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1,1,1-kestopentaose + H2O
?
-
18% activity compared to levan
-
-
?
1,1,1-kestopentaose + H2O
beta-D-fructose + ?
-
-
-
-
?
1,1,1-kestose + H2O
beta-D-fructose + sucrose
1,1-kestose + H2O
beta-D-fructose + sucrose
1,1-kestotetraose + H2O
?
1,1-nystose + H2O
beta-D-fructose + sucrose
1-kestose + H2O
beta D-fructofuranose + sucrose
1-kestose + H2O
beta-D-fructose + sucrose
1-kestotriose + H2O
beta-D-fructose + sucrose
-
-
-
-
?
1-nystose + H2O
?
-
-
-
-
?
1F,6G-di-beta-D-fructofuranosylsucrose + H2O
?
1 and 6G-kestotetraose, 57-69% activity compared to neokestose
-
-
?
1F-fructofuranosylnystose + H2O
?
6,6-kestotetraose + H2O
?
6-kestose + H2O
?
-
2% activity compared to 1-kestose
-
-
?
6-kestose + H2O
beta-D-fructose + sucrose
6G(1-beta-D-fructofuranosyl)2sucrose + H2O
?
i.e. 1,6G-kestotetraose, 14-15% activity compared to neokestose
-
-
?
6G,6-kestotetraose + H2O
D-fructose + neokestose
-
100%, chicorium inulin 11.9%, Aerobacter levan 8.5%, sucrose 0% rate of hydrolysis
-
?
Actilight + H2O
?
-
highest activity, Actilight is a commercial mixture composed of 4.8% (w/v) of free sugars (sucrose, glucose and fructose), 35.1% w/v of 1-kestose, 53% w/v of 1-nystose and 7.1% w/v of 1-fructofuranosyl-nystose
-
-
?
Agave tequilana fructan + H2O
?
-
-
-
-
?
burdock root sugar extract + H2O
fructose + sucrose + ?
-
-
-
-
?
chicory root inulin + H2O
beta-D-fructose + fructooligosaccharides
-
-
-
-
?
fructan + H2O
?
-
-
-
-
?
fructan + H2O
beta-D-fructose + ?
fructans + H2O
D-fructose
fructofuranosylnystose + H2O
?
-
high activity
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
inulin + H2O
1-kestose + ?
-
-
-
-
?
inulin + H2O
beta-D-fructose + ?
inulin + H2O
beta-D-fructose + sucrose + ?
inulin + H2O
beta-D-Fruf-(2-1)-beta-D-Fruf + ?
-
-
-
-
?
inulin + H2O
D-fructose + ?
inulin + H2O
D-fructose + D-glucose + fructooligosaccharides
inulin + H2O
D-fructose + fructooligosaccharides
inulin + H2O
D-glucose + D-fructose + ?
inulin + H2O
oligofructosides
inulin + H2O
sucrose + ?
-
-
-
-
?
inulin-type fructans (beta-2,1) + H2O
D-fructose
-
poorly substrate
-
-
?
inulobiose + H2O
?
-
12% activity compared to 1-kestose
-
-
?
inulobiose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulin is an intermediate product
-
-
?
inuloheptaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulohexaose, inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulohexaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulopentaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulotetraose, inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulotetraose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulotriose + H2O
?
-
41% activity compared to 1-kestose
-
-
?
inulotriose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulobiose and inulin are intermediate products
-
-
?
levan + H2O
beta-D-fructofuranose + alpha-D-glucopyranose + ?
-
-
-
?
levan + H2O
beta-D-fructose + ?
levan + H2O
D-fructose + ?
levan-type fructan + H2O
beta-D-fructose + ?
