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Literature summary for 3.4.21.83 extracted from

  • Mohamed Mustafa, M.S.; Nakajima, Y.; Oyama, H.; Iwata, N.; Ito, K.
    Assessment of substrate inhibition of bacterial oligopeptidase B (2012), Biol. Pharm. Bull., 35, 2010-2016.
    View publication on PubMed
Search for more literature for 3.4.21.83

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli
expression in Escherichia coli Stenotrophomonas maltophilia
expression in Escherichia coli Rhodococcus erythropolis
expression in Escherichia coli Serratia marcescens

Organism

Organism UniProt Comment Textmining
Escherichia coli P24555
-
-
Rhodococcus erythropolis C0ZRJ2
-
-
Serratia marcescens K0J8D4
-
-
Stenotrophomonas maltophilia K0J2A4
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5
-
 substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
6
-
 substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
6
-
 substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
6
-
 substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
13
-
 substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
18
-
 substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
42 60 substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
53
-
 substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
86
-
 substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
86
-
 substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
90
-
 substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
100
-
 substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
105
-
 substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
124
-
 substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
126
-
 substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
158
-
 substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
170
-
 substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
220
-
 substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
243
-
 substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
328
-
 substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
340
-
 substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 7.8, 30°C Serratia marcescens
370
-
 substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
382
-
 substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
408
-
 substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
457
-
 substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
519
-
 substrate t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
570
-
 substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Escherichia coli
645
-
 substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
663
-
 substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
908
-
 substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
1230
-
 substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
1300
-
 substrate t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
1310
-
 substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
1330
-
 substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
1430
-
 substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
1810
-
 substrate benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
1850
-
 substrate t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
2230
-
 substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
2380
-
 substrate t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
2470
-
 substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3000
-
 substrate t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3055
-
 substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3130
-
 substrate benzoyl-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3275
-
 substrate t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3440
-
 substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3500
-
 substrate benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis
3500
-
 substrate t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Stenotrophomonas maltophilia
3690
-
 substrate t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin, pH 8.0, 30°C Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli benzoyl-Arg + 7-amino-4-methylcoumarin
-
 ?
benzoyl-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia benzoyl-Arg + 7-amino-4-methylcoumarin
-
 ?
benzoyl-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis benzoyl-Arg + 7-amino-4-methylcoumarin
-
 ?
benzoyl-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens benzoyl-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Ala-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Arg-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Glu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Stenotrophomonas maltophilia t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Rhodococcus erythropolis t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
t-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Serratia marcescens t-butyloxycarbonyl-Val-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Escherichia coli
20
-
-
 Rhodococcus erythropolis
20
-
-
 Serratia marcescens
30
-
-
 Stenotrophomonas maltophilia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 30 min, 25% loss of activity Stenotrophomonas maltophilia
40
-
 30 min, 75% loss of activiy Stenotrophomonas maltophilia
50
-
 15 min, complete loss of activity Stenotrophomonas maltophilia