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D-glyceraldehyde 3-phosphate
dihydroxyacetone 3-phosphate
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
dihydroxyacetone 3-phosphate
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
D-glyceraldehyde 3-phosphate
glycerone phosphate
D-glyceraldehyde 3-phosphate
glycerone phosphate
methylglyoxal + phosphate
-
-
-
?
additional information
?
-
D-glyceraldehyde 3-phosphate

dihydroxyacetone 3-phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone 3-phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone 3-phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone 3-phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone 3-phosphate
-
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone 3-phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone 3-phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone 3-phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone 3-phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone 3-phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate

dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
isomerization of D-glyceraldehyde 3-phosphate in D2O proceeds with 49% intramolecular transfer of the 1 H label from substrate to product dihydroxyacetone phosphate
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
the active site of free enzyme, which has an open conformation needed to allow substrate binding, adopts a closed conformation at the enediolate-complex intermediate where the catalytic side chain is sequestered from interaction with imidazole dissolved in D2O
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
cytoplasmic triosephosphate isomerase may be important in the glycolytic pathway for the supply of C to respiratory and biosynthetic pathways during active growth
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
r
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
-
-
-
-
?
D-Glyceraldehyde 3-phosphate

Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
stereospecific reaction via an enzyme-bound enediol(ate) intermediate, proton transfer from D-glyceraldehyde 3-phosphate to the carboxylate side chain of TIM Glu165, irreversible labeling with deuterium , overview
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
r
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
r
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
r
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
the reaction is essential in vivo
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
triosephosphate isomerase is an enolizing enzyme, which catalyses the interconversion of dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
TIM is highly specific for the substrates
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
r
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
r
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
substrate binding perturbs residues Asn10, His95, Ser100, Glu129-Glu133, Val167-Ala186, Asn213, and Leu230-Leu126 that either are near the ligand or have direct contact with the ligand
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
key reaction in glycolysis
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
r
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
r
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
r
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
-
r
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
-
-
-
?
dihydroxyacetone 3-phosphate

D-glyceraldehyde 3-phosphate
-
-
-
-
r
dihydroxyacetone 3-phosphate
D-glyceraldehyde 3-phosphate
-
-
-
?
dihydroxyacetone phosphate

D-glyceraldehyde 3-phosphate
-
-
-
r
dihydroxyacetone phosphate
D-glyceraldehyde 3-phosphate
-
-
-
-
r
dihydroxyacetone phosphate
D-glyceraldehyde 3-phosphate
-
-
-
?
dihydroxyacetone phosphate
D-glyceraldehyde 3-phosphate
-
-
-
?
dihydroxyacetone phosphate
D-glyceraldehyde 3-phosphate
-
-
-
r
dihydroxyacetone phosphate
D-glyceraldehyde 3-phosphate
-
-
-
r
dihydroxyacetone phosphate
D-glyceraldehyde 3-phosphate
-
-
-
-
r
glycerone phosphate

D-glyceraldehyde 3-phosphate
-
-
-
?
glycerone phosphate
D-glyceraldehyde 3-phosphate
-
-
-
-
?
glycerone phosphate
D-glyceraldehyde 3-phosphate
-
-
-
-
?
glycerone phosphate
D-glyceraldehyde 3-phosphate
-
-
-
?
glycerone phosphate
D-glyceraldehyde 3-phosphate
-
-
-
?
glycolaldehyde

?
-
-
-
-
?
glycolaldehyde
?
-
TIM-catalyzed reactions of glycolaldehyde are activated by phosphite dianion, kinetics, overview
-
-
?
glycolaldehyde
?
-
-
-
-
?
additional information