-
-
-
-
?
levan-type fructans (beta-2,6) + H2O
D-fructose
-
best substrate
-
-
?
levanbiose + H2O
beta-D-fructose
-
-
-
-
?
levanohexaose + 6 H2O
6 beta-D-fructofuranose
levanopentaose, levanotetraose, levanotriose, levanobiose and levan are intermediate products
-
-
?
levanopentaose + 4 H2O
5 beta-D-fructofuranose
levanotetraose, levanotriose, levanobiose and levan are intermediate products
-
-
?
levanotetraose + 3 H2O
4 beta-D-fructofuranose
levanotriose, levanobiose and levan are intermediate products
-
-
?
levanotriose + 2 H2O
3 beta-D-fructofuranose
levanobiose and levan are intermediate products
-
-
?
neokestin + H2O
D-fructose
-
-
-
?
neokestose + H2O
beta-D-fructose + ?
neokestose + H2O
beta-D-fructose + sucrose
-
4% activity of isoform w1 and 3% of w2 in comparison to substrate 1-kestose
-
-
?
neokestose + H2O
D-fructose + sucrose
-
-
-
?
phlein + H2O
beta-D-fructose + ?
-
-
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranose
-
-
-
-
?
raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
-
-
-
?
raffinose + H2O
D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
stachyose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranose
-
-
-
-
?
sucrose + H2O
?
0.3% activity compared to 1-kestose
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
3% enzyme activity in comparison to levan
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
sucrose + H2O
beta-D-fructose + alpha-D-glucose
sucrose + H2O
beta-D-fructose + D-glucose
sucrose + H2O
D-fructose + D-glucose
sucrose + H2O
D-glucose + D-fructose
-
-
-
-
?
wheat graminan + H2O
?
recombinant enzyme Wfh-sm3 is able to hydrolyze all components of wheat graminan
-
-
?
wheat graminan + H2O
beta-D-fructose + ?
-
high degree of polymerization
-
-
?
additional information
?
-
1,1,1-kestose + H2O
beta-D-fructose + sucrose
-
45% enzyme activity in comparison to levan
-
-
?
1,1,1-kestose + H2O
beta-D-fructose + sucrose
-
45% enzyme activity in comparison to levan
-
-
?
1,1-kestose + H2O
beta-D-fructose + sucrose
-
39% enzyme activity in comparison to levan
-
-
?
1,1-kestose + H2O
beta-D-fructose + sucrose
-
39% enzyme activity in comparison to levan
-
-
?
1,1-kestotetraose + H2O
?
100% activity
-
-
?
1,1-kestotetraose + H2O
?
-
18% activity compared to levan
-
-
?
1,1-kestotetraose + H2O
?
-
gene product Wfh-sm1 shows 8% activity with 1,1-kestotetraose compared to sucrose
-
-
?
1,1-nystose + H2O
beta-D-fructose + sucrose
-
-
-
-
?
1,1-nystose + H2O
beta-D-fructose + sucrose
-
72% activity of isoform w1 and 83% of w2 in comparison to substrate 1-kestose
-
-
?
1-kestose + H2O
?
-
-
-
?
1-kestose + H2O
?
-
100% activity
-
-
?
1-kestose + H2O
?
-
-
-
?
1-kestose + H2O
?
100% activity
-
-
?
1-kestose + H2O
?
-
-
-
-
?
1-kestose + H2O
beta D-fructofuranose + sucrose
-
-
-
?
1-kestose + H2O
beta D-fructofuranose + sucrose
-
100% activity of both isoforms w1 and w2
-
-
?
1-kestose + H2O
beta-D-fructose + sucrose
low activity
-
-
?
1-kestose + H2O
beta-D-fructose + sucrose
-
-
-
?
1-kestose + H2O
beta-D-fructose + sucrose
-
-
-
?
1-kestose + H2O
beta-D-fructose + sucrose
-
-
-
-
?
1-kestose + H2O
beta-D-fructose + sucrose
-
best substrate
-
-
?
1-kestose + H2O
beta-D-fructose + sucrose
-
12% enzyme activity in comparison to levan
-
-
?
1-kestose + H2O
beta-D-fructose + sucrose
-
12% enzyme activity in comparison to levan
-
-
?
