross-linked crystalline xylose isomerase activity is retained after 24 h at 50°C. Soluble xylose isomerase is considerably less stable in ethanol-containing solutions	Streptomyces rubiginosus
Methanol	cross-linked crystalline xylose isomerase is inactivated in 24 h at 50°C even more in maleate buffer containing 50% methanol	Streptomyces rubiginosus
Pyridine	cross-linked crystalline xylose isomerase is inactivated in 24 h at 50°C even more in maleate buffer containing 10% pyridine	Streptomyces rubiginosus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces rubiginosus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Glucose	-	Streptomyces rubiginosus	D-Fructose	-	r

Synonyms

Synonyms	Comment	Organism
glucose isomerase	-	Streptomyces rubiginosus
XI	-	Streptomyces rubiginosus
xylose isomerase	-	Streptomyces rubiginosus

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Release 2023.1 (January 2023)