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Literature summary for 4.4.1.11 extracted from

  • Fukamachi, H.; Nakano, Y.; Okano, S.; Shibata, Y.; Abiko, Y.; Yamashita, Y.
    High production of methyl mercaptan by L-methionine-alpha-deamino-gamma-mercaptomethane lyase from Treponema denticola (2005), Biochem. Biophys. Res. Commun., 331, 127-131.
    View publication on PubMed
Search for more literature for 4.4.1.11

Application

Application Comment Organism
drug development methyl mercaptan may not only cause oral malodour but also play an important role in the pathogenicity of Treptonema denticola. The enzyme may be an exploitable target for novel chemotherapeutic drugs. An inhibitor of METase may represent a new class of compounds with the potential for preventing and treating oral malodor Treponema denticola

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Treponema denticola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
 L-methionine
-
 Treponema denticola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43500
-
 SDS-PAGE Treponema denticola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine + H2O Porphyromonas gingivalis
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O Treponema denticola
-
 methanethiol + NH3 + 2-oxobutanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-
Treponema denticola
-
 ATCC 35405
-

Purification (Commentary)

Purification (Comment) Organism
-
 Treponema denticola

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.6
-
-
 Treponema denticola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homocysteine + H2O
-
 Treponema denticola hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O
-
 Porphyromonas gingivalis methanethiol + NH3 + 2-oxobutanoate
-
 ?
L-methionine + H2O
-
 Treponema denticola methanethiol + NH3 + 2-oxobutanoate
-
 ?

Synonyms

Synonyms Comment Organism
L-methionine-alpha-deamino-gamma-mercaptomethane lyase
-
 Porphyromonas gingivalis
L-methionine-alpha-deamino-gamma-mercaptomethane lyase
-
 Treponema denticola
METase
-
 Porphyromonas gingivalis
METase
-
 Treponema denticola