1-kestose + H2O
beta-D-fructose + sucrose
-
-
-
-
?
1-kestotriose + H2O
?
58% activity compared to 1,1-kestotetraose
-
-
?
1-kestotriose + H2O
?
-
7% activity compared to levan
-
-
?
1-kestotriose + H2O
?
-
gene product Wfh-sm1 shows 40% activity with 1-kestotriose compared to sucrose
-
-
?
1F-fructofuranosylnystose + H2O
?
98% activity compared to 1-kestose
-
-
?
1F-fructofuranosylnystose + H2O
?
99% activity compared to 1-kestose
-
-
?
6,6-kestotetraose + H2O
?
-
-
-
?
6,6-kestotetraose + H2O
?
-
-
-
?
6-kestose + H2O
beta-D-fructose + sucrose
low activity
-
-
?
6-kestose + H2O
beta-D-fructose + sucrose
-
-
-
-
?
6-kestose + H2O
beta-D-fructose + sucrose
-
0.7% activity of isoform w1 and 1% of w2 in comparison to substrate 1-kestose
-
-
?
6-kestotriose + H2O
?
best substrate
-
-
?
6-kestotriose + H2O
?
6% activity compared to 1,1-kestotetraose
-
-
?
6-kestotriose + H2O
?
best substrate
-
-
?
6-kestotriose + H2O
?
-
25% activity compared to levan
-
-
?
6-kestotriose + H2O
?
-
gene product Wfh-sm1 shows 38% activity with 6-kestotriose compared to sucrose
-
-
?
6G-kestotriose + H2O
?
lower activity
-
-
?
6G-kestotriose + H2O
?
19% activity compared to 1,1-kestotetraose
-
-
?
6G-kestotriose + H2O
?
lower activity
-
-
?
fructan + H2O
beta-D-fructose + ?
-
-
-
-
?
fructan + H2O
beta-D-fructose + ?
-
-
-
-
?
fructan + H2O
beta-D-fructose + ?
-
-
-
-
?
fructan + H2O
beta-D-fructose + ?
-
-
-
?
fructan + H2O
beta-D-fructose + ?
-
-
-
-
?
fructans + H2O
D-fructose
-
degradation
-
-
?
fructans + H2O
D-fructose
-
degradation
-
-
?
fructans + H2O
D-fructose
-
degradation
-
-
?
fructosylnystose + H2O
?
-
97% activity compared to 1-kestose
-
-
?
fructosylnystose + H2O
?
35-38% activity compared to neokestose
-
-
?
garlic extract + H2O
?
-
60% enzyme activity in comparison to levan
-
-
?
garlic extract + H2O
?
-
60% enzyme activity in comparison to levan
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
no activity with melizitose
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
no activity with melizitose
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
24% activity compared to 1,1-kestotetraose
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
no activity with bacterial levan
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
no activity with bacterial levan
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
6% activity compared to levan
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
inulin + H2O
?
-
42% activity compared to 1-kestose
-
-
?
inulin + H2O
?
24-26.5% activity compared to neokestose
-
-
?
inulin + H2O
?
and hydrolysis of inulin-type fructans
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharides are detected, exoinulinase activity
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharides are detected, exoinulinase activity
-
-
?
inulin + H2O
?
-
high activity
-
-
?
inulin + H2O
?
25% activity compared to 1-kestose
-
-
?
inulin + H2O
?
31% activity compared to 1-kestose
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharide are produced
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharides are detected after the hydrolysis
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharide are produced
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharides are detected, exoinulinase activity
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharides are detected, exoinulinase activity
-
-
?
inulin + H2O
?
-
gene product Wfh-sm1 shows less than 1% activity with inulin compared to sucrose
-
-
?
inulin + H2O
beta-D-fructose + ?
-
-
-
-
?
inulin + H2O
beta-D-fructose + ?
-
-
-
-
?
inulin + H2O
beta-D-fructose + ?