?
-
enzyme is confirmed by total proteome analysis of glycerol-grown cells
-
-
?
additional information
?
-
-
enzyme may be involved in integrin alphaIIb/beta3-mediated platelet function
-
-
?
additional information
?
-
the proton transfer reaction from PGH to the Glu167 side chain, when PGH becomes sequestered in the active site, active site modeling, overview
-
-
?
additional information
?
-
-
Tau, main component of the aberrant paired helical filaments found in Alzheimer's disease, interacts with the enzyme and protects TPI against oxidative damage, overview. Interaction between Tau and triose phosphate isomerase occurs in a normal, nondisease state as well as in a neurodegeneration state
-
-
?
additional information
?
-
-
formation of a two-part substrate by carving up D-glyceraldehyde 3-phosphate into the minimal neutral two-carbon sugar glycolaldehyde and phosphite dianion pieces. Enzyme catalyzes proton transfer from glycolaldehyde in D2O with a ratio of kcat to Km of 0.26 per M and s. Addition of exogenous phosphite dianion results in a large increase in the observed second-order rate constant for turnover of glycolaldehyde. Binding of phosphite dianion to the free enzyme is 700fold weaker than its binding to the fleeting complex of the enzyme with the altered substrate in the transition state
-
-
?
additional information
?
-
importance of conserved residues in the vicinity of the active site that serve to position the functional K12 residue. A network of key interactions spans the interacting subunits
-
-
?
additional information
?
-
-
importance of conserved residues in the vicinity of the active site that serve to position the functional K12 residue. A network of key interactions spans the interacting subunits
-
-
?
additional information
?
-
Plasmodium falciparum TIM is unique in possessing a Phe residue at position 96 in place of the conserved Ser that is found in TIMs from the majority of other organisms
-
-
?
additional information
?
-
-
Plasmodium falciparum TIM is unique in possessing a Phe residue at position 96 in place of the conserved Ser that is found in TIMs from the majority of other organisms
-
-
?
additional information
?
-
enzyme is involved in arsenate reduction
-
-
?
additional information
?
-
-
enzyme is involved in arsenate reduction
-
-
?
additional information
?
-
the effect of bound phosphite dianion on the activation barrier is small, in comparison to the much larger intrinsic phosphodianion and phosphite dianion binding energy utilized to stabilize the transition states for TIM-catalyzed deprotonation of glyceraldehyde-3-phosphate and glyceraldehyde-phosphite dianion complex, respectively
-
-
?
additional information
?
-
the substrate is locked in a protein cage with the phosphodianion occluded from interaction with solvent water, and ion-paired to the surface alkyl ammonium side chain of residue K12. The neutral imidazole side chain of H95 interacts with the carbonyl oxygen of dihydroxyacetone phosphate and glyceraldehyde 3-phosphate
-
-
?
additional information
?
-
the substrate is locked in a protein cage with the phosphodianion occluded from interaction with solvent water, and ion-paired to the surface alkyl ammonium side chain of residue K12. The neutral imidazole side chain of H95 interacts with the carbonyl oxygen of dihydroxyacetone phosphate and glyceraldehyde 3-phosphate
-
-
?
additional information
?
-
the effect of bound phosphite dianion on the activation barrier is small, in comparison to the much larger intrinsic phosphodianion and phosphite dianion binding energy utilized to stabilize the transition states for TIM-catalyzed deprotonation of glyceraldehyde-3-phosphate and glyceraldehyde-phosphite dianion complex, respectively
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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(1E,3E,6E,8E)-1,9-di(furan-2-yl)nona-1,3,6,8-tetraen-5-one
-
compound binds to the dimer interface and is unable to inactivate Trypanosoma brucei TIM or Homo sapiens TIM at concentrations higher than 100 microM. Compound also affects cruzipain
(1Z,2Z)-N,N'-dihydroxy-4-methylcyclohexa-3,5-diene-1,2-diimine
-
-
(2E)-2-[(4-methyl-5-oxido-1,2,5-oxadiazol-3-yl)methylidene]hydrazinecarbothioamide
-
-
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazinecarbothioamide
-
irreversible inhibitor
(2E)-2-[2-[(3-oxido-2,1,3-benzoxadiazol-5-yl)methoxy]benzylidene]-N-(prop-2-en-1-yl)hydrazinecarbothioamide
-
irreversible inhibitor
(2E)-N-(naphthalen-2-yl)-2-[(2E)-3-(5-nitrofuran-2-yl)prop-2-en-1-ylidene]hydrazinecarboxamide
-
irreversible inhibitor
(2E)-N-[2-(3,4-dimethoxyphenyl)ethyl]-2-[(5-nitrofuran-2-yl)methylidene]hydrazinecarboxamide
-
irreversible inhibitor
(2E,5E)-2,5-bis[(2E)-3-(thiophen-2-yl)prop-2-en-1-ylidene]cyclopentan-1-one
-
compound is unable to inactivate Trypanosoma brucei TIM or Homo sapiens TIM at concentrations higher than 100 microM. Compound also affects cruzipain
(2E,6E)-2,6-bis[(2E)-3-(furan-2-yl)prop-2-en-1-ylidene]cyclohexan-1-one
-
compound binds to the dimer interface and is unable to inactivate Trypanosoma brucei TIM or Homo sapiens TIM at concentrations higher than 100 microM. Compound also affects cruzipain
(4-methyl-1,2,3-thiadiazol-5-yl)methanol
-
29% inhibition at 0.4 mM
1,2,6-thiadiazine
-
irreversible inhibitor
1-Chloro-3-hydroxyacetone
-
-
2,2'-methylenebis(1,3-benzothiazole)
2,6-dibenzyl-4-[(5-nitrothiophen-2-yl)methylidene]-1,2,6-thiadiazinane-3,5-dione 1,1-dioxide
-
irreversible inhibitor
2,9-dimethyl-beta-carbolinium ion
-
-
2-(N-formyl-N-hydroxy)-aminoethyl phosphonate
-
2-carboxyethyl methanethiosulfonate
modifies four Cys per subunit of dimeric protein and induces 97% of inactivation. Inactivation does not affect secondary structure nor induce dimer dissociation. Cys modification decreases thermal stability of the enzyme
2-carboxyethylphosphonic acid
-
-
2-methyl-beta-carbolinium ion
-
-
2-phenyl-4H-chromen-4-one
-
-
2-phosphoglycolic acid
-
-
2-[(1E)-2-nitroprop-1-en-1-yl]thiophene
-
-
2-[(3-aminophenyl)disulfanyl]aniline
-
-
3,5-diphenyl-1,2,4-thiadiazole
-
74% inhibition at 0.4 mM
3-(2-benzothiazolylthio)-1-propanesulfonic acid
binds to the dimer interface of the enzyme and thereby abolishes its function with a high level of selectivity
3-(4-methylphenyl)-5-[(4-methylphenyl)sulfonyl]-1,2,4-thiadiazole
-
-
3-nitrobiphenyl-4-amine
-
-
4-(4-nitrobenzylidene)-2,6-bis(2-phenylethyl)-1,2,6-thiadiazinane-3,5-dione 1,1-dioxide
-
irreversible inhibitor, 85% inhibition at 0.4 mM
4-[(5-nitrofuran-2-yl)methylidene]-4H-1,2,6-thiadiazine-3,5-diamine 1,1-dioxide
-
irreversible inhibitor
4-[(5-nitrothiophen-2-yl)methylidene]-2,6-bis(2-phenylethyl)-1,2,6-thiadiazinane-3,5-dione 1,1-dioxide
-
irreversible inhibitor
5,5'-dithio-bis(2-nitrobenzoic acid)
5,5'-dithiobis(2-nitrobenzoate)
5-[(1E)-2-nitroprop-1-en-1-yl]-1,3-benzodioxole
-
-
6,6'-bi-1,3-benzothiazole-2,2'-diamine
6-phosphogluconate
-
5 mM, 9% inhibition
6-[(E)-2-(5-nitrothiophen-2-yl)ethenyl]-2,1,3-benzoxadiazole 1-oxide
-
irreversible inhibitor
6-[(E)-2-[(4-fluorophenyl)sulfanyl]ethenyl]-2,1,3-benzoxadiazole 1-oxide
-
irreversible inhibitor
8-bromo-5,10-dioxidophenazin-2-yl chloroacetate
-
50% inhibition at 0.4 mM
Atrazine
-
a herbicide which interferes with photosynthesis
bromohydroxyacetone phosphate
butylbrevifolin carboxylate
-
-
Chloroacetol phosphate
-
-
citrate
-
25 mM, 9% inhibition
cyclo(Gly-Pro-Phe-Val-Phe-PSI[CS-NH]Phe)
cyclo[Trp-Phe-D-Pro-Phe-Phe-Lys(benzyloxycarbonyl)-]
cyclo[Trp-Tyr(OSO3Na)-D-Phe-Thr(OSO3Na)-Lys(benzyloxycarbonyl)-]
-
noncompetitive, reversible
D-erythrose 4-phosphate
-
-
Diamide
1 mM, presence of glutathione, complete inhibition by S-glutathionylation
ethyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)-1,2,4-thiadiazole-5-carboxylate
-
-
ethyl 3-phenyl-1,2,4-thiadiazole-5-carboxylate
-
-
ethylbrevifolin carboxylate
-
-
fluorodifen
-
a herbicide which interferes with photosynthesis
fructose 1,6-diphosphate
-
5 mM, 69% inhibition
fructose 6-phosphate
-
5 mM, 44% inhibition
glucose 1-phosphate
-
5 mM, 10% inhibition
glucose 6-phosphate
-
5 mM, 25% inhibition
glutathione disulfide
modification of residues Cys127 and Cys218
GSSG
-
oxidized glutathione is a strong inhibitor of the enzyme at low concentrations
KFGNGSYTGEVS
peptide that corresponds to loop 3 of the triosephosphate isomerase, residues 68-79. Efficient inhibitor with the activity falling to about 45% at 1000fold molar excess of the peptide in case of the wild-type enzyme. In the case of either of the mutants, Y74C and Y74G, even at 100fold molar excess of the peptide, only 30% activity can be obtained
KYGNGSCTGEVS
peptide that is an analog of the peptide that corresponds to loop 3 of the protein, residues 68-79, with the replacement Y74C and F69Y. Inhibits the activity of the mutant enzymes Y74C and Y74G, with about 40% activity remaining in the presence of 1000fold molar excess of the peptide
Mannotriose
-
docking simulation
methyl methanethiosulfonate
methylbrevifolin carboxylate
-
molecular docking simulations and enzyme binding structure, and inhibition kinetics, overview
methylmethane thiosulfonate
N-[(2-oxido-4-phenyl-1,2,5-oxadiazol-3-yl)methyl]naphthalen-1-amine
-
72% inhibition at 0.4 mM
N-[(4-methyl-5-oxido-1,2,5-oxadiazol-3-yl)methyl]naphthalen-1-amine
-
41% inhibition at 0.4 mM
phenazine 5,9-dioxide
-
84% inhibition at 0.4 mM
phosphoglycoloaldoxime
-
-
phosphoglycolohydrazide
-
-
Phosphoglycolohydroxamate
S-Phenyl-p-toluenethiosulfonate
succinate
-
5 mM, 9% inhibition
[3-amino-3-(hydroxyimino)propyl]phosphonic acid
-
-
1,2,4-thiadiazole

-
59% inhibition at 0.1 mM
2,2'-methylenebis(1,3-benzothiazole)

-
irreversible inactivation
2,2'-methylenebis(1,3-benzothiazole)
-
irreversible inactivation
2,2'-methylenebis(1,3-benzothiazole)
-
40% inactivation at 0.05 mM, irreversible
2,4-dinitrofluorobenzene

-
-
2,4-dinitrofluorobenzene
-
no inhibition
2-Phosphoglycolate

-
-
2-Phosphoglycolate
-
competitive inhibition
2-Phosphoglycolate
-
strong, competitive. Inhibition results in a large decrease in the unfolding rate constant of the protein. 2-phosphoglycolate shows similar binding affinities in the transition states for the rate-limiting steps of the forward and backward reactions, implicating that both transition states resemble each other in the active site architecture
2-Phosphoglycolate
-
competitive inhibition
2-Phosphoglycolate
-
competitive inhibition
3-phosphoglycerate

-
-
3-phosphoglycerate
binding at the active site with the dimer-interface site showing strong electrostatic anchoring of the phosphate group involving the Arg98 and Lys112 residues of TIM, comparisons of binding structures at the interface, overview
4,4'-Dithiopyridine

-
-
5,5'-dithio-bis(2-nitrobenzoic acid)

derivatizes four of the five Cys per subunit of dimeric protein, resulting in inactivation and dissociation of the dimer to stable monomers
5,5'-dithio-bis(2-nitrobenzoic acid)
about 50% inhibition at about 0.05 mM
5,5'-dithiobis(2-nitrobenzoate)

