Cloned (Comment) | Organism |
---|---|
gene cgkA, recombinant expression of functional CBM-tagged chimeric fusion enzyme cCgkA-cCglA, consisting of the catalytic domains of kappa-carrageenase CgkA and lambda-carrageenase CglA from Pseudoalteromonas carrageenovora fused with a dockerin domain, in Escherichia coli strain BL21(DE3), cloning in Escherichia coli strain DH5alpha | Pseudoalteromonas carrageenovora |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a chimeric fusion protein consisting of the catalytic domains of kappa-carrageenase CgkA and lambda-carrageenase CglA from Pseudoalteromonas carrageenovora fused with a dockerin domain from Clostridium cellulovorans. The recombinant cCgkA and cCglA are assembled with scaffoldin miniCbpA from Clostridium cellulovorans via cohesin and dockerin interaction. Carbohydrate binding module in scaffoldin is used as a tag | Pseudoalteromonas carrageenovora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, catalytic domain of kappa-carrageenase CgkA, SDS-PAGE | Pseudoalteromonas carrageenovora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas carrageenovora | P43478 | gene cgkA | - |
Purification (Comment) | Organism |
---|---|
recombinant CBM-tagged chimeric fusion enzyme cCgkA-cCglA by cellulose affinity purification using cellulose as a support or by nickel affinity chromatography | Pseudoalteromonas carrageenovora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageen + H2O | - |
Pseudoalteromonas carrageenovora | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, catalytic domain of kappa-carrageenase CgkA, SDS-PAGE | Pseudoalteromonas carrageenovora |
Synonyms | Comment | Organism |
---|---|---|
CgkA | - |
Pseudoalteromonas carrageenovora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Pseudoalteromonas carrageenovora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudoalteromonas carrageenovora |