-
-
-
-
?
inulin + H2O
beta-D-fructose + ?
-
-
-
?
inulin + H2O
beta-D-fructose + ?
-
-
-
-
?
inulin + H2O
beta-D-fructose + sucrose + ?
-
9% enzyme activity in comparison to levan
-
-
?
inulin + H2O
beta-D-fructose + sucrose + ?
-
-
-
?
inulin + H2O
D-fructose + ?
-
-
-
-
?
inulin + H2O
D-fructose + ?
-
-
after incubation at 50°C for 1 h
-
?
inulin + H2O
D-fructose + ?
-
-
-
-
?
inulin + H2O
D-fructose + ?
-
root powder extract of Asparagus officinalis. The product mainly consists of fructose and traces of glucose
-
-
?
inulin + H2O
D-fructose + ?
-
root powder extract of Asparagus officinalis. The product mainly consists of fructose and traces of glucose
-
-
?
inulin + H2O
D-fructose + ?
-
-
-
-
?
inulin + H2O
D-fructose + ?
-
-
-
-
?
inulin + H2O
D-fructose + ?
-
-
-
?
inulin + H2O
D-fructose + ?
-
-
-
?
inulin + H2O
D-fructose + ?
-
-
-
?
inulin + H2O
D-fructose + ?
-
-
-
?
inulin + H2O
D-fructose + ?
-
39% activity of isoform w1 and 22% of w2 in comparison to substrate 1-kestose
-
-
?
inulin + H2O
D-fructose + D-glucose + fructooligosaccharides
-
a typical solution product consists of a mixture of fructose (155 g/l), glucose (155 g/l), sucrose (132 g/l) and fructooligosaccharides (50 g/l)
-
-
?
inulin + H2O
D-fructose + D-glucose + fructooligosaccharides
-
-
-
-
?
inulin + H2O
D-fructose + D-glucose + fructooligosaccharides
-
-
-
-
?
inulin + H2O
D-fructose + fructooligosaccharides
-
-
crude inulinase
-
?
inulin + H2O
D-fructose + fructooligosaccharides
-
-
crude inulinase
-
?
inulin + H2O
D-glucose + D-fructose + ?
-
-
-
-
?
inulin + H2O
D-glucose + D-fructose + ?
-
-
-
-
?
inulin + H2O
oligofructosides
-
-
-
-
?
inulin + H2O
oligofructosides
-
-
-
-
?
levan + H2O
?
-
-
-
?
levan + H2O
?
2% activity compared to 1-kestose
-
-
?
levan + H2O
?
-
low to moderate activity
-
-
?
levan + H2O
?
0.9% activity compared to 1-kestose
-
-
?
levan + H2O
?
lower activity
-
-
?
levan + H2O
?
lower activity
-
-
?
levan + H2O
?
-
commercial Erwinia herbicola levan and levan from Leuconostoc mesenteroides DSM 20343-fermented fava bean doughs, combined activity of exoinulinase/levanase
-
-
?
levan + H2O
?
-
gene product Wfh-sm1 shows less than 1% activity with levan compared to sucrose
-
-
?
levan + H2O
beta-D-fructose + ?
-
-
-
-
?
levan + H2O
beta-D-fructose + ?
-
-
-
-
?
levan + H2O
beta-D-fructose + ?
-
-
-
-
?
levan + H2O
beta-D-fructose + ?
-
-
-
-
?
levan + H2O
beta-D-fructose + ?
-
-
-
-
?
levan + H2O
beta-D-fructose + ?
-
100% activity
-
-
?
levan + H2O
beta-D-fructose + ?
-
-
-
-
?
levan + H2O
D-fructose + ?
-
-
-
-
?
levan + H2O
D-fructose + ?
-
-
-
-
?
levan + H2O
D-fructose + ?
-
100% enzyme activity
-
-
?
levan + H2O
D-fructose + ?
-
100% enzyme activity
-
-
?
levan + H2O
D-fructose + ?