-
-
5,5'-dithiobis(2-nitrobenzoate)
-
no inhibition
5,5'-dithiobis(2-nitrobenzoate)
-
-
5,5'-dithiobis(2-nitrobenzoate)
-
-
6,6'-bi-1,3-benzothiazole-2,2'-diamine

-
irreversible inactivation. Not inhibitory on human, yeast, chicken, Plasmodium falciparum, and Entamoeba histolytica enzyme
6,6'-bi-1,3-benzothiazole-2,2'-diamine
-
irreversible inactivation. Not inhibitory on human, yeast, chicken, Plasmodium falciparum, and Entamoeba histolytica enzyme
6,6'-bi-1,3-benzothiazole-2,2'-diamine
-
91% inactivation at 0.05 mM, irreversible. Not inhibitory on human, yeast, chicken, Plasmodium falciparum, and Entamoeba histolytica enzyme
6,6'-bi-1,3-benzothiazole-2,2'-diamine
-
-
Acetylphosphate

-
-
Acetylphosphate
-
competitive
arsenate

-
-
arsenate
-
competitive inhibition
arsenate
-
competitive inhibition
AsO2-

-
-
AsO43-

-
competitive
ATP

-
-
ATP
-
5 mM, 39% inhibition
bromohydroxyacetone phosphate

suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
bromohydroxyacetone phosphate
suicide inhibitor
cyclo(Gly-Pro-Phe-Val-Phe-PSI[CS-NH]Phe)

-
no inhibition
cyclo(Gly-Pro-Phe-Val-Phe-PSI[CS-NH]Phe)
-
no inhibition
cyclo(Gly-Pro-Phe-Val-Phe-PSI[CS-NH]Phe)
-
no inhibition
cyclo(Gly-Pro-Phe-Val-Phe-PSI[CS-NH]Phe)
-
-
cyclo[Trp-Phe-D-Pro-Phe-Phe-Lys(benzyloxycarbonyl)-]

-
no inhibition
cyclo[Trp-Phe-D-Pro-Phe-Phe-Lys(benzyloxycarbonyl)-]
-
no inhibition
cyclo[Trp-Phe-D-Pro-Phe-Phe-Lys(benzyloxycarbonyl)-]
-
no inhibition
cyclo[Trp-Phe-D-Pro-Phe-Phe-Lys(benzyloxycarbonyl)-]
-
-
D-alpha-glycerophosphate

-
competitive
D-alpha-glycerophosphate
-
-
D-alpha-glycerophosphate
-
competitive
D-alpha-glycerophosphate
-
competitive
DL-glycidol phosphate

suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
H2O2

0.5 mM, 120 min, 30% loss of activity in presence and absence of glutathione
H2O2
the inhibition of the enzyme is negligible when incubated with 0.1 mM H2O2, whereas a 10% inhibition is observed with 1 mM H2O2
iodoacetate

-
-
iodoacetate
-
no inhibition
methyl methanethiosulfonate

-
no inhibition
methyl methanethiosulfonate
derivatizes three of the five Cys per subunit of dimeric protein and induces 50% of inactivation. Inactivation does not affect secondary structure nor induce dimer dissociation. Cys modification decreases thermal stability of the enzyme
methyl methanethiosulfonate
-
the sensitivity of enzyme from Trypanosoma cruzi is about 40times higher than that of Trypanosoma brucei and 200times higher than that of Leishmania mexicana
methyl methanethiosulfonate
-
-
methyl methanethiosulfonate
about 50% inhibition at about 0.05 mM
methyl methanethiosulfonate
-
no inhibition
methyl methanethiosulfonate
-
-
methyl methanethiosulfonate
-
derivatizes Cys14 to a methyl sulfide
methyl methanethiosulfonate
-
the sensitivity of enzyme from Trypanosoma cruzi is about 40times higher than that of Trypanosoma brucei and 200times higher than that of Leishmania mexicana
methyl methanethiosulfonate
-
the sensitivity of enzyme from Trypanosoma cruzi is about 40times higher than that of Trypanosoma brucei and 200times higher than that of Leishmania mexicana
methylmethane thiosulfonate

-
0.6 mM, 2 h, complete loss of activity, dissociates the dimeric enzyme inducing formation of a compact monomeric state
methylmethane thiosulfonate
-
induces 75% enzyme inactivation within 15 min at 0.25 mM concentration
methylmethane thiosulfonate
-
modification at C15 in the dimer interface, inducing abolition of catalysis and structural changes. Susceptibility of Trypanosoma cruzi enzyme to modification of C15 is nearly 100fold higher than susceptibility of C15 of Trypanosoma brucei
methylmethane thiosulfonate
-
60% residual activity at 0.1 mM
methylmethane thiosulfonate
inactivation by sulfhydroxylation
methylmethane thiosulfonate
-
modification at C15 in the dimer interface, inducing abolition of catalysis and structural changes. Susceptibility of Trypanosoma cruzi enzyme to modification of C15 is nearly 100fold higher than susceptibility of C15 of Trypanosoma brucei
methylmethane thiosulfonate
-
almost complete inhibition at 0.1 mM
methylmethane thiosulfonate
inactivation by sulfhydroxylation
N-ethylmaleimide

0.5 mM, 60 min, more than 90% loss of activity. Addition of dithiothreitol does not restore activity. In the presence of 1 mM glutathione, the addition of 0.5 mM N-ethylmaleimide does not significantly affect activity
p-hydroxymercuribenzoate

-
-
p-hydroxymercuribenzoate
-
-
PCMB

-
-
phosphate

competitive, occupies the substrate binding pocket
phosphoenolpyruvate

-
competitive
phosphoenolpyruvate
competitive
phosphoenolpyruvate
-
competitive
Phosphoglycolate

-
Phosphoglycolate
-
2-phosphoglycolate
Phosphoglycolate
-
competitive
Phosphoglycolate
-
2-phosphoglycolate
Phosphoglycolate
-
and its corresponding hydroxamate; competitive
Phosphoglycolate
-
competitive
Phosphoglycolate
-
2-phosphoglycolate
Phosphoglycolohydroxamate

the reaction-intermediate analogue binds to the active site with two hydrogen-bonding interactions between PGH and the Glu167 side-chain oxygen atoms
Phosphoglycolohydroxamate
-
-
Phosphoglycolohydroxamate
-
-
S-Phenyl-p-toluenethiosulfonate

-
no inhibition
S-Phenyl-p-toluenethiosulfonate
-
-
S-Phenyl-p-toluenethiosulfonate
-
-
S-Phenyl-p-toluenethiosulfonate
-
no inhibition
S-Phenyl-p-toluenethiosulfonate
-
-
SO42-

-
CuSO4
additional information

-
in drug-resistant SGC7901 cells induced by vincristine sulfate, triosephosphate isomerase is downregulated.The sensitivity of TPI-SGC7901/VCR cells to adriamycin, vincristine, 5-fluorouracil and cis-dichlorodiamine platinum, as well as the accumulation and retention to adriamycin, are significantly increased when compared to their control cell lines
-
additional information
-
TPI phosphorylation by cyclin A/Cdk2 kinase leads to reduced TPI activity, prevented by treatment with olomoucine, a specific inhibitor of Cdk2
-
additional information
-
not inhibited by 1,2,6-thiadiazine, phenazine 5,9-dioxide, and 1,3,4-oxathiazole
-
additional information
-
recombinant expression of Tau in CHO-K1 cells leads to increased protection of TPI against oxidative damage, but also to decreased enzyme activity, with unaltered TPI expression levels
-
additional information
ANWKCNGTLE, the peptide that corresponds to loop 1 of triosephosphate isomerase, residues 9-18, shows only negligible inhibition of wild-type enzyme and mutant enzymes Y74G and Y74C, with a fall in the enzymatic activity by only about 20% at 1000fold molar excess of the peptide
-
additional information
-
ANWKCNGTLE, the peptide that corresponds to loop 1 of triosephosphate isomerase, residues 9-18, shows only negligible inhibition of wild-type enzyme and mutant enzymes Y74G and Y74C, with a fall in the enzymatic activity by only about 20% at 1000fold molar excess of the peptide
-
additional information
-
stronger inhibition by alpha-(1,3)-mannooligosaccharides than with triose, binding constants, overview
-
additional information
-
brevifolin carboxylate derivatives isolated from Geranium bellum selectively inactivate the enzyme partially, no inhibition by methyl tri-O-methylbrevifolin carboxylate
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00004 - 50
D-glyceraldehyde 3-phosphate
0.59 - 25
dihydroxyacetone 3-phosphate
0.0025 - 18
dihydroxyacetone phosphate
0.26 - 2.5
glycerone phosphate
additional information
additional information
-
-
-
0.00004
D-glyceraldehyde 3-phosphate

