Inhibitors | Comment | Organism | Structure |
---|---|---|---|
I2 | - |
Streptomyces cacaoi | |
N-bromosuccinimide | - |
Streptomyces cacaoi | |
p-chloromercuribenzoate | week | Streptomyces cacaoi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
ampicillin | - |
Streptomyces cacaoi | |
0.063 | - |
benzylpenicillin | - |
Streptomyces cacaoi | |
0.065 | - |
carbenicillin | - |
Streptomyces cacaoi | |
0.087 | - |
2,2-dimethyl-6beta-({[5-methyl-3-(2-chlorophenyl)isoxazol-4-yl]carbonyl}amino)penam-3alpha-carboxylic acid | - |
Streptomyces cacaoi | |
0.131 | - |
Methicillin | - |
Streptomyces cacaoi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Streptomyces cacaoi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cacaoi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces cacaoi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2430 | - |
- |
Streptomyces cacaoi |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 6 months | Streptomyces cacaoi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | 38% relative activity to benzylpenicillin | Streptomyces cacaoi | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | 100% relative activity to other substrates tested | Streptomyces cacaoi | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
carbenicillin + H2O | 66% relative activity to benzylpenicillin | Streptomyces cacaoi | (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cephaloridine + H2O | 3% relative activity to benzylpenicillin | Streptomyces cacaoi | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephalothin + H2O | 1% relative activity to benzylpenicillin | Streptomyces cacaoi | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cloxacillin + H2O | 38% relative activity to benzylpenicillin | Streptomyces cacaoi | (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
methicillin + H2O | 73% relative activity | Streptomyces cacaoi | (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Streptomyces cacaoi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
unstable above 50°C | Streptomyces cacaoi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
473 | - |
benzylpenicillin | - |
Streptomyces cacaoi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Streptomyces cacaoi |