General Stability | Organism |
---|---|
repeated freezing and thawing drastically reduces activity | Microbulbifer elongatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AlCl3 | 1 mM, 39.7% inhibition | Microbulbifer elongatus | |
BaCl2 | 1 mM, 26.8% inhibition | Microbulbifer elongatus | |
CaCl2 | 1 mM, 50.7% inhibition | Microbulbifer elongatus | |
FeCl3 | 1 mM, 36.5% inhibition | Microbulbifer elongatus | |
HgCl2 | 1 mM, 40.9% inhibition | Microbulbifer elongatus | |
KCl | 1 mM, 38% inhibition | Microbulbifer elongatus | |
MgCl2 | 1 mM, 52.9% inhibition | Microbulbifer elongatus | |
MnCl2 | 1 mM, 36% inhibition | Microbulbifer elongatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value for kappa-carrageenan is 6.66 mg/ml | Microbulbifer elongatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Microbulbifer elongatus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
128000 | - |
gel filtrration | Microbulbifer elongatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbulbifer elongatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Microbulbifer elongatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
426.2 | - |
- |
Microbulbifer elongatus |
Storage Stability | Organism |
---|---|
20°C, 5 days, 40% loss of activity | Microbulbifer elongatus |
frozen, stable for 15 days | Microbulbifer elongatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-carrageenan + H2O | - |
Microbulbifer elongatus | ? | - |
? | |
additional information | isolated kappa-carrageenase can successfully generate protoplasts of Kappaphycus alvarezii. No activity with lambda-carrageenan, ioro-carrageenan and LMP agarose | Microbulbifer elongatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 128000, SDS-PAGE | Microbulbifer elongatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Microbulbifer elongatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
40% loss of activity within 5 days, complete loss of activity within 12 days | Microbulbifer elongatus |
37 | - |
5 days, 50% loss of activity | Microbulbifer elongatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
and a second optimum at pH 7.7 | Microbulbifer elongatus |
7.7 | - |
and a second optimum at pH 5.6 | Microbulbifer elongatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 9 | pH 4.8: about 70% of maximal activity, pH 9.0: about 60% of maximal activity | Microbulbifer elongatus |