mutant V167P/W168E, pH 7.5, 25°C
0.084
D-glyceraldehyde 3-phosphate
-
22°C, pH 7.6
0.091
D-glyceraldehyde 3-phosphate
-
mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
0.14
D-glyceraldehyde 3-phosphate
-
mutant C14S/A73C, 25°C, pH 7.4
0.14
D-glyceraldehyde 3-phosphate
-
mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C
0.16
D-glyceraldehyde 3-phosphate
mutant P168A, 25°C, pH 7.6
0.18
D-glyceraldehyde 3-phosphate
-
mutant E168D, pH 7.4, 25°C
0.19
D-glyceraldehyde 3-phosphate
-
recombinant wild-type enzyme
0.2
D-glyceraldehyde 3-phosphate
-
active form P2 of TIM, at 25°C, pH 7.4
0.21
D-glyceraldehyde 3-phosphate
pH 8.0, 90°C
0.21
D-glyceraldehyde 3-phosphate
wild-type, pH 7.4, 25°C
0.22
D-glyceraldehyde 3-phosphate
-
plastidic triosephosphate isomerase
0.23
D-glyceraldehyde 3-phosphate
wild-type, pH 7.4, 25°C
0.23
D-glyceraldehyde 3-phosphate
wild-type, pH 7.4, 25°C
0.24
D-glyceraldehyde 3-phosphate
mutant enzyme S227A
0.245
D-glyceraldehyde 3-phosphate
pH 6.5, 70°C
0.25
D-glyceraldehyde 3-phosphate
wild-type enzyme
0.25
D-glyceraldehyde 3-phosphate
-
D-glyceraldehyde 3-phosphate
0.25
D-glyceraldehyde 3-phosphate
-
isoenzyme A
0.25
D-glyceraldehyde 3-phosphate
-
wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C
0.25
D-glyceraldehyde 3-phosphate
-
wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C
0.25
D-glyceraldehyde 3-phosphate
-
wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
0.26
D-glyceraldehyde 3-phosphate
wild-type, pH 7.6, 25°C
0.26
D-glyceraldehyde 3-phosphate
wild-type, 25°C, pH 7.6
0.27
D-glyceraldehyde 3-phosphate
-
mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
0.28
D-glyceraldehyde 3-phosphate
mutant enzyme S227N
0.28
D-glyceraldehyde 3-phosphate
-
in complex with mutant C15A of Trypanosoma cruzi enzyme, pH 7.4, 25°C
0.28
D-glyceraldehyde 3-phosphate
-
mutant C15A in complex with intact monomer from Trypanosoma brucei enzyme, pH 7.4, 25°C
0.28
D-glyceraldehyde 3-phosphate
mutant W162F
0.29
D-glyceraldehyde 3-phosphate
-
cytoplasmic triosephosphate isomerase
0.29
D-glyceraldehyde 3-phosphate
wild-type, pH 7.5, 25°C
0.29
D-glyceraldehyde 3-phosphate
-
wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
0.3
D-glyceraldehyde 3-phosphate
-
recombinant enzyme
0.3
D-glyceraldehyde 3-phosphate
pH 8.0, 25°C
0.3
D-glyceraldehyde 3-phosphate
-
25°C, pH 7.4, mutant enzyme C126S
0.3
D-glyceraldehyde 3-phosphate
-
active form P1 of TIM, at 25°C, pH 7.4
0.3
D-glyceraldehyde 3-phosphate
mutant I45V, pH 7.4, 25°C
0.303
D-glyceraldehyde 3-phosphate
-
mutant I170T, pH not specified in the publication, temperature not specified in the publication
0.31
D-glyceraldehyde 3-phosphate
-
-
0.31
D-glyceraldehyde 3-phosphate
-
in complex with monomer from Trypanosoma brucei, pH 7.4, 25°C
0.31
D-glyceraldehyde 3-phosphate
-
in complex with monomer from Trypanosoma cruzi, pH 7.4, 25°C
0.31
D-glyceraldehyde 3-phosphate
mutant enzyme C13D, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication
0.32
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14T
0.32
D-glyceraldehyde 3-phosphate
mutant I45L, pH 7.4, 25°C
0.328
D-glyceraldehyde 3-phosphate
-
in 50 mM Tris, pH 7.6, at 25°C
0.33
D-glyceraldehyde 3-phosphate
-
isoenzyme B
0.33
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14P
0.33
D-glyceraldehyde 3-phosphate
pH 7.6, mutant enzyme Y74C
0.34
D-glyceraldehyde 3-phosphate
-
enzyme form I
0.34
D-glyceraldehyde 3-phosphate
mutant enzyme R191S
0.34
D-glyceraldehyde 3-phosphate
pH 7.6, mutant enzyme Y74G
0.35
D-glyceraldehyde 3-phosphate
-
0.35
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, wild-type enzyme
0.35
D-glyceraldehyde 3-phosphate
pH 7.6, wild-type enzyme
0.35
D-glyceraldehyde 3-phosphate
-
mutant C14S/S71C, 25°C, pH 7.4
0.35
D-glyceraldehyde 3-phosphate
wild-type, 25°C, pH not specified in the publication
0.35
D-glyceraldehyde 3-phosphate
wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
0.35
D-glyceraldehyde 3-phosphate
wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication
0.35
D-glyceraldehyde 3-phosphate
wild type enzyme, in TEA buffer (pH 7.6, 100 mM), at 25°C
0.38
D-glyceraldehyde 3-phosphate
-
wild-type, 25°C, pH 7.4
0.39
D-glyceraldehyde 3-phosphate
pH 7.6, wild-type enzyme
0.39
D-glyceraldehyde 3-phosphate
mutant I45A, pH 7.4, 25°C
0.39
D-glyceraldehyde 3-phosphate
mutant lacking loop 3, pH 7.4, 25°C
0.4
D-glyceraldehyde 3-phosphate
-
-
0.4067
D-glyceraldehyde 3-phosphate
-
recombinant enzyme
0.42
D-glyceraldehyde 3-phosphate
-
-
0.42
D-glyceraldehyde 3-phosphate
-
-
0.42
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14V
0.42
D-glyceraldehyde 3-phosphate
-
mutant C15A, pH 7.4, 25°C
0.42
D-glyceraldehyde 3-phosphate
mutant enzyme C13E, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication
0.43
D-glyceraldehyde 3-phosphate
-
enzyme form II
0.43
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14A
0.43
D-glyceraldehyde 3-phosphate
mutant W196F
0.43
D-glyceraldehyde 3-phosphate
-
in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C
0.44
D-glyceraldehyde 3-phosphate
-
-
0.44
D-glyceraldehyde 3-phosphate
wild-type, 25°C, pH not specified in the publication
0.45
D-glyceraldehyde 3-phosphate
-
wild-type, pH 7.4, 25°C
0.45
D-glyceraldehyde 3-phosphate
wild-type, 25°C, pH not specified in the publication
0.45
D-glyceraldehyde 3-phosphate
-
in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C
0.46
D-glyceraldehyde 3-phosphate
-
wild-type, pH 7.4, 25°C
0.47
D-glyceraldehyde 3-phosphate
-
30°C, pH 7.6, wild-type enzyme
0.47
D-glyceraldehyde 3-phosphate
in 100 mM triethanolamine, pH 7.4, at 25°C
0.48
D-glyceraldehyde 3-phosphate
-
mutant C14S/S79C, 25°C, pH 7.4
0.48
D-glyceraldehyde 3-phosphate
mutant C127S, pH 7.8, 30°C
0.5
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14S
0.5
D-glyceraldehyde 3-phosphate
-
mutant C14S, 25°C, pH 7.4
0.5
D-glyceraldehyde 3-phosphate
mutant enzyme E165A, in TEA buffer (pH 7.6, 100 mM), at 25°C
0.5
D-glyceraldehyde 3-phosphate
mutant C127S/C218S, pH 7.8, 30°C
0.5
D-glyceraldehyde 3-phosphate
mutant lacking loop 3, pH 7.4, 25°C
0.51
D-glyceraldehyde 3-phosphate
mutant V45A, pH 7.4, 25°C
0.52
D-glyceraldehyde 3-phosphate
mutant I45F, pH 7.4, 25°C
0.53
D-glyceraldehyde 3-phosphate
-
25°C, pH 7.4, dimeric enzyme form
0.55
D-glyceraldehyde 3-phosphate
-
enzyme form III
0.6
D-glyceraldehyde 3-phosphate
-
cytosolic enzyme
0.6
D-glyceraldehyde 3-phosphate
-
D-glyceraldehyde 3-phosphate
0.6
D-glyceraldehyde 3-phosphate
mutant F12W
0.61
D-glyceraldehyde 3-phosphate
