General Stability | Organism |
---|---|
graduall decrease in activity by repeated freezing and thawing | Sus scrofa |
no loss of activity after lyophilization | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3,4-Trihydroxybenzylhydrazine | - |
Sus scrofa | |
Amino-oxyacetate | - |
Sus scrofa | |
BH4 | - |
Sus scrofa | |
Dithiobisnitrobenzoate | - |
Sus scrofa | |
DL-alpha-Difluoromethyl-beta-(3,4-dihydroxyphenyl)alanine | - |
Sus scrofa | |
DL-alpha-Monofluoromethyl-beta-(3,4-dihydroxyphenyl)alanine | - |
Sus scrofa | |
hydroxylamine | - |
Sus scrofa | |
iodoacetamide | - |
Sus scrofa | |
L-alpha-Methyl-alpha-hydrazino-3,4-dihydroxyphenylpropionic acid | - |
Sus scrofa | |
mercuribenzoate | - |
Sus scrofa | |
NEM | - |
Sus scrofa | |
Semicarbazide | - |
Sus scrofa | |
Thiosemicarbazide | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-o-Tyr | - |
Sus scrofa | |
0.18 | - |
3,4-dihydroxyphenylalanine | - |
Sus scrofa | |
0.23 | - |
5-hydroxytryptophan | - |
Sus scrofa | |
0.52 | - |
L-m-Tyr | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Sus scrofa |
103000 | - |
ultracentrifugation analysis | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, 0.1 M potassium phosphate buffer, 0.1 mM dithiothreitol, stable for at least 2 months | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-Dihydroxyphenylalanine | - |
Sus scrofa | 3-Hydroxytyramine + CO2 | - |
? | |
5-Hydroxytryptophan | - |
Sus scrofa | Serotonin + CO2 | - |
? | |
m-Tyr | - |
Sus scrofa | ? | - |
? | |
o-Tyr | - |
Sus scrofa | ? | - |
? | |
Tyr | - |
Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 1 mol of pyridoxal 5'-phosphate is bound to the epsilon-amino group of a lysyl residue of the apoenzyme | Sus scrofa |