Application | Comment | Organism |
---|---|---|
food industry | production of fuel ethanol and ultra-high fructose syrup | Papiliotrema aurea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C | Papiliotrema aurea | |
Ag+ | decreased activity at concentrations of 1.0 mM | Papiliotrema aurea | |
EDTA | strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C | Papiliotrema aurea | |
Hg2+ | decreased activity at concentrations of 1.0 mM | Papiliotrema aurea | |
inulin | - |
Papiliotrema aurea | |
iodoacetic acid | strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C | Papiliotrema aurea | |
Mg2+ | decreased activity at concentrations of 1.0 mM | Papiliotrema aurea | |
phenylmethanesulfonyl fluoride | strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C | Papiliotrema aurea | |
SDS | final concentration of 10.0 mM | Papiliotrema aurea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 20.06 mg/ml for inulin | Papiliotrema aurea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | fermented broth | Papiliotrema aurea | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activating at concentrations of 1.0 mM | Papiliotrema aurea | |
Cu2+ | activating at concentrations of 1.0 mM | Papiliotrema aurea | |
Fe2+ | activating at concentrations of 1.0 mM | Papiliotrema aurea | |
K+ | activating at concentrations of 1.0 mM | Papiliotrema aurea | |
Na+ | activating at concentrations of 1.0 mM | Papiliotrema aurea | |
Zn2+ | activating | Papiliotrema aurea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
after purification, analyzed by SDS-PAGE | Papiliotrema aurea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papiliotrema aurea | - |
marine yeast, isolated from sediment at China South See | - |
Papiliotrema aurea G7a | - |
marine yeast, isolated from sediment at China South See | - |
Purification (Comment) | Organism |
---|---|
7.2-fold purification, analyzed by SDS-PAGE, 2.44-fold increase of specific inulinase activity with 22.4% yield after DEAE sepharose fast flow anion exchange chromatography | Papiliotrema aurea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
reaction mixture consists of 0.1 ml purified inulinase, 0.9 ml acetate buffer, 20 g/l inulin; incubation at 50°C for 10 min; reaction inactivated at 100°C for 10 min | Papiliotrema aurea |
Storage Stability | Organism |
---|---|
65°C, 2 h, 86% residual activity after treatment at 65°C, pH below 4.0 and above 6.5 results in rapid decrease in enzyme activity | Papiliotrema aurea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin + H2O | - |
Papiliotrema aurea | D-fructose + D-glucose + fructooligosaccharides | - |
? | |
inulin + H2O | - |
Papiliotrema aurea G7a | D-fructose + D-glucose + fructooligosaccharides | - |
? | |
inulin + H2O | - |
Papiliotrema aurea | D-fructose + fructooligosaccharides | crude inulinase | ? | |
inulin + H2O | - |
Papiliotrema aurea G7a | D-fructose + fructooligosaccharides | crude inulinase | ? |
Synonyms | Comment | Organism |
---|---|---|
exoinulinase | - |
Papiliotrema aurea |
inulinase | - |
Papiliotrema aurea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Papiliotrema aurea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 70 | - |
Papiliotrema aurea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
above rapidly inactivating, after treatment at 65°C for 2 h still 86.0% residual activity, after treatment of 70°C for 2 h still 60.0% residual activity | Papiliotrema aurea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Papiliotrema aurea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | above and below rapid decrease of enzyme activity | Papiliotrema aurea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | rapid decrease of residual activity above and below, after treatment at pH 6.0 and 4°C for 2 h still 96.0% residual activity | Papiliotrema aurea |