Crystallization (Comment) | Organism |
---|---|
- |
Streptomyces olivochromogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Geobacillus stearothermophilus | |
Cu2+ | - |
Streptomyces olivochromogenes | |
Mn2+ | - |
Streptomyces olivochromogenes | |
Ni2+ | - |
Geobacillus stearothermophilus | |
Ni2+ | - |
Streptomyces olivochromogenes | |
Sn2+ | - |
Geobacillus stearothermophilus | |
Zn2+ | - |
Geobacillus stearothermophilus | |
Zn2+ | - |
Streptomyces olivochromogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
xylulose | - |
Streptomyces olivochromogenes | |
33 | - |
D-xylose | - |
Streptomyces olivochromogenes | |
100 | - |
D-xylulose | - |
Geobacillus stearothermophilus | |
200 | - |
D-fructose | - |
Geobacillus stearothermophilus | |
200 | - |
D-fructose | - |
Streptomyces olivochromogenes | |
220 | - |
D-fructose | - |
Streptomyces olivochromogenes | |
250 | - |
D-glucose | - |
Streptomyces olivochromogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, SDS-PAGE | Streptomyces olivochromogenes |
115000 | 117000 | low speed sedimentation without reaching equilibrium, equilibrium sedimentation | Streptomyces olivochromogenes |
127000 | - |
equilibrium sedimentation | Geobacillus stearothermophilus |
130000 | - |
1 * 130000, SDS-PAGE | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Streptomyces olivochromogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
- |
Streptomyces olivochromogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
- |
Streptomyces olivochromogenes |
2.7 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Fructose | - |
Geobacillus stearothermophilus | ? | - |
? | |
D-Fructose | - |
Streptomyces olivochromogenes | ? | - |
? | |
D-Glucose | - |
Geobacillus stearothermophilus | D-Fructose | - |
? | |
D-Glucose | - |
Streptomyces olivochromogenes | D-Fructose | - |
? | |
D-Xylose | - |
Geobacillus stearothermophilus | D-Xylulose | - |
? | |
D-Xylose | r | Streptomyces olivochromogenes | D-Xylulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 56000, SDS-PAGE | Streptomyces olivochromogenes |
monomer | 1 * 130000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Geobacillus stearothermophilus |
80 | - |
- |
Streptomyces olivochromogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, stable | Streptomyces olivochromogenes |
65 | - |
30 min, about 50% loss of activity | Streptomyces olivochromogenes |
70 | - |
- |
Geobacillus stearothermophilus |
70 | - |
30 min, about 70% loss of activity | Streptomyces olivochromogenes |
75 | - |
60 min, stable up to | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Geobacillus stearothermophilus |
8 | 10 | - |
Streptomyces olivochromogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6: about 35% of maximal activity, pH 10: about 30% of maximal activity | Streptomyces olivochromogenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
room temperature, 30 min, stable above | Streptomyces olivochromogenes |
5 | - |
room temperature, 30 min, stable above | Geobacillus stearothermophilus |