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Literature summary for 3.4.24.56 extracted from

  • Kurochkin, I.V.; Guarnera, E.; Wong, J.H.; Eisenhaber, F.; Berezovsky, I.N.
    Toward allosterically increased catalytic activity of insulin-degrading enzyme against amyloid peptides (2017), Biochemistry, 56, 228-239 .
    View publication on PubMed
Search for more literature for 3.4.24.56

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D426A mutation diminishes activity Homo sapiens
F807A mutation decreass the Km-value of the amyloid beta substrate Homo sapiens
K364A mutation does not change the activity Homo sapiens
K898A mutation decreass the Km-value of the amyloid beta substrate Homo sapiens
K898A mutation results in increased catalytic activity Homo sapiens
S137A mutation decreass the Km-value of the amyloid beta substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, wild-type enzyme Homo sapiens
0.035
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme K364A Homo sapiens
0.035
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme S137A Homo sapiens
0.043
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme K898A Homo sapiens
0.044
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme F807A Homo sapiens
0.103
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme D426A Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amyloid beta + H2O Homo sapiens role of insulin-degrading enzyme in the intracytosolic clearance of amyloid beta and other amyloid-like peptides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P14735
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-GGFLRKHGQ-EDDnp + H2O
-
 Homo sapiens ?
-
 ?
amyloid beta + H2O
-
 Homo sapiens ?
-
 ?
amyloid beta + H2O role of insulin-degrading enzyme in the intracytosolic clearance of amyloid beta and other amyloid-like peptides Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
IDE
-
 Homo sapiens
Insulin-degrading enzyme
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme D426A Homo sapiens
1.68
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme F807A Homo sapiens
1.7
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, wild-type enzyme Homo sapiens
1.83
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme K364A Homo sapiens
1.97
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme S137A Homo sapiens
2.42
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme K898A Homo sapiens

General Information

General Information Comment Organism
physiological function role of insulin-degrading enzyme in the intracytosolic clearance of amyloid beta and other amyloid-like peptides Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.97
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme D426A Homo sapiens
38.18
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme F807A Homo sapiens
51.5
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, wild-type enzyme Homo sapiens
52.3
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme K364A Homo sapiens
56.3
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme K898A Homo sapiens
56.3
-
 Abz-GGFLRKHGQ-EDDnp pH 7.7, 37°C, mutant enzyme S137A Homo sapiens