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Literature summary for 4.1.2.45 extracted from
- Presence of two trans-o-hydroxybenzylidenepyruvate hydratase-aldolases in naphthalenesulfonate-assimilating Sphingomonas paucimobilis TA-2: comparison of some properties (2000), J. Biochem., 127, 43-49.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 1 mM, 40% inhibition of fHBP HA B | Sphingomonas paucimobilis |  |
| additional information | no inhibition: monoiodoacetic acid, N-ethylmaleimide, PCMB, diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, EDTA, NaN3, KCN, o-phenanthroline, 2,2'-bipyridine, Fe2+, Fe3+, Mg2+, Mn2+, Co2+, Ca2+, Zn2+, Cd2+, Cu2+, Ni2+, EDTA, o-phenanthroline, 2'-bipyridine, KCN | Sphingomonas paucimobilis | |
| NEM | 1 mM, 30 min, 25°C, 35% inhibition of fHBP HA B, more than 85% of the initial activity of fHBP HA A remains | Sphingomonas paucimobilis | |
| PCMB | 1 mM, 30 min, 25°C, complete inhibition of fHBP HA B, more than 85% of the initial activity of fHBP HA A remains | Sphingomonas paucimobilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate | 30°C, pH 8.1, enzyme form fHBP HA A | Sphingomonas paucimobilis | |
| 0.009 | - |
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate | 30°C, pH 8.1, enzyme form fHBP HA B | Sphingomonas paucimobilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37200 | - |
3 * 37200, enzyme form fHBP HA B, SDS-PAGE | Sphingomonas paucimobilis |
| 37600 | - |
3 * 37600, enzyme form fHBP HA A, SDS-PAGE | Sphingomonas paucimobilis |
| 104000 | - |
enzyme form fHBP HA B, ultracentrifugation | Sphingomonas paucimobilis |
| 116000 | - |
enzyme form fHBP HA A, ultracentrifugation | Sphingomonas paucimobilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingomonas paucimobilis | - |
formerly Pseudomonas sp. TA-2 | - |
| Sphingomonas paucimobilis TA-2 | - |
formerly Pseudomonas sp. TA-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| presence of at least three trans-o-hydroxybenzylidenepyruvate hydratase-aldolases, purification of two typical trans-o-hydroxybenzylidenepyruvate hydratase-aldolases (fHBP HA A and fHBP HA B) | Sphingomonas paucimobilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
enzyme form fHBP HA B | Sphingomonas paucimobilis |
| 11 | - |
enzyme form fHBP HA A | Sphingomonas paucimobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O | i.e. 2'-hydroxybenzylidinepyruvate, i.e. 2'-hydroxybenzalpyruvate | Sphingomonas paucimobilis | salicylaldehyde + pyruvate | - |
? | |
| (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O | i.e. 2'-hydroxybenzylidinepyruvate, i.e. 2'-hydroxybenzalpyruvate | Sphingomonas paucimobilis TA-2 | salicylaldehyde + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 37200, enzyme form fHBP HA B, SDS-PAGE | Sphingomonas paucimobilis |
| trimer | 3 * 37600, enzyme form fHBP HA A, SDS-PAGE | Sphingomonas paucimobilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fHBP HA A | - |
Sphingomonas paucimobilis |
| fHBP HA B | - |
Sphingomonas paucimobilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 7.3, 20 mM Tris-HCl buffer, 5 min, enzyme form fHBP HA A and enzyme form fHBP HA B, stable up to | Sphingomonas paucimobilis |
| 65 | - |
pH 7.3, 20 mM Tris-HCl buffer, 5 min, enzyme form fHBP HA A and enzyme form fHBP HA B, 80% loss of activity | Sphingomonas paucimobilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
enzyme form fHBP HA A and enzyme form fHBP HA B | Sphingomonas paucimobilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | pH 6: about 60% of maximal activity, pH 9.5: about 80% of maximal activity, enzyme form fHBP HA A and enzyme form fHBP HA B | Sphingomonas paucimobilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.3 | 4°C, 20 h, enzyme form fHBP HA A is stable | Sphingomonas paucimobilis |
| 7.1 | 10.7 | 4°C, 20 h, enzyme form fHBP HA B is stable | Sphingomonas paucimobilis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sphingomonas paucimobilis | enzyme form fHBP HA B, isoelectric focusing | - |
4.3 |
| Sphingomonas paucimobilis | enzyme form fHBP HA A, isoelectric focusing | - |
4.8 |
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