Cloned (Comment) | Organism |
---|---|
gene APH, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21 Transetta (DE3) | Bombyx mori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chlorpyrifos | significantly inhibits the activity of enzyme APH both in vitro and in vivo | Bombyx mori | |
malathion | significantly inhibits the activity of enzyme APH both in vitro and in vivo | Bombyx mori | |
phoxim | significantly inhibits the activity of enzyme APH both in vitro and in vivo | Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.82 | - |
N-acetyl-L-Ala-4-nitroanilide | pH 7.5, 25°C, recombinant enzyme | Bombyx mori | |
0.83 | - |
N-acetyl-L-Ala-4-nitroanilide | pH 7.5, 45°C, recombinant enzyme | Bombyx mori |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
basement membrane | - |
Bombyx mori | 5604 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | A0A0X9PFK4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 Transetta (DE3) by nickel affinity chromatography | Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
midgut | - |
Bombyx mori | - |
additional information | enzyme BmAPH is expressed in all tested tissues and developmental stages of the silkworm, immunohistochemistry analysis. BmAPH protein is localized in the basement membranes | Bombyx mori | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-Ala-4-nitroanilide + H2O | - |
Bombyx mori | N-acetyl-L-Ala + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78500, about, sequence calculation, x * 80000, recombinant His-tagged enzyme, SDS-PAGE | Bombyx mori |
More | enzyme peptide mapping, mass spectrometric analysis, overview | Bombyx mori |
Synonyms | Comment | Organism |
---|---|---|
acylpeptide hydrolase | - |
Bombyx mori |
APH | - |
Bombyx mori |
BmAPH | - |
Bombyx mori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Bombyx mori |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
the IC50 values of chlorpyrifos, phoxim, and malathion with recombinant enzyme are 1.60, 7.90, and 7.62 mg/l, respectively, pH 7.5, 25°C | Bombyx mori | additional information |
General Information | Comment | Organism |
---|---|---|
additional information | the catalytic triad is composed by catalytic residues Ser566, Asp654, and His686 | Bombyx mori |
physiological function | enzyme BmAPHmay be involved in enhancing silkworm tolerance to organophosphorus (OP) insecticides | Bombyx mori |