BRENDA - Enzyme Database
show all sequences of 4.1.2.51

The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate-specific aldolase

Reher, M.; Fuhrer, T.; Bott, M.; Schoenheit, P.; J. Bacteriol. 192, 964-974 (2010) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Picrophilus torridus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
0.4
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
2.5
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
2.7
-
pyruvate
pH 6.2, 60°C
Picrophilus torridus
4.6
-
D-glyceraldehyde
pH 6.2, 60°C
Picrophilus torridus
8
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
4 * 32000, SDS-PAGE
Picrophilus torridus
120000
-
gel filtration
Picrophilus torridus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-dehydro-3-deoxy-D-gluconate
Picrophilus torridus
the enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway
pyruvate + D-glyceraldehyde
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Picrophilus torridus
Q6KZI8
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Picrophilus torridus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
48
-
pH 6.2, 60°C, substrate: 2-dehydro-3-deoxy-D-gluconate
Picrophilus torridus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-dehydro-3-deoxy-D-galactonate
-
704334
Picrophilus torridus
pyruvate + D-glyceraldehyde
-
-
-
?
2-dehydro-3-deoxy-D-galactonate
the enzyme shows substrate promiscuity, as measured in the direction of aldol synthesis. The enzyme catalyzes the formation of both 2-dehydro-3-deoxy-D-gluconate and its C-4 epimer 2-keto-3-deoxygalactonate from D- or L-glyceraldehyde and pyruvate at a similar ratio, indicating lack of stereospecific control in formation and cleavage of 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate
704334
Picrophilus torridus
pyruvate + D-glyceraldehyde
-
-
-
r
2-dehydro-3-deoxy-D-gluconate
the enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway
704334
Picrophilus torridus
pyruvate + D-glyceraldehyde
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold-higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate
704334
Picrophilus torridus
pyruvate + D-glyceraldehyde
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold-higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate
704334
Picrophilus torridus
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 32000, SDS-PAGE
Picrophilus torridus
Synonyms
Synonyms
Commentary
Organism
KDG-specific aldolase
-
Picrophilus torridus
KDGA
-
Picrophilus torridus
Pto1279
-
Picrophilus torridus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Picrophilus torridus
65
-
-
Picrophilus torridus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
2 h, stable
Picrophilus torridus
80
-
half-life: 20 min
Picrophilus torridus
90
-
half-life: 15 min
Picrophilus torridus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.34
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
0.36
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
24.2
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
26.7
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
-
Picrophilus torridus
6.2
-
assay at
Picrophilus torridus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
7.5
pH 4.5: 50% of maximal activity, pH 7.5: about 50% of maximal activity
Picrophilus torridus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Picrophilus torridus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
0.4
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
2.5
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
2.7
-
pyruvate
pH 6.2, 60°C
Picrophilus torridus
4.6
-
D-glyceraldehyde
pH 6.2, 60°C
Picrophilus torridus
8
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
4 * 32000, SDS-PAGE
Picrophilus torridus
120000
-
gel filtration
Picrophilus torridus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-dehydro-3-deoxy-D-gluconate
Picrophilus torridus
the enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway
pyruvate + D-glyceraldehyde
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Picrophilus torridus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
48
-
pH 6.2, 60°C, substrate: 2-dehydro-3-deoxy-D-gluconate
Picrophilus torridus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-dehydro-3-deoxy-D-galactonate
-
704334
Picrophilus torridus
pyruvate + D-glyceraldehyde
-
-
-
?
2-dehydro-3-deoxy-D-galactonate
the enzyme shows substrate promiscuity, as measured in the direction of aldol synthesis. The enzyme catalyzes the formation of both 2-dehydro-3-deoxy-D-gluconate and its C-4 epimer 2-keto-3-deoxygalactonate from D- or L-glyceraldehyde and pyruvate at a similar ratio, indicating lack of stereospecific control in formation and cleavage of 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate
704334
Picrophilus torridus
pyruvate + D-glyceraldehyde
-
-
-
r
2-dehydro-3-deoxy-D-gluconate
the enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway
704334
Picrophilus torridus
pyruvate + D-glyceraldehyde
-
-
-
?
2-dehydro-3-deoxy-D-gluconate
highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold-higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate
704334
Picrophilus torridus
pyruvate + D-glyceraldehyde
-
-
-
?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold-higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate
704334
Picrophilus torridus
pyruvate + D-glyceraldehyde 3-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 32000, SDS-PAGE
Picrophilus torridus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Picrophilus torridus
65
-
-
Picrophilus torridus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
2 h, stable
Picrophilus torridus
80
-
half-life: 20 min
Picrophilus torridus
90
-
half-life: 15 min
Picrophilus torridus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.34
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
0.36
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
24.2
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
26.7
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
-
Picrophilus torridus
6.2
-
assay at
Picrophilus torridus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
7.5
pH 4.5: 50% of maximal activity, pH 7.5: about 50% of maximal activity
Picrophilus torridus
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in the nonphosphorylative variant of the Entner–Doudoroff pathway
Picrophilus torridus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in the nonphosphorylative variant of the Entner–Doudoroff pathway
Picrophilus torridus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.04
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
0.14
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
52
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
89
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.04
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
0.14
-
2-dehydro-3-deoxy-D-gluconate 6-phosphate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
52
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, recombinant enzyme
Picrophilus torridus
89
-
2-dehydro-3-deoxy-D-gluconate
pH 6.2, 60°C, enzyme purified from Picrophilus torridus
Picrophilus torridus
Other publictions for EC 4.1.2.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
704334
Reher
The nonphosphorylative Entner- ...
Picrophilus torridus
J. Bacteriol.
192
964-974
2010
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