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D-galactoheptonate
2-dehydro-3-deoxy-D-galactoheptonate + H2O
-
Substrates: 1.6% of the activity with D-gluconate
Products: -
?
D-galactonate
2-dehydro-3-deoxy-D-galactonate + H2O
D-galactonate
?
-
Substrates: 85% of activity with D-gluconate
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
D-ribonate
?
-
Substrates: substrate specificity 11%
Products: -
?
DL-isovalerate
?
-
Substrates: substrate specificity 11%
Products: -
?
gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
Substrates: -
Products: -
?
L-mannonate
?
-
Substrates: substrate specificity 10%
Products: -
?
additional information
?
-
D-arabonate
?
-
Substrates: substrate specificity 4%
Products: -
?
D-arabonate
?
-
Substrates: 44% of activity with D-gluconate
Products: -
?
D-fuconate
?
-
Substrates: substrate specificity 18%
Products: -
?
D-fuconate
?
-
Substrates: 15% of activity with D-gluconate
Products: -
?
D-fuconate
?
-
Substrates: 15% of activity with D-gluconate
Products: -
?
D-galactonate
2-dehydro-3-deoxy-D-galactonate + H2O
-
Substrates: substrate specificity 14%
Products: -
?
D-galactonate
2-dehydro-3-deoxy-D-galactonate + H2O
Substrates: low activity
Products: -
?
D-galactonate
2-dehydro-3-deoxy-D-galactonate + H2O
Substrates: low activity
Products: -
?
D-galactonate
2-dehydro-3-deoxy-D-galactonate + H2O
Substrates: -
Products: -
?
D-galactonate
2-dehydro-3-deoxy-D-galactonate + H2O
-
Substrates: 2.8% of the activity with D-gluconate. Studies by other groups reveal a catalytic efficiency of 20% for D-galactonate compared to D-gluconate
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate
-
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate
-
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate
-
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: substrate specificity 100%
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: key enzyme in the non-phosphorylated Entner-Doudoroff pathway
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
Substrates: no activity with D-galactopnate and D-xylonate
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
Substrates: no activity with D-galactopnate and D-xylonate
Products: -
?
D-gluconate
?
-
Substrates: first enzyme in modified Entner-Doudoroff pathway
Products: -
?
D-gluconate
?
-
Substrates: enzyme only active during growth on gluconate
Products: -
?
D-gluconate
?
-
Substrates: enzyme only active during growth on gluconate
Products: -
?
D-gluconate
?
-
Substrates: -
Products: -
?
D-gluconate
?
-
Substrates: first enzyme in modified Entner-Doudoroff pathway
Products: -
?
D-gluconate
?
-
Substrates: enzyme only active during growth on gluconate
Products: -
?
D-gluconate
?
-
Substrates: involved in conventional Entner-Doudoroff pathway for gluconate utilization
Products: -
?
D-gluconate
?
-
Substrates: enzyme only active during growth on gluconate
Products: -
?
D-gluconate
?
-
Substrates: first enzyme in modified Entner-Doudoroff pathway
Products: -
?
D-gluconate
?
-
Substrates: first enzyme in modified Entner-Doudoroff pathway
Products: -
?
D-gluconate
?
-
Substrates: first enzyme in modified Entner-Doudoroff pathway
Products: -
?
D-gluconate
?
-
Substrates: involved in glucose metabolism
Products: -
?
D-mannonate
?
-
Substrates: substrate specificity 13%
Products: -
?
D-mannonate
?
-
Substrates: 16% of activity with D-gluconate
Products: -
?
D-mannonate
?
-
Substrates: 74% of activity with D-gluconate
Products: -
?
D-xylonate
?
-
Substrates: substrate specificity 82%
Products: -
?
D-xylonate
?
-
Substrates: 89% of activity with D-gluconate
Products: -
?
D-xylonate
?
-
Substrates: 89% of activity with D-gluconate
Products: -
?