0.5% activity compared to 1,1-kestotetraose
-
-
?
levan + H2O
D-fructose + ?
-
-
-
?
levan + H2O
D-fructose + ?
-
-
-
?
levan + H2O
D-fructose + ?
-
-
-
?
levan + H2O
D-fructose + ?
-
4% activity of isoform w1 and 3% of w2 in comparison to substrate 1-kestose
-
-
?
neokestose + H2O
?
-
7% activity compared to 1-kestose
-
-
?
neokestose + H2O
?
preferred substrate
-
-
?
neokestose + H2O
beta-D-fructose + ?
-
-
-
-
?
neokestose + H2O
beta-D-fructose + ?
-
-
-
-
?
neokestose + H2O
beta-D-fructose + ?
-
-
-
-
?
nystose + H2O
?
-
66% activity compared to 1-kestose
-
-
?
nystose + H2O
?
40-50% activity compared to neokestose
-
-
?
nystose + H2O
?
-
-
-
-
?
nystose + H2O
?
52% activity compared to 1-kestose
-
-
?
nystose + H2O
?
58% activity compared to 1-kestose
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
-
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
-
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
hydrolyzed at 69% of the activity with inulin
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
hydrolyzed at 69% of the activity with inulin
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
18% of the activity toward inulin
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
18% of the activity toward inulin
-
-
?
raffinose + H2O
D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
-
-
-
?
raffinose + H2O
D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
-
-
-
?
raffinose + H2O
D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
-
-
-
?
raffinose + H2O
D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
highest activity
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
-
-
-
-
?
sucrose + H2O
beta-D-fructose + alpha-D-glucose
-
100% activity
-
-
?
sucrose + H2O
beta-D-fructose + D-glucose
-
-
-
-
?
sucrose + H2O
beta-D-fructose + D-glucose
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
0.2% activity compared to 1-kestose
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
hydrolyzed at 177.6% of the activity with inulin
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
hydrolyzed at 177.6% of the activity with inulin
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
less than 1%, 2% and 15% activity towards 1 mM, 10 mM and 100 mM sucrose compared to levan
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
?
additional information
?
-
-
no activity towards levan
-
-
?
additional information
?
-
-
the enzyme preferentially hydrolyzes the beta-2,1 linkage of terminal fructosyl residue of fructan
-
-
?
additional information
?
-
fructan 6G&1-exohydrolase (6G&1-FEH) hydrolyzes the terminal beta-2,6 and beta-2,1 fructosyl linkages of fructan, substrate specificity, overview. Levan (from Erwinia herbicola) and sucrose are poor substrates. Hydrolysis of inulin-type fructans
-
-
?
additional information
?
-
fructan 6G&1-exohydrolase (6G&1-FEH) hydrolyzes the terminal beta-2,6 and beta-2,1 fructosyl linkages of fructan, substrate specificity, overview. Levan (from Erwinia herbicola) and sucrose are poor substrates. Hydrolysis of inulin-type fructans
-
-
?
additional information
?
-
fructan 6G&1-exohydrolase (6G&1-FEH) hydrolyzes the terminal beta-2,6 and beta-2,1 fructosyl linkages of fructan, substrate specificty, overview
-
-
?
additional information
?
-
fructan 6G&1-exohydrolase (6G&1-FEH) hydrolyzes the terminal beta-2,6 and beta-2,1 fructosyl linkages of fructan, substrate specificty, overview
-
-
?
additional information
?
-
-
the enzyme catalyses the fructan breakdown in plants
-
-
?
additional information
?
-
-
the enzyme is involved in fructan breakdown
-
-
?
additional information
?
-
-
the enzyme is not able to break the alpha (1-2) linkage present in sucrose
-
-
?
additional information
?
-
no activity towards sucrose
-
-
?
additional information
?
-
-
no activity towards sucrose
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
?
additional information
?
-
inactive with sucrose
-
-
?
additional information
?
-
inactive with sucrose
-
-
?
additional information
?