mutant A178L, pH 7.6, 25°C
0.62
D-glyceraldehyde 3-phosphate
-
in complex with mutant C15A of Trypanosoma cruzi enzyme, after modification by methylmethane thiosulfonate, pH 7.4, 25°C
0.62
D-glyceraldehyde 3-phosphate
-
mutant C15A in complex with intact monomer from Trypanosoma brucei enzyme, after modification by methylmethane thiosulfonate, pH 7.4, 25°C
0.62
D-glyceraldehyde 3-phosphate
wild-type, pH 7.6, 23°C
0.68
D-glyceraldehyde 3-phosphate
-
chloroplastic enzyme
0.68
D-glyceraldehyde 3-phosphate
pH 8.0, 90°C
0.687
D-glyceraldehyde 3-phosphate
-
mutant I170V, pH not specified in the publication, temperature not specified in the publication
0.69
D-glyceraldehyde 3-phosphate
-
mutant C15A in complex with mutant E168D of Trypanosoma brucei, pH 7.4, 25°C
0.69
D-glyceraldehyde 3-phosphate
-
mutant E168D in complex with mutant C15A of Trypanosoma cruzi, pH 7.4, 25°C
0.7
D-glyceraldehyde 3-phosphate
mutant Y208T/S211G, pH 7.5, 25°C
0.73
D-glyceraldehyde 3-phosphate
mutant W75F/W162F/W196F
0.74
D-glyceraldehyde 3-phosphate
mutant W75F
0.75
D-glyceraldehyde 3-phosphate
-
mutant C14S/A69C, 25°C, pH 7.4
0.78
D-glyceraldehyde 3-phosphate
wildtype
0.8
D-glyceraldehyde 3-phosphate
-
0.8
D-glyceraldehyde 3-phosphate
-
25°C, pH 7.4, mutant enzyme C126A
0.8
D-glyceraldehyde 3-phosphate
mutant T75S, pH 7.6, 23°C
0.84
D-glyceraldehyde 3-phosphate
pH 7.0, 25°C
0.86
D-glyceraldehyde 3-phosphate
mutant T75N, pH 7.6, 23°C
0.87
D-glyceraldehyde 3-phosphate
-
25°C, pH 7.4, tetrameric enzyme form
0.87
D-glyceraldehyde 3-phosphate
mutant F96A, 25°C, pH not specified in the publication
0.9
D-glyceraldehyde 3-phosphate
-
-
0.9
D-glyceraldehyde 3-phosphate
-
-
0.91
D-glyceraldehyde 3-phosphate
-
mutant D225Q, pH 7.4, 25°C
0.91
D-glyceraldehyde 3-phosphate
mutant T75V, pH 7.6, 23°C
0.94
D-glyceraldehyde 3-phosphate
mutant W173F
0.97
D-glyceraldehyde 3-phosphate
presence of methyl methanethiosulfonate
1
D-glyceraldehyde 3-phosphate
wild-type, pH 7.5, 25°C
1
D-glyceraldehyde 3-phosphate
mutant W162F/W173F/W196F
1
D-glyceraldehyde 3-phosphate
mutant enzyme C126V, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
1.02
D-glyceraldehyde 3-phosphate
mutant F96S/L167V, 25°C, pH not specified in the publication
1.03
D-glyceraldehyde 3-phosphate
-
pH 7.6, wild-type enzyme
1.09
D-glyceraldehyde 3-phosphate
mutant K17A/Y46A/D48F/Q82A/D85S , pH 7.4, 25°C
1.1
D-glyceraldehyde 3-phosphate
mutant enzyme R191A
1.1
D-glyceraldehyde 3-phosphate
-
25°C, pH 7., wild-type enzyme
1.1
D-glyceraldehyde 3-phosphate
-
wild type enzyme, in 30 mM TEA at pH 7.5, at 25°C
1.12
D-glyceraldehyde 3-phosphate
mutant W75F/W162F/W173F
1.13
D-glyceraldehyde 3-phosphate
-
wild-type, pH 7.4, 25°C
1.15
D-glyceraldehyde 3-phosphate
mutant T75C, pH 7.6, 23°C
1.2
D-glyceraldehyde 3-phosphate
pH 8.0, 25°C
1.2
D-glyceraldehyde 3-phosphate
-
30°C, pH 7.6, mutant enzyme V167G/W168G
1.2
D-glyceraldehyde 3-phosphate
mutant K17L/Y46F/D48F/Q82F/D85L, pH 7.4, 25°C
1.2
D-glyceraldehyde 3-phosphate
pH 7.6, mutant F96W
1.2
D-glyceraldehyde 3-phosphate
mutant enzyme C126T, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
1.2
D-glyceraldehyde 3-phosphate
mutant enzyme E97Q, in TEA buffer (pH 7.6, 100 mM), at 25°C
1.2
D-glyceraldehyde 3-phosphate
mutant F96S/S73A, 25°C, pH not specified in the publication
1.26
D-glyceraldehyde 3-phosphate
mutant K17L/Y46F/D48Y/Q82A/D85A, pH 7.4, 25°C
1.27
D-glyceraldehyde 3-phosphate
-
-
1.29
D-glyceraldehyde 3-phosphate
mutant I45Y, pH 7.4, 25°C
1.32
D-glyceraldehyde 3-phosphate
wild-type, pH 7.8, 30°C
1.373
D-glyceraldehyde 3-phosphate
-
wild-type, pH not specified in the publication, temperature not specified in the publication
1.4
D-glyceraldehyde 3-phosphate
wild-type, pH 7.4, 25°C
1.4
D-glyceraldehyde 3-phosphate
mutant enzyme C126S, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
1.5
D-glyceraldehyde 3-phosphate
-
at pH 7.5 and 37°C
1.5
D-glyceraldehyde 3-phosphate
mutant enzyme C126A, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
1.5
D-glyceraldehyde 3-phosphate
mutant enzyme C126M, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
1.67
D-glyceraldehyde 3-phosphate
pH 8.0, 25°C
1.68
D-glyceraldehyde 3-phosphate
mutant C218S, pH 7.8, 30°C
1.78
D-glyceraldehyde 3-phosphate
mutant I45G, pH 7.4, 25°C
1.8
D-glyceraldehyde 3-phosphate
mutant S211G, pH 7.5, 25°C
1.85
D-glyceraldehyde 3-phosphate
mutant F96Y, 25°C, pH not specified in the publication
1.9
D-glyceraldehyde 3-phosphate
-
wild-type enzyme
1.9
D-glyceraldehyde 3-phosphate
-
pH 7.6, wild-type enzyme
1.9
D-glyceraldehyde 3-phosphate
mutant W75F/W173F/W196F
2.18
D-glyceraldehyde 3-phosphate
pH 7.6, mutant F96S
2.2
D-glyceraldehyde 3-phosphate
mutant F96S, 25°C, pH not specified in the publication
2.4
D-glyceraldehyde 3-phosphate
mutant Y208F, pH 7.5, 25°C
2.44
D-glyceraldehyde 3-phosphate
mutant K17P/Y46A/D48L/Q82T/D85A, pH 7.4, 25°C
2.49
D-glyceraldehyde 3-phosphate
mutant D213A, pH 7.5, 25°C
2.49
D-glyceraldehyde 3-phosphate
-
mutant D213A, pH 7.5, 25°C
2.62
D-glyceraldehyde 3-phosphate
pH 7.6, mutant F96H
2.9
D-glyceraldehyde 3-phosphate
mutant Y208A, pH 7.5, 25°C
3.04
D-glyceraldehyde 3-phosphate
in 100 mM triethanolamine (pH 7.4), at 25°C
3.27
D-glyceraldehyde 3-phosphate
mutant D213Q, pH 7.5, 25°C
3.27
D-glyceraldehyde 3-phosphate
-
mutant D213Q, pH 7.5, 25°C
3.3
D-glyceraldehyde 3-phosphate
monomeric mutant A178L, pH 7.6, 25°C
3.4
D-glyceraldehyde 3-phosphate
mutant Y208T, pH 7.5, 25°C
3.46
D-glyceraldehyde 3-phosphate
wild-type, pH 7.5, 25°C
3.46
D-glyceraldehyde 3-phosphate
-
wild-type, pH 7.5, 25°C
3.56
D-glyceraldehyde 3-phosphate
mutant K183S, pH 7.5, 25°C
3.56
D-glyceraldehyde 3-phosphate
-
mutant K183S, pH 7.5, 25°C
3.6
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14F
3.7
D-glyceraldehyde 3-phosphate
-
30°C, pH 7.6, mutant enzyme K174G/T175G/A176G
3.8
D-glyceraldehyde 3-phosphate
monomeric wild-type, pH 7.6, 25°C
3.9
D-glyceraldehyde 3-phosphate
mutant Y208S, pH 7.5, 25°C
4
D-glyceraldehyde 3-phosphate
-
30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G
4.8
D-glyceraldehyde 3-phosphate
-
mutant A238S
4.8
D-glyceraldehyde 3-phosphate
-
pH 7.6, mutant enzyme A238S
8.6
D-glyceraldehyde 3-phosphate
presence of 2-carboxyethyl methanethiosulfonate
9.09
D-glyceraldehyde 3-phosphate
mutant K183A, pH 7.5, 25°C
9.09
D-glyceraldehyde 3-phosphate
-
mutant K183A, pH 7.5, 25°C
12
D-glyceraldehyde 3-phosphate
mutant S211A, pH 7.5, 25°C
50
D-glyceraldehyde 3-phosphate
-
mutant enzyme K12G, in 30 mM TEA at pH 7.5, at 25°C
0.59
dihydroxyacetone 3-phosphate