L-arabonate
?
-
Substrates: substrate specificity 2%
Products: -
?
L-arabonate
?
-
Substrates: 49% of activity with D-gluconate
Products: -
?
additional information
?
-
-
Substrates: specific for aldonic acids with 5 or 6 carbon atoms, having hydroxyl groups at C-2 and C-3 in L-threo configuration, no activity with various other compounds tested
Products: -
?
additional information
?
-
-
Substrates: specific for aldonic acids with 5 or 6 carbon atoms, having hydroxyl groups at C-2 and C-3 in L-threo configuration, no activity with various other compounds tested
Products: -
?
additional information
?
-
-
Substrates: -
Products: -
?
additional information
?
-
-
Substrates: only substrate is D-gluconate, not: D-galactonate, D-galactarate, D-galacturonate, 6-phospho-D-gluconate, D-arabonate, D-xylonate, L-mannonate, D-glucoheptonate
Products: -
?
additional information
?
-
Substrates: the enzyme catalyzes the specific dehydration of gluconate and does not utilize galactonate at significant rates
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyzes the specific dehydration of gluconate and does not utilize galactonate at significant rates
Products: -
?
additional information
?
-
Substrates: the enzyme catalyzes the specific dehydration of gluconate and does not utilize galactonate at significant rates
Products: -
?
additional information
?
-
Substrates: xylonate (10 mM) is not utilized
Products: -
?
additional information
?
-
-
Substrates: xylonate (10 mM) is not utilized
Products: -
?
additional information
?
-
-
Substrates: gluconate dehydratase being active only with C6 metabolites
Products: -
?
additional information
?
-
-
Substrates: gluconate dehydratase being active only with C6 metabolites
Products: -
?
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D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
additional information
?
-
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: substrate specificity 100%
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: key enzyme in the non-phosphorylated Entner-Doudoroff pathway
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: -
Products: -
?
D-gluconate
2-dehydro-3-deoxy-D-gluconate + H2O
-
Substrates: -
Products: -
?
D-gluconate
?
-
Substrates: first enzyme in modified Entner-Doudoroff pathway
Products: -
?
D-gluconate
?
-
Substrates: enzyme only active during growth on gluconate
Products: -
?
D-gluconate
?
-
Substrates: enzyme only active during growth on gluconate
Products: -
?
D-gluconate
?
-
Substrates: -
Products: -
?
D-gluconate
?
-
Substrates: first enzyme in modified Entner-Doudoroff pathway
Products: -
?
D-gluconate
?
-
Substrates: enzyme only active during growth on gluconate
Products: -
?
D-gluconate
?
-
Substrates: involved in conventional Entner-Doudoroff pathway for gluconate utilization
Products: -
?
D-gluconate
?
-
Substrates: enzyme only active during growth on gluconate
Products: -
?
D-gluconate
?
-
Substrates: first enzyme in modified Entner-Doudoroff pathway
Products: -
?
D-gluconate
?
-
Substrates: first enzyme in modified Entner-Doudoroff pathway
Products: -
?
D-gluconate
?
-
Substrates: first enzyme in modified Entner-Doudoroff pathway
Products: -
?
D-gluconate
?
-
Substrates: involved in glucose metabolism
Products: -
?
additional information
?
-
-
Substrates: gluconate dehydratase being active only with C6 metabolites
Products: -
?
additional information
?
-
-
Substrates: gluconate dehydratase being active only with C6 metabolites
Products: -
?
