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Literature summary for 3.6.5.2 extracted from

  • Zhang, L.; Dai, F.; Cui, L.; Zhou, B.; Guo, Y.
    Up-regulation of the active form of small GTPase Rab13 promotes macroautophagy in vascular endothelial cells (2017), Biochim. Biophys. Acta, 1864, 613-624 .
    View publication on PubMed
Search for more literature for 3.6.5.2

Cloned(Commentary)

Cloned (Comment) Organism
gene RAB13, semiquantitative RT-PCR and quantitative real-time PCR expression analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information knockdown of Rab13, phenotype, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
autophagosome
-
 Homo sapiens 5776
-
cell surface
-
 Homo sapiens 9986
-
cytoplasm
-
 Homo sapiens 5737
-
additional information pterostilbene evokes redistribution of Rab13 from the cell surface and diffuse cytoplasmic location to exclusively cytoplasmic, punctuated autophagosomes as shown by Rab13 co-localization with LC3 in HUVECs Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P51153
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
HUVEC cell
-
 Homo sapiens
-
vascular endothelial cell HUVECs Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Rab13
-
 Homo sapiens
small GTPase
-
 Homo sapiens
small GTPase Ras-related protein in brain 13
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens Rab13 is the most significantly upregulated gene in pterostilbene-treated human umbilical VECs (HUVECs) up

General Information

General Information Comment Organism
malfunction knockdown of Rab13 blocks pterostilbene-induced mTOR inhibition and autophagy, whereas overexpression of the GTP-containing active form of Rab13 induces mTOR inhibition and autophagy in HUVECs. Upregulation of the active form of small GTPase Rab13 promotes macroautophagy in vascular endothelial cells. Knockdown of Grb2 suppresses pterostilbene or upregulation of the active form of Rab13-induced autophagy. Knockdown of Rab13 inhibits the digestion of cytoplasmic components in pterostilbene-treated HUVECs numerous autophagosome- or autolysosome-like vesicular structures Homo sapiens
metabolism an increasing number of Rab GTPases have been shown to play either critical or accessory roles in various stages of autophagy. Rab1, Rab4, Rab5, Rab11, Rab12, Rab17, Rab23, Rab26, Rab30 and Rab32 participate in autophagosome formation. Rab13 is required for pterostilbene-induced autophagy Homo sapiens
physiological function Macroautophagy (i.e. autophagy) a highly conserved intracellular process by which cytosolic components and damaged organelles are enclosed and degraded by a double membrane-bound structure, can be viewed as a continuous and dynamic membrane transport and fusion process. The GTP-bound active form of Rab13 promotes the interaction between Rab13 and growth factor receptor-bound protein 2 (Grb2). It promotes macroautophagy in vascular endothelial cells. Rab13 activates the downstream AMP-activated protein kinase (AMPK) and blocks mammalian target of rapamycin (mTOR) signaling by its functional interaction with Grb2 to regulate autophagy in HUVECs. Rab13 is a regulator of autophagy in VECs under nutrient-enriched conditions. Upregulation of the active form of Rab13 increases its interaction with Grb2, which is required to activate AMPK, leading to the inhibition of mTOR and subsequently inducing autophagy. Rab13 regulated autophagy by interacting with Grb2 in HUVECs Homo sapiens