Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis | Ixeridium dentatum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the N-terminal 44 amino acid residues leads to loss of 90% of catalytic activity | Ixeridium dentatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.5-8.0, 25°C | Ixeridium dentatum | |
14.9 | - |
2-trans,6-trans-farnesyl diphosphate | pH 7.5-8.0, 37°C | Ixeridium dentatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67100 | - |
x * 67100, DNA sequence calculation | Ixeridium dentatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | Ixeridium dentatum | - |
(+)-(10R)-germacrene A + diphosphate | - |
? | |
additional information | Ixeridium dentatum | (+)-germacrene A synthesis is the committed step in the biosynthesis of bitter sesquiterpene lactones in chicory | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ixeridium dentatum | - |
formerly Ixeris albiflora | - |
Purification (Comment) | Organism |
---|---|
partially, recombinant enzyme | Ixeridium dentatum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2E,6E)-farnesyl diphosphate = (+)-(R)-gemacrene A + diphosphate | the catalytic site is located at the N-terminal 44 amino acid residues | Ixeridium dentatum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | expression levels decline near the onset of flowering | Ixeridium dentatum | - |
root | - |
Ixeridium dentatum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | - |
Ixeridium dentatum | (+)-(10R)-germacrene A + diphosphate | - |
? | |
2-trans,6-trans-farnesyl diphosphate | - |
Ixeridium dentatum | (+)-(10R)-germacrene A + diphosphate | reaction product analysis by GC-MS and NMR | ? | |
additional information | (+)-germacrene A synthesis is the committed step in the biosynthesis of bitter sesquiterpene lactones in chicory | Ixeridium dentatum | ? | - |
? | |
additional information | no activity with geranyl diphosphate | Ixeridium dentatum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67100, DNA sequence calculation | Ixeridium dentatum |
Synonyms | Comment | Organism |
---|---|---|
(+)-germacrene A synthase | - |
Ixeridium dentatum |
GAS | - |
Ixeridium dentatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Ixeridium dentatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | recombinant enzyme | Ixeridium dentatum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ixeridium dentatum | DNA sequence calculation | - |
5.2 |