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Cd2+
the enzyme is activated by the presence of Co2+, Mg2+, Ni2+, Cd2+
Co2+
-
Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace
Co2+
-
Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace
Co2+
-
200% activation at 30 mM
Co2+
-
1 mM, activates. Presence of one Co2+ per enzyme monomer
Co2+
the enzyme is activated by the presence of Co2+, Mg2+, Ni2+, Cd2+
Fe2+
-
Fe2+ and sulfhydryl compound required
Fe2+
-
at 2 mM most effective, activates and reactivates after O2 inactivation, probably two distinct sites for activation and reactivation, Mn2+, Co2+, Mg2+ can partially replace
Mg2+
-
Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace
Mg2+
-
100% activation at 1 mM
Mg2+
-
Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace
Mg2+
-
Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace
Mg2+
the enzyme is activated by the presence of Co2+, Mg2+, Ni2+, Cd2+
Mn2+
-
50% activation at 0.1 mM, 30% activation at 1 mM
Mn2+
-
Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace
Mn2+
-
Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace
Mn2+
-
Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace
Mn2+
-
40% activation at 30 mM
Ni2+
-
1 mM, activates
Ni2+
the enzyme is activated by the presence of Co2+, Mg2+, Ni2+, Cd2+
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Bender, R.; Gottschalk, G.
Purification and properties of D-gluconate dehydratase from Clostridium pasteurianum
Eur. J. Biochem.
40
309-321
1973
Clostridium pasteurianum
brenda
Kersters, K.; De Ley, J.
D-Gluconate dehydratase from Alcaligenes
Methods Enzymol.
42
301-304
1975
Agrobacterium tumefaciens, Alcaligenes sp., Alcaligenes sp. M250, Aspergillus niger, Cereibacter sphaeroides, Clostridium pasteurianum, Clostridium sp.
brenda
Gottschalk, G.; Bender, R.
D-gluconate dehydratase from Clostridium pasteurianum
Methods Enzymol.
90
283-287
1982
Clostridium pasteurianum
brenda
Bender, R.; Gottschalk, G.
Enzymatic synthesis of 2-keto-3-deoxy-D-gluconate from D-gluconate
Anal. Biochem.
61
275-279
1974
Clostridium pasteurianum
brenda
Andreesen, J.R.; Gottschalk, G.
The occurence of a modified Entner-Doudoroff pathway in Clostridium aceticum
Arch. Microbiol.
69
160-170
1969
Clostridium aceticum
brenda
Bender, R.; Andreesen, J.R.; Gottschalk, G.
2-Keto-3-deoxygluconate, an intermediate in the fermentation of gluconate by clostridia
J. Bacteriol.
107
570-573
1971
Clostridium beijerinckii, Clostridium butyricum, Clostridium pasteurianum, Clostridium roseum, Clostridium tetanomorphum
brenda
Tomlinson, G.A.; Koch, T.K.
The metabolism of carbohydrates by extremely halophilic bacteria. Glucose metabolism via a modifiedf Entner-Doudoroff pathway
Can. J. Microbiol.
20
1085-1091
1973
halophilic archaeon, halophilic archaeon M6
brenda
Bugden, N.; Danson, M.J.
Metabolism of glucose via a modified Entner-Doudoroff pathway in the thermoacidophilic archaebacterium Thermoplasma acidophilum
FEBS Lett.
196
207-210
1986
Thermoplasma acidophilum
-
brenda
Green, P.N.; Gibson, D.M.
Carbohydrate metabolism in some methylotrophic bacteria
FEMS Microbiol. Lett.
23
31-34
1984
Methylobacterium mesophilicum
-
brenda
Pitt, D.; Mosley, M.J.
Enzymes of gluconate metabolism and glycolysis in Penicillium notatum
Antonie van Leeuwenhoek
51
353-364
1985
Penicillium chrysogenum
brenda
Zablotny, R.; Fraenkel, D.G.
Glucose and gluconate metabolism in a mutant of Escherichia coli lacking gluconate-6-phosphate dehydrase
J. Bacteriol.
93
1579-1581
1967
Escherichia coli
brenda
Elshafel, A.M.
Temperature-dependent behaviour of enzymes of D-gluconate catabolism in Penicillium species
Egypt. J. Microbiol.
