Cloned (Comment) | Organism |
---|---|
into the pGEM-T easy vector for sequencing | Achromobacter xylosoxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
determined by SDS-PAGE | Achromobacter xylosoxidans |
63410 | - |
calculated | Achromobacter xylosoxidans |
270000 | - |
native enzyme, tetramer | Achromobacter xylosoxidans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate | Achromobacter xylosoxidans | substrate specificity 100% | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter xylosoxidans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
after ammonium sulfate precipitation chromatography on a Phenyl-Sepharose, a Q-Sepharose, a Mono Q, and a HiTrap desalting column is performed | Achromobacter xylosoxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
nitrogen source urea | Achromobacter xylosoxidans |
0.011 | - |
carbon source DL-glutamate | Achromobacter xylosoxidans |
0.013 | - |
carbon source DL-alanine | Achromobacter xylosoxidans |
0.019 | - |
nitrogen source bacto peptone | Achromobacter xylosoxidans |
0.025 | - |
carbon source D-glucose | Achromobacter xylosoxidans |
0.027 | - |
carbon source D-galactose | Achromobacter xylosoxidans |
0.027 | - |
nitrogen source yeast extract | Achromobacter xylosoxidans |
0.031 | - |
nitrogen source (NH4)SO4 | Achromobacter xylosoxidans |
0.031 | - |
nitrogen source glucosamine | Achromobacter xylosoxidans |
0.036 | - |
nitrogen source H4NO3 | Achromobacter xylosoxidans |
0.038 | - |
carbon source D-galactonate | Achromobacter xylosoxidans |
0.04 | - |
nitrogen source casamino acid | Achromobacter xylosoxidans |
0.042 | - |
carbon source maltose | Achromobacter xylosoxidans |
0.046 | - |
carbon source D-arabinose | Achromobacter xylosoxidans |
0.05 | - |
nitrogen source NH4Cl | Achromobacter xylosoxidans |
0.057 | - |
carbon source sucrose | Achromobacter xylosoxidans |
0.111 | - |
nitrogen source malt extract | Achromobacter xylosoxidans |
0.13 | - |
carbon source D-gluconate | Achromobacter xylosoxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabonate | substrate specificity 4% | Achromobacter xylosoxidans | ? | - |
? | |
D-fuconate | substrate specificity 18% | Achromobacter xylosoxidans | ? | - |
? | |
D-galactonate | substrate specificity 14% | Achromobacter xylosoxidans | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
D-gluconate | substrate specificity 100% | Achromobacter xylosoxidans | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
D-mannonate | substrate specificity 13% | Achromobacter xylosoxidans | ? | - |
? | |
D-ribonate | substrate specificity 11% | Achromobacter xylosoxidans | ? | - |
? | |
D-xylonate | substrate specificity 82% | Achromobacter xylosoxidans | ? | - |
? | |
DL-isovalerate | substrate specificity 11% | Achromobacter xylosoxidans | ? | - |
? | |
L-arabonate | substrate specificity 2% | Achromobacter xylosoxidans | ? | - |
? | |
L-mannonate | substrate specificity 10% | Achromobacter xylosoxidans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Achromobacter xylosoxidans |
Synonyms | Comment | Organism |
---|---|---|
D-gluconate dehydratase | - |
Achromobacter xylosoxidans |
gluconate dehydratase | - |
Achromobacter xylosoxidans |
GNAD | - |
Achromobacter xylosoxidans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
standard assay condition | Achromobacter xylosoxidans |
50 | - |
optimal activity | Achromobacter xylosoxidans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Achromobacter xylosoxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
standard assay condition | Achromobacter xylosoxidans |
8.5 | - |
optimal activity | Achromobacter xylosoxidans |