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Literature summary for 4.6.1.23 extracted from

  • Endo, Y.; Huber, P.W.; Wool, I.G.
    The ribonuclease activity of the cytotoxin alpha-sarcin. The characteristics of the enzymatic activity of alpha-sarcin with ribosomes and ribonucleic acids as substrates (1983), J. Biol. Chem., 258, 2662-2667.
    View publication on PubMed
Search for more literature for 4.6.1.23

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ inhibits hydrolysis of RNA Aspergillus giganteus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates hydrolysis of DNA Aspergillus giganteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Aspergillus giganteus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus giganteus
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 filamentous fungus, mold
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information with rat liver ribosomes or 60S ribosomal subunits as substrates alpha-sarcin cleaves a single oligonucleotide of about 488 residues, the alpha-fragment from the 3' end of 28 S rRNA, 40 S ribosomal subunits are not affected by the enzyme, hydrolysis of DNA requires large amounts of the toxin Aspergillus giganteus ?
-
 ?
RNA + H2O with total rRNA as substrate alpha-sarcin causes extensive progressive digestion of nucleic acids Aspergillus giganteus ?
-
 ?
rRNA + H2O
-
 Aspergillus giganteus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.917
-
 28 S rRNA pH 7.5, 37°C Aspergillus giganteus