2
167-171
1987
Penicillium chrysogenum, Penicillium oxalicum
-
brenda
Hassan, M.M.; Hosseny, E.N.; Elzainy, T.A.; Allam, A.M.
Some properties of D-gluconate dehydratase from Penicillium citrinum
Egypt. J. Bot.
29/30
211-220
1986
Penicillium citrinum
-
brenda
Kim, S.; Lee, S.B.
Identification and characterization of Sulfolobus solfataricus D-gluconate dehydratase: a key enzyme in the non-phosphorylated Entner-Doudoroff pathway
Biochem. J.
387
271-280
2005
Saccharolobus solfataricus
brenda
Kim, S.; Lee, S.B.
Identification and characterization of the bacterial D-gluconate dehydratase in Achromobacter xylosoxidans
Biotechnol. Bioprocess Eng.
13
436-444
2008
Achromobacter xylosoxidans
-
brenda
Kim, S.; Lee, S.B.
Soluble expression of archaeal proteins in Escherichia coli by using fusion-partners
Protein Expr. Purif.
62
116-119
2008
Saccharolobus solfataricus
brenda
Reher, M.; Fuhrer, T.; Bott, M.; Schoenheit, P.
The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate-specific aldolase
J. Bacteriol.
192
964-974
2010
Picrophilus torridus (Q6L1T2), Picrophilus torridus
brenda
Nunn, C.E.; Johnsen, U.; Schoenheit, P.; Fuhrer, T.; Sauer, U.; Hough, D.W.; Danson, M.J.
Metabolism of pentose sugars in the hyperthermophilic archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius
J. Biol. Chem.
285
33701-33709
2010
Saccharolobus solfataricus, Saccharolobus solfataricus P2
brenda
Matsubara, K.; Khling, R.; Schnenberger, B.; Kouril, T.; Esser, D.; Brsen, C.; Siebers, B.; Wohlgemuth, R.
One-step synthesis of 2-keto-3-deoxy-D-gluconate by biocatalytic dehydration of D-gluconate
J. Biotechnol.
191
69-77
2014
Thermoproteus tenax (G4RJQ1), Thermoproteus tenax, Thermoproteus tenax Kra 1 (G4RJQ1)
brenda
Wichelecki, D.J.; Balthazor, B.M.; Chau, A.C.; Vetting, M.W.; Fedorov, A.A.; Fedorov, E.V.; Lukk, T.; Patskovsky, Y.V.; Stead, M.B.; Hillerich, B.S.; Seidel, R.D.; Almo, S.C.; Gerlt, J.A.
Discovery of function in the enolase superfamily: D-mannonate and D-gluconate dehydratases in the D-mannonate dehydratase subgroup
Biochemistry
53
2722-2731
2014
Salmonella enterica subsp. enterica serovar Enteritidis, Salmonella enterica subsp. enterica serovar Enteritidis P125109
brenda
Sutter, J.M.; Taestensen, J.B.; Johnsen, U.; Soppa, J.; Schoenheit, P.
Key enzymes of the semiphosphorylative Entner-Doudoroff pathway in the haloarchaeon Haloferax volcanii characterization of glucose dehydrogenase, gluconate dehydratase, and 2-keto-3-deoxy-6-phosphogluconate aldolase
J. Bacteriol.
198
2251-2262
2016
Haloferax volcanii (D4GYD4), Haloferax volcanii, Haloferax volcanii DSM 3757 (D4GYD4)
brenda
Matsubara, K.; Koehling, R.; Schoenenberger, B.; Kouril, T.; Esser, D.; Braesen, C.; Siebers, B.; Wohlgemuth, R.
One-step synthesis of 2-keto-3-deoxy-D-gluconate by biocatalytic dehydration of D-gluconate
J. Biotechnol.
191
69-77
2014
Thermoproteus tenax (G4RJQ1), Thermoproteus tenax, Thermoproteus tenax DSM 2078 (G4RJQ1)
brenda