-
-
inactive with sucrose
-
-
?
additional information
?
-
no hydrolase activity towards sucrose and loliose
-
-
?
additional information
?
-
-
no hydrolase activity towards sucrose and loliose
-
-
?
additional information
?
-
substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose
-
-
?
additional information
?
-
-
substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose
-
-
?
additional information
?
-
substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose
-
-
?
additional information
?
-
-
exo-beta-fructosidases, including exoinulinases (EC 3.2.1.80), can nonspecifically hydrolyze both beta-(2->1) and beta-(2->6)-linkages from the non-reducing end of fructooligosaccharides and fructans. In order to develop a specific analysis for levan in food matrices, a Paenibacillus amylolyticus endolevanase (EC 3.2.1.65) is combined with exoinulinase for levan hydrolysis achieving about 80% hydrolysis yield (19.1% for exoinulinase alone), method evaluation, and modelling. The levanase preparation exhibits an activity of 1116 U/ml towards levan and no activity towards inulin as determined by the reducing value method
-
-
?
additional information
?
-
-
the recombinant enzyme completely degrades fructans that are composed mainly of beta(2,6)-linked and linear fructans (levan) with a high degree of polymerization. The enzyme has significant activity against beta(2,1)-linked fructans, but considerably less than against beta(2,6)-linked fructans. The enzyme also has weak invertase activity
-
-
?
additional information
?
-
-
not: melizitose, dextran, pseudonigeran
-
-
?
additional information
?
-
-
the enzyme shows invertase activity releasing fructose and glucose from sucrose, EC 3.2.1.26
-
-
?
additional information
?
-
-
the enzyme might play a role as a beta-(2,1)-trimmer throughout the period of active graminan
-
-
?
additional information
?
-
-
no activity towards sucrose and high degree of polymerization-inulin
-
-
?
additional information
?
-
-
no activity towards sucrose, 1-kestose, nystose, inulin
-
-
?
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1,10-phenanthroline
-
strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C
2,5 dideoxy-2,5-imino-D-mannitol
-
Al3+
at 55ºC, pH 4.5, 5 mM, 89% inhibition
Ba2+
-
more than 50% of residual enzymatic activity at 20 mM
CuSO4
-
1 mM, 47% inhibition
Fe2(SO4)3
-
1 mM, 38% inhibition
iodoacetate
-
30 min at 30ºC, 10 mM, 87% inhibition; 30 min at 30ºC, 1 mM, 38% inhibition; 30 min at 30ºC, 5 mM, 57% inhibition
iodoacetic acid
-
strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C
N-bromosuccinimide
1.0 mM, pH 5.0, at 30ºC, 100% inhibition
phenylmethanesulfonyl fluoride
-
strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C
SDS
-
final concentration of 10.0 mM
Sodium arsenate
-
30 min at 30ºC, 10 mM, 50% inhibition; 30 min at 30ºC, 1 mM, 20% inhibition; 30 min at 30ºC, 5 mM, 38% inhibition
sodium arsenite
-
30 min at 30ºC, 10 mM, 58% inhibition; 30 min at 30ºC, 1 mM, 28% inhibition; 30 min at 30ºC, 5 mM, 41% inhibition
Ag+
-
potent inhibitor below 1 mM
Ag+
-
more than 50% of residual enzymatic activity at 20 mM
Ag+
-
decreased activity at concentrations of 1.0 mM
Ag+
1.0 mM, pH 5.0, at 30ºC, 100% inhibition
Co2+
-