wild-type, pH 7.5, 25°C
1.7
dihydroxyacetone 3-phosphate
wild-type, pH 7.5, 25°C
2.3
dihydroxyacetone 3-phosphate
-
at pH 7.5 and 37°C
3.2
dihydroxyacetone 3-phosphate
mutant S211G, pH 7.5, 25°C
4
dihydroxyacetone 3-phosphate
mutant Y208T/S211G, pH 7.5, 25°C
11
dihydroxyacetone 3-phosphate
mutant Y208T, pH 7.5, 25°C
17
dihydroxyacetone 3-phosphate
mutant Y208A, pH 7.5, 25°C
17
dihydroxyacetone 3-phosphate
mutant Y208F, pH 7.5, 25°C
25
dihydroxyacetone 3-phosphate
mutant Y208S, pH 7.5, 25°C
0.0025
dihydroxyacetone phosphate

in 100 mM Tris pH 7.8, at 25°C
0.077
dihydroxyacetone phosphate
-
mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C
0.49
dihydroxyacetone phosphate
-
mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
0.5
dihydroxyacetone phosphate
mutant P168A, 25°C, pH 7.6
0.59
dihydroxyacetone phosphate
-
wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
0.6
dihydroxyacetone phosphate
-
25°C, pH 7.4, mutant enzyme C126S
0.7
dihydroxyacetone phosphate
-
wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C
0.7
dihydroxyacetone phosphate
-
wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C
0.7
dihydroxyacetone phosphate
-
wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
0.9
dihydroxyacetone phosphate
wild-type, pH 7.6, 25°C
0.9
dihydroxyacetone phosphate
wild-type, 25°C, pH 7.6
0.97
dihydroxyacetone phosphate
-
30°C, pH 7.6, wild-type enzyme
1.1
dihydroxyacetone phosphate
-
25°C, pH 7.4, dimeric enzyme form
1.4
dihydroxyacetone phosphate
mutant A178L, pH 7.6, 25°C
2.1
dihydroxyacetone phosphate
-
25°C, pH 7.4, wild-type enzyme
4
dihydroxyacetone phosphate
-
mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
7.6
dihydroxyacetone phosphate
monomeric wild-type, pH 7.6, 25°C
9.3
dihydroxyacetone phosphate
monomeric mutant A178L, pH 7.6, 25°C
11
dihydroxyacetone phosphate
-
30°C, pH 7.6, mutant enzyme V167G/W168G
15
dihydroxyacetone phosphate
-
30°C, pH 7.6, mutant enzyme K174G/T175G/A176G
18
dihydroxyacetone phosphate
-
30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G
0.26
glycerone phosphate

-
isoenzyme B
0.37
glycerone phosphate
-
isoenzyme A
0.59
glycerone phosphate
-
-
0.59
glycerone phosphate
-
-
0.67
glycerone phosphate
mutant enzyme R191A
0.69
glycerone phosphate
-
enzyme form III
0.7
glycerone phosphate
mutant enzyme D227N
0.75
glycerone phosphate
-
-
0.812
glycerone phosphate
pH 6.5, 70°C
0.82
glycerone phosphate
-
enzyme form II
0.88
glycerone phosphate
-
-
0.9
glycerone phosphate
mutant enzyme D227A
1
glycerone phosphate
wild-type enzyme
1.1
glycerone phosphate
-
recombinant wild-type enzyme
1.1
glycerone phosphate
-
glycerone phosphate
1.2
glycerone phosphate
-
-
1.23
glycerone phosphate
-
-
1.3
glycerone phosphate
-
recombinant enzyme
1.5
glycerone phosphate
-
enzyme form I
1.5
glycerone phosphate
-
glycerone phosphate, cytosolic enzyme
1.6
glycerone phosphate
mutant enzyme R191S
2.5
glycerone phosphate
-
chloroplastic enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.013 - 57500000
D-glyceraldehyde 3-phosphate
4.5 - 860
dihydroxyacetone 3-phosphate
0.06 - 800
dihydroxyacetone phosphate
63 - 1080
glycerone phosphate
additional information
additional information
-
0.013
D-glyceraldehyde 3-phosphate

