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Literature summary for 4.4.1.14 extracted from

  • White, M.F.; Vasquez, J.; Yang, S.F.; Kirsch, J.F.
    Expression of apple 1-aminocyclopropane-1-carboxylate synthase in Escherichia coli: kinetic characterization of wild-type and active-site mutant forms (1994), Proc. Natl. Acad. Sci. USA, 91, 12428-12432.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Malus sylvestris

Protein Variants

Protein Variants Comment Organism
R407K increase in Km for S-adenosyl-L-methionine and drop in kcat/Km Malus sylvestris
Y233F 24-fold increase in the Km for S-adenosyl-L-methionine and no change in kcat Malus sylvestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 S-adenosyl-L-methionine
-
 Malus sylvestris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 crosslinking, gel filtration Malus sylvestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine Malus sylvestris biosynthesis of ethylene: plant hormone 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Organism

Organism UniProt Comment Textmining
Malus sylvestris
-
 apple
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine biosynthesis of ethylene: plant hormone Malus sylvestris 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Malus sylvestris