30 min at 30ºC, 10 mM, 70% inhibition; 30 min at 30ºC, 1 mM, 17% inhibition; 30 min at 30ºC, 5 mM, 39% inhibition
Cu2+
-
30 min at 30ºC, 10 mM, 100% inhibition; 30 min at 30ºC, 1 mM, 23% inhibition; 30 min at 30ºC, 5 mM, 87% inhibition
Cu2+
at 55ºC, pH 4.5, 5 mM, 20% inhibition
Cu2+
-
less than 50% of residual enzymatic activity at 20 mM
Cu2+
1.0 mM, pH 5.0, at 30ºC, 26% inhibition
D-glucose
-
competitive inhibitor
D-glucose
at 55ºC, pH 4.5, concentration higher than 2%
EDTA
-
30 min at 30ºC, 10 mM, 10% inhibition; 30 min at 30ºC, 5 mM, 9% inhibition
EDTA
-
1 mM, 63% of inhibition
EDTA
-
1 mM, 50% inhibition
EDTA
-
strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C
EDTA
1.0 mM, pH 5.0, at 30ºC, 12% inhibition
Fe2+
-
more than 50% of residual enzymatic activity at 20 mM
Fe3+
-
30 min at 30ºC, 10 mM, 86% inhibition; 30 min at 30ºC, 1 mM, 19% inhibition; 30 min at 30ºC, 5 mM, 60% inhibition
Fe3+
at 55ºC, pH 4.5, 5 mM, 45% inhibition
Fe3+
-
more than 50% of residual enzymatic activity at 20 mM
Fe3+
1.0 mM, pH 5.0, at 30ºC, 62% inhibition
Hg2+
-
30 min at 30ºC, 10 mM, 85% inhibition; 30 min at 30ºC, 1 mM, 26% inhibition; 30 min at 30ºC, 5 mM, 57% inhibition
Hg2+
-
1 mM, 100% of inhibition
Hg2+
at 55ºC, pH 4.5, 5 mM, 91% inhibition
Hg2+
-
potent inhibitor below 1mM
Hg2+
-
less than 50% of residual enzymatic activity at 20 mM
Hg2+
-
decreased activity at concentrations of 1.0 mM
Hg2+
1.0 mM, pH 5.0, at 30ºC, 100% inhibition
Mg2+
at 55ºC, pH 4.5, 5 mM, 41% inhibition
Mg2+
-
decreased activity at concentrations of 1.0 mM
Mn2+
-
30 min at 30ºC, 10 mM, 20% inhibition
Mn2+
-
less than 50% of residual enzymatic activity at 20 mM
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
1.0 mM, pH 5.0, at 30ºC, 65% inhibition
p-chloromercuribenzoate
-
presence of cysteine protects against inactivation but no recovery
sucrose
-
-
sucrose
63% inhibition by 10 mM sucrose, wild-type enzyme. Mutant enzymes W82L and S101L are not inhibited by sucrose. W82 and S101 are important for binding sucrose as inhibitor
sucrose
13% inhibition of isoform 1-FEH I at 10 mM and 49% inhibition at 100 mM sucrose; 21% inhibition of isoform 1-FEH II at 10 mM and 65% at 100 mM sucrose
sucrose
90% inhibition at 20 mM
sucrose
70-90% inhibition at 10-40 mM
sucrose
-
the inhibitory effect of sucrose is 4% at 10 mM sucrose and 23% at 100 mM sucrose
Zn2+
-
30 min at 30ºC, 10 mM, 66% inhibition; 30 min at 30ºC, 1 mM, 22% inhibition; 30 min at 30ºC, 5 mM, 39% inhibition
Zn2+
at 55ºC, pH 4.5, 5 mM, 8% inhibition
Zn2+
-
less than 50% of residual enzymatic activity at 20 mM
additional information
no inhibited by EDTA and 2-mercaptoethanol
-
additional information
-
fructanohydrolase biosynthesis in the basal medium is reduced drastically with the addition of actinomycin D
-
additional information
-
glycosylation of 1-FEH IIa affected overall enzyme activity
-
additional information
glycosylation of 1-FEH IIa affected overall enzyme activity
-
additional information
glucose and fructose (20 mM) do not affect 1-FEHa activity
-
additional information
-
glucose and fructose (20 mM) do not affect 1-FEHa activity
-
additional information
-
not inhibited by sucrose
-
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Norman, J.M.; Bunny, K.L.; Giffard, P.M.
