BRENDA - Enzyme Database
show all sequences of 4.2.3.108

Characterization of a root-specific Arabidopsis terpene synthase responsible for the formation of the volatile monoterpene 1,8-cineole

Chen, F.; Ro, D.K.; Petri, J.; Gershenzon, J.; Bohlmann, J.; Pichersky, E.; Tholl, D.; Plant Physiol. 135, 1956-1966 (2004) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0002
-
geranyl diphosphate
temperature not specified in the publication, pH not specified in the publication
Arabidopsis thaliana
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
or Mn2+, absolutely required. Fully active at 40 mM
Arabidopsis thaliana
Mn2+
or Mg2+, absolutely required. Fully active at 1 mM
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE
Arabidopsis thaliana
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
P0DI76
-
-
Arabidopsis thaliana
P0DI77
isoform 2
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
epidermis
promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone
Arabidopsis thaliana
-
root
promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone
Arabidopsis thaliana
-
stele
promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
geranyl diphosphate + H2O
-
718231
Arabidopsis thaliana
1,8-cineole + diphosphate
52% 1,8-cineole plus 0.6% (+)-alpha-thujene, 1.9% (2)-(1S)-alpha-pinene, 14.5% (-)-sabinene, 7.8% (2)-(1S)-beta-pinene, 13.3% myrcene, 4.0% (2)-(4S)-limonene, (E)-2.7% beta-ocimene, 0.8% terpinolene, and 2.4% alpha-terpineol
-
-
?
additional information
no substrate: farnesyl diphosphate
718231
Arabidopsis thaliana
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.001
-
geranyl diphosphate
temperature not specified in the publication, pH not specified in the publication
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0002
-
geranyl diphosphate
temperature not specified in the publication, pH not specified in the publication
Arabidopsis thaliana
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
or Mn2+, absolutely required. Fully active at 40 mM
Arabidopsis thaliana
Mn2+
or Mg2+, absolutely required. Fully active at 1 mM
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
epidermis
promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone
Arabidopsis thaliana
-
root
promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone
Arabidopsis thaliana
-
stele
promoter activity is primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
geranyl diphosphate + H2O
-
718231
Arabidopsis thaliana
1,8-cineole + diphosphate
52% 1,8-cineole plus 0.6% (+)-alpha-thujene, 1.9% (2)-(1S)-alpha-pinene, 14.5% (-)-sabinene, 7.8% (2)-(1S)-beta-pinene, 13.3% myrcene, 4.0% (2)-(4S)-limonene, (E)-2.7% beta-ocimene, 0.8% terpinolene, and 2.4% alpha-terpineol
-
-
?
additional information
no substrate: farnesyl diphosphate
718231
Arabidopsis thaliana
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.001
-
geranyl diphosphate
temperature not specified in the publication, pH not specified in the publication
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Arabidopsis thaliana
Other publictions for EC 4.2.3.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747873
Ruan
Isolation and characterizatio ...
Artemisia annua
Front. Plant Sci.
7
638
2016
-
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1
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1
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2
2
3
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1
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3
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1
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5
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1
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1
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1
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5
-
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2
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1
1
-
1
1
-
-
-
1
3
3
1
1
1
748984
Piechulla
The alpha-terpineol to 1,8-ci ...
Nicotiana suaveolens, Nicotiana forgetiana
Plant Physiol.
172
2120-2131
2016
-
-
2
-
12
-
-
4
1
2
-
2
-
2
-
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2
2
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2
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6
-
2
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4
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2
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12
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4
1
2
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2
-
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2
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2
-
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6
-
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-
-
4
-
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5
5
-
4
4
748974
Yahyaa
Identification, functional ch ...
Laurus nobilis
Plant Physiol.
169
1683-1697
2015
-
-
1
-
-
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-
-
1
1
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1
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1
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5
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1
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5
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2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
730626
Faehnrich
Characteristic alatoid cineole ...
Nicotiana noctiflora
Plant Mol. Biol.
85
135-145
2014
-
-
1
-
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-
-
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1
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2
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718036
Grausgruber-Groeger
Seasonal influence on gene exp ...
Salvia officinalis
J. Plant Physiol.
169
353-359
2012
-
-
-
-
-
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3
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1
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2
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1
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1
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-
1
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-
718223
Demissie
Cloning, functional characteri ...
Lavandula x intermedia
Plant Mol. Biol.
79
393-411
2012
-
-
1
-
-
-
-
1
-
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2
-
-
6
-
1
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2
1
1
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1
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1
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2
1
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-
-
1
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1
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1
-
-
730622
Faehnrich
Synthesis of cineole cassette ...
Nicotiana bonariensis, Nicotiana forgetiana, Nicotiana longiflora
Plant Mol. Biol.
79
537-553
2012
-
-
3
3
-
-
-
-
3
-
-
-
-
3
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6
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3
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3
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3
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6
-
-
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-
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-
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-
718141
Faehnrich
Product variability of the cin ...
Nicotiana langsdorffii, Nicotiana alata
Mol. Plant
4
965-984
2011
2
-
2
-
-
-
-
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2
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4
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1
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2
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4
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2
-
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-
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729591
Nakano
Identification of the first ba ...
Streptomyces clavuligerus, Streptomyces clavuligerus DSM 738
ChemBioChem
12
1988-1991
2011
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1
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1
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1
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2
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4
1
2
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1
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1
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1
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1
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4
1
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1
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-
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1
1
716036
Schmiderer
Influence of gibberellin and d ...
Salvia officinalis
J. Plant Physiol.
167
779-786
2010
-
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2
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1
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1
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-
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2
-
-
2
-
-
718201
Keszei
Functional and evolutionary re ...
Eucalyptus sideroxylon
Phytochemistry
71
844-852
2010
-
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1
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6
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718210
Kampranis
Rational conversion of substra ...
Salvia fruticosa
Plant Cell
19
1994-2005
2007
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1
10
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10
-
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-
6
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1
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1
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10
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1
10
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10
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1
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10
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10
10
718219
Roeder
Regulation of simultaneous syn ...
Nicotiana suaveolens
Plant Mol. Biol.
65
107-124
2007
-
-
1
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-
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-
-
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2
1
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9
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3
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1
1
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1
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1
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1
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3
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1
1
1
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1
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1
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1
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-
718231
Chen
Characterization of a root-spe ...
Arabidopsis thaliana
Plant Physiol.
135
1956-1966
2004
-
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1
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1
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2
1
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2
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1
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1
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717153
Peters
Alternative termination chemis ...
Salvia officinalis
Arch. Biochem. Biophys.
417
203-211
2003
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1
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1
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717701
Wise
Syn stereochemistry of cyclic ...
Salvia officinalis
J. Am. Chem. Soc.
124
8546-8547
2002
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3779
Wise
Monoterpene synthases from com ...
Salvia officinalis
J. Biol. Chem.
273
14891-14899
1998
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1
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1
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2
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1
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717147
Croteau
Biosynthesis of monoterpenes: ...
Salvia officinalis
Arch. Biochem. Biophys.
309
184-192
1994
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5
4
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2
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1
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5
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