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Literature summary for 3.6.5.2 extracted from

  • Kaiser, A.; Langer, B.; Przyborski, J.; Kersting, D.; Krueger, M.
    A putative non-canonical Ras-like GTPase from P. falciparum chemical properties and characterization of the protein (2015), PLoS ONE, 10, e0140994 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.2

Activating Compound

Activating Compound Comment Organism Structure
mastoparan stimulates GTP binding in the presence of Mg2+ and guanine nucleotide exchange, mastoparan mimics the GPCR mode of action Plasmodium falciparum
Sodium fluoride stimulates of guanine nucleotide exchange, based on the formation of a fluoroaluminate complex which can pass the membrane and occupy the position next to bound GDP and thus stimulates an active state conformation Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
gene PfG, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR enzyme expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta, subcloning in Escherichia coli strain NEB 5-alpha Plasmodium falciparum

General Stability

General Stability Organism
the purified recombinant His6-tagged enzyme is stable only for 3.5 hours Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol parasite cytosol Plasmodium falciparum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
108570
-
 sequence calculation Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Plasmodium falciparum
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum O77338
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta by nickel affinity chromatography and blue native PAGE Plasmodium falciparum

Source Tissue

Source Tissue Comment Organism Textmining
gametocyte
-
 Plasmodium falciparum
-
additional information transcript levels and expression of the enzyme are significantly increased in the erythrocytic phase in particular during schizont and gametocyte formation Plasmodium falciparum
-
schizont
-
 Plasmodium falciparum
-
trophozoite low content in late trophozoites, very low in early trophozoites Plasmodium falciparum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Plasmodium falciparum GDP + phosphate
-
 ?
additional information no activity with unhydrolyzable GTPgammaS, which is used for association/dissociation and binding analysis Plasmodium falciparum ?
-
-

Synonyms

Synonyms Comment Organism
non-canonical Ras-like G protein
-
 Plasmodium falciparum
PfG
-
 Plasmodium falciparum
Ras-like G-protein
-
 Plasmodium falciparum
Ras-like GTpase
-
 Plasmodium falciparum

pI Value

Organism Comment pI Value Maximum pI Value
Plasmodium falciparum sequence calculation
-
 8.68

General Information

General Information Comment Organism
additional information the enzyme has has GTP binding capacity and GTPase activity due to an EngA2 domain present in small Ras-like GTPases Plasmodium falciparum
physiological function the putitative, Ras-like G-protein might be involved in a non-canonical signaling pathway in Plasmodium falciparum Plasmodium falciparum