Literature summary for 3.4.22.49 extracted from

Haass, W.; Kleiner, H.; Mueller, M.C.; Hofmann, W.K.; Fabarius, A.; Seifarth, W.
Measurement of separase proteolytic activity in single living cells by a fluorogenic flow cytometry assay (2015), PLoS ONE, 10, e0133769 .

Search for more literature for 3.4.22.49

Application

Application	Comment	Organism
diagnostics	measurement of separase proteolytic activity in single living cells by a fluorogenic flow cytometry assay. The assay is used to quantify Separase proteolytic activity in leukemic cell lines and peripheral blood samples from leukemia patients	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q14674	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
U-937 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[Cy5-D-R-E-I-M-R]2-rhodamine-110 + H2O	-	Homo sapiens	2 [Cy5-D-R-E-I-M-R] + rhodamine-110	-	?

General Information

General Information	Comment	Organism
malfunction	overexpression and deregulated proteolytic activity of Separase as frequently observed in human cancers is associated with the occurrence of supernumerary centrosomes, chromosomal missegregation and aneuploidy	Homo sapiens
physiological function	the enzyme is required for centrosome duplication and separation of sister-chromatides in anaphase of mitosis	Homo sapiens

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Release 2023.1 (January 2023)