mutant T75N, pH 7.6, 23°C
0.03
D-glyceraldehyde 3-phosphate
mutant 170-173del, pH 7.5, 25°C
0.034
D-glyceraldehyde 3-phosphate
mutant T75C, pH 7.6, 23°C
0.038
D-glyceraldehyde 3-phosphate
mutant T75S, pH 7.6, 23°C
0.038
D-glyceraldehyde 3-phosphate
mutant T75V, pH 7.6, 23°C
0.04
D-glyceraldehyde 3-phosphate
wild-type, pH 7.6, 23°C
0.25
D-glyceraldehyde 3-phosphate
monomeric mutant A178L, pH 7.6, 25°C
0.31
D-glyceraldehyde 3-phosphate
mutant lacking loop 3, pH 7.4, 25°C
0.6
D-glyceraldehyde 3-phosphate
-
mutant enzyme K12G, in 30 mM TEA at pH 7.5, at 25°C
0.69
D-glyceraldehyde 3-phosphate
mutant K17L/Y46F/D48F/Q82F/D85L, pH 7.4, 25°C
1
D-glyceraldehyde 3-phosphate
mutant enzyme E97Q, in TEA buffer (pH 7.6, 100 mM), at 25°C
1.04
D-glyceraldehyde 3-phosphate
mutant K17L/Y46F/D48Y/Q82A/D85A, pH 7.4, 25°C
1.5
D-glyceraldehyde 3-phosphate
mutant lacking loop 3, pH 7.4, 25°C
1.7
D-glyceraldehyde 3-phosphate
-
30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G
1.9
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14F
2.2
D-glyceraldehyde 3-phosphate
monomeric wild-type, pH 7.6, 25°C
3.8
D-glyceraldehyde 3-phosphate
mutant F96A, 25°C, pH not specified in the publication
4
D-glyceraldehyde 3-phosphate
mutant F96S/L167V, 25°C, pH not specified in the publication
4.2
D-glyceraldehyde 3-phosphate
mutant enzyme E165A, in TEA buffer (pH 7.6, 100 mM), at 25°C
4.3
D-glyceraldehyde 3-phosphate
wild-type, 25°C, pH not specified in the publication
4.6
D-glyceraldehyde 3-phosphate
mutant F96S/S73A, 25°C, pH not specified in the publication
5.5
D-glyceraldehyde 3-phosphate
mutant F96Y, 25°C, pH not specified in the publication
6.2
D-glyceraldehyde 3-phosphate
mutant F96S, 25°C, pH not specified in the publication
11
D-glyceraldehyde 3-phosphate
-
mutant E168D, pH 7.4, 25°C
13
D-glyceraldehyde 3-phosphate
mutant Y208F, pH 7.5, 25°C
14.8
D-glyceraldehyde 3-phosphate
mutant C127S/C218S, pH 7.8, 30°C
15.7
D-glyceraldehyde 3-phosphate
mutant I45Y, pH 7.4, 25°C
16
D-glyceraldehyde 3-phosphate
-
mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
17.7
D-glyceraldehyde 3-phosphate
mutant I45F, pH 7.4, 25°C
20
D-glyceraldehyde 3-phosphate
mutant V167P/W168E, pH 7.5, 25°C
24
D-glyceraldehyde 3-phosphate
-
mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
60
D-glyceraldehyde 3-phosphate
mutant P168A, 25°C, pH 7.6
67
D-glyceraldehyde 3-phosphate
pH 7.6, mutant enzyme Y74G
70.4
D-glyceraldehyde 3-phosphate
mutant C127S, pH 7.8, 30°C
72.8
D-glyceraldehyde 3-phosphate
mutant I45G, pH 7.4, 25°C
76.7
D-glyceraldehyde 3-phosphate
mutant V45A, pH 7.4, 25°C
78.3
D-glyceraldehyde 3-phosphate
mutant I45A, pH 7.4, 25°C
160
D-glyceraldehyde 3-phosphate
mutant enzyme C126V, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
185
D-glyceraldehyde 3-phosphate
pH 7.6, mutant F96H
190
D-glyceraldehyde 3-phosphate
mutant enzyme C126M, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
220
D-glyceraldehyde 3-phosphate
-
mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C
240
D-glyceraldehyde 3-phosphate
-
30°C, pH 7.6, mutant enzyme V167G/W168G
261
D-glyceraldehyde 3-phosphate
-
30°C, pH 7.6, mutant enzyme K174G/T175G/A176G
330
D-glyceraldehyde 3-phosphate
mutant enzyme C126T, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
483
D-glyceraldehyde 3-phosphate
-
mutant C14S/A73C, 25°C, pH 7.4
520
D-glyceraldehyde 3-phosphate
mutant Y208T/S211G, pH 7.5, 25°C
600
D-glyceraldehyde 3-phosphate
mutant enzyme C13D, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication
620
D-glyceraldehyde 3-phosphate
pH 7.6, mutant F96S
740
D-glyceraldehyde 3-phosphate
mutant Y208A, pH 7.5, 25°C
750
D-glyceraldehyde 3-phosphate
-
mutant C15A in complex with mutant E168D of Trypanosoma brucei, pH 7.4, 25°C
750
D-glyceraldehyde 3-phosphate
-
mutant E168D in complex with mutant C15A of Trypanosoma cruzi, pH 7.4, 25°C
750
D-glyceraldehyde 3-phosphate
mutant enzyme C126S, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
770
D-glyceraldehyde 3-phosphate
mutant enzyme C126A, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
833
D-glyceraldehyde 3-phosphate
-
in complex with mutant C15A of Trypanosoma cruzi enzyme, after modification by methylmethane thiosulfonate, pH 7.4, 25°C
833
D-glyceraldehyde 3-phosphate
-
mutant C15A in complex with intact monomer from Trypanosoma brucei enzyme, after modification by methylmethane thiosulfonate, pH 7.4, 25°C
940
D-glyceraldehyde 3-phosphate
mutant Y208S, pH 7.5, 25°C
1100
D-glyceraldehyde 3-phosphate
-
25°C, pH 7.4, mutant enzyme C126S
1330
D-glyceraldehyde 3-phosphate
wild-type, pH 7.4, 25°C
1330
D-glyceraldehyde 3-phosphate
wild-type, pH 7.4, 25°C
1367
D-glyceraldehyde 3-phosphate
mutant enzyme C13E, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication
1373
D-glyceraldehyde 3-phosphate
wild-type, pH 7.4, 25°C
1470
D-glyceraldehyde 3-phosphate
wild-type, pH 7.5, 25°C
1570
D-glyceraldehyde 3-phosphate
mutant D213A, pH 7.5, 25°C
1600
D-glyceraldehyde 3-phosphate
mutant enzyme S227N
1650
D-glyceraldehyde 3-phosphate
mutant I45V, pH 7.4, 25°C
1666
D-glyceraldehyde 3-phosphate
presence of 2-carboxyethyl methanethiosulfonate
1830
D-glyceraldehyde 3-phosphate
mutant D213Q, pH 7.5, 25°C
1900
D-glyceraldehyde 3-phosphate
mutant I45L, pH 7.4, 25°C
2000
D-glyceraldehyde 3-phosphate
mutant K183S, pH 7.5, 25°C
2100
D-glyceraldehyde 3-phosphate
-
wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C
2100
D-glyceraldehyde 3-phosphate
-
wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C
2100
D-glyceraldehyde 3-phosphate
-
wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
2170
D-glyceraldehyde 3-phosphate
-
in complex with mutant C15A of Trypanosoma cruzi enzyme, pH 7.4, 25°C
2170
D-glyceraldehyde 3-phosphate
-
mutant C15A in complex with intact monomer from Trypanosoma brucei enzyme, pH 7.4, 25°C
2330
D-glyceraldehyde 3-phosphate
mutant K183A, pH 7.5, 25°C
2417
D-glyceraldehyde 3-phosphate
pH 7.6, mutant enzyme Y74C
2420
D-glyceraldehyde 3-phosphate
pH 7.6, mutant enzyme Y74C
2450
D-glyceraldehyde 3-phosphate
-
25°C, pH 7.4, tetrameric enzyme form
2466
D-glyceraldehyde 3-phosphate
pH 7.6, mutant F96W
2500
D-glyceraldehyde 3-phosphate
mutant enzymeR191A
2600
D-glyceraldehyde 3-phosphate
mutant enzyme S227A
2600
D-glyceraldehyde 3-phosphate
-
mutant C14S/A69C, 25°C, pH 7.4
2670
D-glyceraldehyde 3-phosphate
-
mutant C15A, pH 7.4, 25°C
2690
D-glyceraldehyde 3-phosphate
mutant C218S, pH 7.8, 30°C
2800
D-glyceraldehyde 3-phosphate
mutant S211A, pH 7.5, 25°C
2967
D-glyceraldehyde 3-phosphate
-
mutant C14S/S79C, 25°C, pH 7.4
3100
D-glyceraldehyde 3-phosphate
-
25°C, pH 7.4, mutant enzyme C126A
3166
D-glyceraldehyde 3-phosphate
mutant W162F/W173F/W196F
3166
D-glyceraldehyde 3-phosphate
presence of methyl methanethiosulfonate
3200
D-glyceraldehyde 3-phosphate
wild-type, pH 7.5, 25°C
3200
D-glyceraldehyde 3-phosphate
-
mutant C14S/S71C, 25°C, pH 7.4
3200
D-glyceraldehyde 3-phosphate
-
wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
3333
D-glyceraldehyde 3-phosphate
mutant W162F
3372
D-glyceraldehyde 3-phosphate
in 100 mM triethanolamine (pH 7.4), at 25°C
3406
D-glyceraldehyde 3-phosphate
wild-type, pH 7.8, 30°C
3570
D-glyceraldehyde 3-phosphate
wild-type, 25°C, pH 7.6
3666
D-glyceraldehyde 3-phosphate
mutant W75F/W162F/W173F
3667
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14P
3670
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14P
3700
D-glyceraldehyde 3-phosphate
mutant Y208T, pH 7.5, 25°C
3830
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14T
3833
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14T
4000
D-glyceraldehyde 3-phosphate
-
-
4000
D-glyceraldehyde 3-phosphate
mutant enzyme R191S
4000
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14V
4160
D-glyceraldehyde 3-phosphate
mutant K17P/Y46A/D48L/Q82T/D85A, pH 7.4, 25°C
4166
D-glyceraldehyde 3-phosphate
mutant W196F
4170
D-glyceraldehyde 3-phosphate
-
recombinant enzyme
4183
D-glyceraldehyde 3-phosphate
-
wild-type, 25°C, pH 7.4
4300
D-glyceraldehyde 3-phosphate
pH 7.0, 25°C
4300
D-glyceraldehyde 3-phosphate
-
30°C, pH 7.6, wild-type enzyme
4300
D-glyceraldehyde 3-phosphate
wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), at 23°C
4300
D-glyceraldehyde 3-phosphate
wild type enzyme, in TEA buffer (pH 7.6, 100 mM), at 25°C
4330
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, wild-type enzyme
4333
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, wild-type enzyme
4333
D-glyceraldehyde 3-phosphate
mutant W75F/W173F/W196F
4400
D-glyceraldehyde 3-phosphate
wild-type, pH 7.6, 25°C
4467
D-glyceraldehyde 3-phosphate
pH 7.6, wild-type enzyme
4467
D-glyceraldehyde 3-phosphate
wild type enzyme, in 100 mM triethanolamine-HCl (pH 7.6), temperature not specified in the publication
4470
D-glyceraldehyde 3-phosphate
pH 7.6, wild-type enzyme
4500
D-glyceraldehyde 3-phosphate
-
wild-type, pH 7.4, 25°C
4500
D-glyceraldehyde 3-phosphate
-
in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C
4600
D-glyceraldehyde 3-phosphate
-
mutant D225Q, pH 7.4, 25°C
4667
D-glyceraldehyde 3-phosphate
-
pH 7.6, mutant enzyme A238S
4670
D-glyceraldehyde 3-phosphate
-
mutant A238S
4670
D-glyceraldehyde 3-phosphate
-
pH 7.6, mutant enzyme A238S
4700
D-glyceraldehyde 3-phosphate
-
25°C, pH 7., wild-type enzyme
4833
D-glyceraldehyde 3-phosphate
-
25°C, pH 7.4, dimeric enzyme form
5000
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14S
5000
D-glyceraldehyde 3-phosphate
-
in complex with monomer from Trypanosoma brucei, pH 7.4, 25°C
5000
D-glyceraldehyde 3-phosphate
-
in complex with monomer from Trypanosoma cruzi, pH 7.4, 25°C
5133
D-glyceraldehyde 3-phosphate
-
mutant C14S, 25°C, pH 7.4
5167
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14A
5167
D-glyceraldehyde 3-phosphate
wild-type, 25°C, pH not specified in the publication
5167
D-glyceraldehyde 3-phosphate
-
in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C
5170
D-glyceraldehyde 3-phosphate
-
pH 7.4, 25°C, mutant enzyme C14A
5170
D-glyceraldehyde 3-phosphate
-
wild-type, pH 7.4, 25°C
5300
D-glyceraldehyde 3-phosphate
mutant A178L, pH 7.6, 25°C
5500
D-glyceraldehyde 3-phosphate
mutant F12W
6000
D-glyceraldehyde 3-phosphate
wild-type enzyme
6000
D-glyceraldehyde 3-phosphate
-
recombinant enzyme
6000
D-glyceraldehyde 3-phosphate
wild-type, 25°C, pH not specified in the publication
6166
D-glyceraldehyde 3-phosphate
mutant W173F
6170
D-glyceraldehyde 3-phosphate
-
-
6400
D-glyceraldehyde 3-phosphate
wild-type, pH 7.4, 25°C
6900
D-glyceraldehyde 3-phosphate
-
wild-type, pH 7.4, 25°C
7000
D-glyceraldehyde 3-phosphate
-
wild type enzyme
7000
D-glyceraldehyde 3-phosphate
-
pH 7.6, wild-type enzyme
7020
D-glyceraldehyde 3-phosphate
mutant K17A/Y46A/D48F/Q82A/D85S , pH 7.4, 25°C
7166
D-glyceraldehyde 3-phosphate
mutant W75F/W162F/W196F
7300
D-glyceraldehyde 3-phosphate
-
wild type enzyme, in 30 mM TEA at pH 7.5, at 25°C
7500
D-glyceraldehyde 3-phosphate
mutant S211G, pH 7.5, 25°C
7666
D-glyceraldehyde 3-phosphate
-
7666
D-glyceraldehyde 3-phosphate
wildtype
8000
D-glyceraldehyde 3-phosphate
mutant W75F
8670
D-glyceraldehyde 3-phosphate
-
-
8700
D-glyceraldehyde 3-phosphate
-
at pH 7.5 and 37°C
8900
D-glyceraldehyde 3-phosphate
wild-type, pH 7.5, 25°C
9000
D-glyceraldehyde 3-phosphate
-
pH 7.6, wild-type enzyme
14670
D-glyceraldehyde 3-phosphate
wild-type, pH 7.5, 25°C
15670
D-glyceraldehyde 3-phosphate
mutant D213A, pH 7.5, 25°C
16700
D-glyceraldehyde 3-phosphate
-
-
18330
D-glyceraldehyde 3-phosphate
mutant D213Q, pH 7.5, 25°C
20000
D-glyceraldehyde 3-phosphate
mutant K183S, pH 7.5, 25°C
23330
D-glyceraldehyde 3-phosphate
mutant K183A, pH 7.5, 25°C
42330
D-glyceraldehyde 3-phosphate
pH 7.6, wild-type enzyme
68330
D-glyceraldehyde 3-phosphate
-
22°C, pH 7.6
1430000
D-glyceraldehyde 3-phosphate
pH 8.0, 25°C
1548000
D-glyceraldehyde 3-phosphate
pH 8.0, 25°C
25000000
D-glyceraldehyde 3-phosphate
pH 8.0, 90°C
57500000
D-glyceraldehyde 3-phosphate
pH 8.0, 90°C
4.5
dihydroxyacetone 3-phosphate

