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Literature summary for 4.2.3.108 extracted from
- Biosynthesis of monoterpenes: partial purification, characterization, and mechanism of action of 1,8-cineole synthase (1994), Arch. Biochem. Biophys., 309, 184-192.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Fluorogeranyl diphosphate | competitive | Salvia officinalis |  |
| 2-fluorolinalyl diphosphate | competitive | Salvia officinalis | |
| 4-hydroxymercuribenzoate | inhibition is reversed by presence of cysteine | Salvia officinalis | |
| benzyl bromide | - |
Salvia officinalis | |
| diethyl dicarbonate | inhibition is reversed by presence of hydroxylamine | Salvia officinalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
geranyl diphosphate | pH 6.3, 30°C | Salvia officinalis | |
| 0.002 | - |
(3R)-linalyl diphosphate | pH 6.3, 30°C | Salvia officinalis | |
| 0.0042 | - |
(3S)-linalyl diphosphate | pH 6.3, 30°C | Salvia officinalis | |
| 0.0045 | - |
neryl diphosphate | pH 6.3, 30°C | Salvia officinalis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | or Mn2+, absolutely required. Km value 5 mM | Salvia officinalis | |
| Mn2+ | or Mg2+, absolutely required. Km value 0.3 mM | Salvia officinalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration | Salvia officinalis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Glycerol | 10%, improves stability during the assay | Salvia officinalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salvia officinalis | O81191 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Salvia officinalis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| geranyl diphosphate + H2O = 1,8-cineole + diphosphate | substrate in left-hynded screw-sense orientation isomerizes to (3R)-linalyl diphosphate which in anti,endo-conformation cyclizes to the (4R)-alpha-terpinyl intermediate and thence to cineole following the addition of water | Salvia officinalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| secretory cell | - |
Salvia officinalis | - |
| trichome | glandular trichome | Salvia officinalis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3R)-linalyl diphosphate + H2O | - |
Salvia officinalis | ? + diphosphate | - |
? | |
| (3S)-linalyl diphosphate + H2O | - |
Salvia officinalis | ? + diphosphate | - |
? | |
| geranyl diphosphate + H2O | - |
Salvia officinalis | 1,8-cineole + diphosphate | water is the sole source of the ether oxygen atom of 1,8-cineole | ? | |
| neryl diphosphate + H2O | - |
Salvia officinalis | ? + diphosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Salvia officinalis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0032 | - |
2-Fluorogeranyl diphosphate | pH 6.3, 30°C | Salvia officinalis | |
| 0.0048 | - |
2-fluorolinalyl diphosphate | pH 6.3, 30°C | Salvia officinalis | |
| 0.005 | - |
4-hydroxymercuribenzoate | pH 6.3, 30°C | Salvia officinalis | |
| 0.3 | - |
diethyl dicarbonate | pH 6.3, 30°C | Salvia officinalis | |
| 0.85 | - |
benzyl bromide | pH 6.3, 30°C | Salvia officinalis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Salvia officinalis | isoelectric focusing | - |
4.6 |
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