Crystallization (Comment) | Organism |
---|---|
structures of wild-type and mutants at residues T75 and Q64 | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
Q64E | mutation weakens the dimeric structure | Plasmodium falciparum |
Q64N | mutation weakens the dimeric structure | Plasmodium falciparum |
T75C | activity similar to wild-type, dimer integrity is unimpaired. Decrease in stability between 35°C and 45°C | Plasmodium falciparum |
T75N | 4fold drop in activity compared to wild-type. Decrease in stability between 35°C and 45°C | Plasmodium falciparum |
T75S | activity similar to wild-type, dimer integrity is unimpaired. Decrease in stability between 35°C and 45°C | Plasmodium falciparum |
T75V | activity similar to wild-type, dimer integrity is unimpaired. Decrease in stability between 35°C and 45°C | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.6, 23°C | Plasmodium falciparum | |
0.8 | - |
D-glyceraldehyde 3-phosphate | mutant T75S, pH 7.6, 23°C | Plasmodium falciparum | |
0.86 | - |
D-glyceraldehyde 3-phosphate | mutant T75N, pH 7.6, 23°C | Plasmodium falciparum | |
0.91 | - |
D-glyceraldehyde 3-phosphate | mutant T75V, pH 7.6, 23°C | Plasmodium falciparum | |
1.15 | - |
D-glyceraldehyde 3-phosphate | mutant T75C, pH 7.6, 23°C | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q07412 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63.3 | - |
mutant Q64E, pH 7.6, 23°C | Plasmodium falciparum |
134 | - |
mutant Q64N, pH 7.6, 23°C | Plasmodium falciparum |
679 | - |
wild-type, pH 7.6, 23°C | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Plasmodium falciparum | dihydroxyacetone 3-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
D-glyceraldehyde 3-phosphate | mutant T75N, pH 7.6, 23°C | Plasmodium falciparum | |
0.034 | - |
D-glyceraldehyde 3-phosphate | mutant T75C, pH 7.6, 23°C | Plasmodium falciparum | |
0.038 | - |
D-glyceraldehyde 3-phosphate | mutant T75S, pH 7.6, 23°C | Plasmodium falciparum | |
0.038 | - |
D-glyceraldehyde 3-phosphate | mutant T75V, pH 7.6, 23°C | Plasmodium falciparum | |
0.04 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.6, 23°C | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
151 | - |
D-glyceraldehyde 3-phosphate | mutant T75N, pH 7.6, 23°C | Plasmodium falciparum | |
296 | - |
D-glyceraldehyde 3-phosphate | mutant T75C, pH 7.6, 23°C | Plasmodium falciparum | |
418 | - |
D-glyceraldehyde 3-phosphate | mutant T75V, pH 7.6, 23°C | Plasmodium falciparum | |
475 | - |
D-glyceraldehyde 3-phosphate | mutant T75S, pH 7.6, 23°C | Plasmodium falciparum | |
645 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.6, 23°C | Plasmodium falciparum |