Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leishmania major |
Crystallization (Comment) | Organism |
---|---|
in complex with the inhibitor antipain and as mercury derivative, to 1.65 and 2.0 A resolution, respectively. OPB comprises two domains, a catalytic and a propeller domain, linked together by a hinge region. Residue E621 plays a critical role in the S1 binding pocket and, along with F603, is largely responsible for the enzyme substrate specificity in P1. In the S2 binding pocket, Y499 is important for substrate stability. Residue E623 forms an inter-domain hydrogen bond | Leishmania major |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | - |
Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00093 | - |
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
0.0012 | - |
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
0.0031 | - |
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
0.0038 | - |
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
0.0044 | - |
benzoyl-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | Q4QHU7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Leishmania major | benzoyl-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Leishmania major | benzyloxycarbonyl-Gly-L-Arg-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | no substrate: N-benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin | Leishmania major | ? | - |
? | |
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Leishmania major | N-benzyloxycarbonyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Leishmania major | N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Leishmania major | N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
0.011 | - |
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
0.013 | - |
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
0.016 | - |
benzoyl-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
0.02 | - |
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
1.1 | - |
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
3.6 | - |
benzoyl-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
3.6 | - |
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major | |
5.3 | - |
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin | 21°C, pH not specified in the publication | Leishmania major |