mutant Y208F, pH 7.5, 25°C
135
dihydroxyacetone 3-phosphate
mutant Y208T/S211G, pH 7.5, 25°C
210
dihydroxyacetone 3-phosphate
mutant Y208A, pH 7.5, 25°C
250
dihydroxyacetone 3-phosphate
mutant Y208S, pH 7.5, 25°C
327
dihydroxyacetone 3-phosphate
-
at pH 7.5 and 37°C
340
dihydroxyacetone 3-phosphate
wild-type, pH 7.5, 25°C
580
dihydroxyacetone 3-phosphate
mutant Y208T, pH 7.5, 25°C
810
dihydroxyacetone 3-phosphate
mutant S211G, pH 7.5, 25°C
860
dihydroxyacetone 3-phosphate
wild-type, pH 7.5, 25°C
0.06
dihydroxyacetone phosphate

monomeric mutant A178L, pH 7.6, 25°C
0.25
dihydroxyacetone phosphate
-
30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G
0.8
dihydroxyacetone phosphate
monomeric wild-type, pH 7.6, 25°C
4.7
dihydroxyacetone phosphate
-
mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C
6.5
dihydroxyacetone phosphate
-
mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
8
dihydroxyacetone phosphate
-
mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
19
dihydroxyacetone phosphate
mutant P168A, 25°C, pH 7.6
50
dihydroxyacetone phosphate
-
30°C, pH 7.6, mutant enzyme V167G/W168G
54
dihydroxyacetone phosphate
-
30°C, pH 7.6, mutant enzyme K174G/T175G/A176G
60
dihydroxyacetone phosphate
-
25°C, pH 7.4, mutant enzyme C126S
267
dihydroxyacetone phosphate
-
25°C, pH 7.4, dimeric enzyme form
300
dihydroxyacetone phosphate
-
wild type enzyme, in 100 mM TEA (pH 7.5), at 25°C
300
dihydroxyacetone phosphate
-
wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C
300
dihydroxyacetone phosphate
-
wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
340
dihydroxyacetone phosphate
-
wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C
430
dihydroxyacetone phosphate
-
30°C, pH 7.6, wild-type enzyme
500
dihydroxyacetone phosphate
-
25°C, pH 7.4, wild-type enzyme
500
dihydroxyacetone phosphate
mutant A178L, pH 7.6, 25°C
645
dihydroxyacetone phosphate
wild-type, 25°C, pH 7.6
730
dihydroxyacetone phosphate
in 100 mM Tris pH 7.8, at 25°C
800
dihydroxyacetone phosphate
wild-type, pH 7.6, 25°C
63
glycerone phosphate
