General Stability | Organism |
---|---|
stable to freeze-drying, retains activity for several months in this form | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 0.1 M: activation, higher concentrations cause lower activity or precipitation of alginate | Klebsiella aerogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Klebsiella aerogenes | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 0.1-0.3 M, activation | Klebsiella aerogenes | |
Mg2+ | 0.05-0.1 M: activation, higher concentrations cause lower activity or precipitation of alginate | Klebsiella aerogenes | |
Na+ | 0.1-0.3 M, activation | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly-alpha-L-guluronic acid | Klebsiella aerogenes | - |
unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | with a smaller proportion of the homologous tetrasaccharide | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
type 25, intracellular and extracellular enzymes | - |
Purification (Comment) | Organism |
---|---|
partial, ammonium sulfate precipitation and ion-exchange chromatography | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-guluronosyl linkage in alginate | - |
Klebsiella aerogenes | ? | - |
? | |
additional information | requires high salt concentrations for maximal activity, no action on: laminarin, dextran, heparin, chondroitin sulfate, pullulan, yeast mannan, lichenin or porphyran, slight amylase activity with amylose and glycogen, beta-elimination mechanism, three-step reaction | Klebsiella aerogenes | ? | - |
? | |
poly-alpha-L-guluronic acid | - |
Klebsiella aerogenes | ? | - |
? | |
poly-alpha-L-guluronic acid | - |
Klebsiella aerogenes | unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | with a smaller proportion of the homologous tetrasaccharide | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Klebsiella aerogenes |