Characterization of levJ, a sucrase/fructanase-encoding gene from Actinomyces naeslundii T14V, and comparison of its product with other sucrose-cleaving enzymes
Gene
152
93-98
1995
Actinomyces naeslundii, Actinomyces naeslundii T14V
brenda
Burne, R.A.; Schilling, K.; Bowen, W.H.; Yasbin, R.E.
Expression, purification, and characterization of an exo-beta-D-fructosidase of Streptococcus mutans
J. Bacteriol.
169
4507-4517
1987
Streptococcus mutans, Streptococcus mutans GS-5
brenda
Takahashi, N.; Mizuno, F.; Takamori, K.
Purification and preliminary characterization of exo-beta-D-fructosidase in Streptococcus salivarius KTA-19
Infect. Immun.
47
271-276
1985
Streptococcus salivarius
brenda
Frehner, M.; Keller, F.; Wiemken, A.
Localisation of fructan metabolism in the vacuoles isolated from protoplasts of jerusalem artichoke tubers (Helianthus tuberosus L.)
J. Plant Physiol.
116
197-208
1984
Helianthus tuberosus
brenda
Igarashi, T.; Yakamoto, A.; Goto, N.
Characterization of an exo-beta-D-fructosidase from Streptococcus mutants ingbritt
Microbiol. Immunol.
36
643-647
1992
Streptococcus mutans
brenda
Burne, R.A.; Penders, J.E.C.
Characterization of the Streptococcus mutans GS-5 fruA gene encoding exo-beta-D-fructosidase
Infect. Immun.
60
4621-4632
1992
Streptococcus mutans, Streptococcus mutans GS-5
brenda
Henson, C.A.; Livingston III, D.P.
Purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes beta-2,6-fructans
Plant Physiol.
110
639-644
1996
Avena sativa
brenda
Henson, C.A.; Livingston III, D.P.
Characterization of a fructan exohydrolase purified from barley stems that hydrolyzes multiple fructofuranosidic linkages
Plant Physiol. Biochem.
36
715-720
1998
Hordeum vulgare
-
brenda
De Roover, J.; Van Laere, A.; De Winter, M.; Timmermanns, J.W.; Van den Ende, W.
Purification and properties of a second fructan exohydrolase from the roots of Chicorium intybus
Physiol. Plant.
106
28-34
1999
Cichorium intybus
-
brenda
Verhaest, M.; Van den Ende, W.; Yoshida, M.; Le Roy, K.; Peeraer, Y.; Sansen, S.; De Ranter, C.J.; Van Laere, A.; Rabijns, A.
Crystallization and preliminary X-ray diffraction study of fructan 1-exohydrolase IIa from Cichorium intybus
Acta Crystallogr. Sect. D
60
553-554
2004
Cichorium intybus
brenda
Gill, P.K.; Manhas, R.K.; Singh, J.; Singh, P.
Purification and characterization of an exoinulinase from Aspergillus fumigatus
Appl. Biochem. Biotechnol.
117
19-32
2004
Aspergillus fumigatus
brenda
Kulminskaya, A.A.; Arand, M.; Eneyskaya, E.V.; Ivanen, D.R.; Shabalin, K.A.; Shishlyannikov, S.M.; Saveliev, A.N.; Korneeva, O.S.; Neustroev, K.N.
Biochemical characterization of Aspergillus awamori exoinulinase: substrate binding characteristics and regioselectivity of hydrolysis
Biochim. Biophys. Acta
1650
22-29
2003
Aspergillus awamori (Q96TU3), Aspergillus awamori
brenda
Moriyama, S.; Akimoto, H.; Suetsugu, N.; Kawasaki, S.; Nakamura, T.; Ohta, K.
Purification and properties of an extracellular exoinulinase from Penicillium sp. strain TN-88 and sequence analysis of the encoding gene
Biosci. Biotechnol. Biochem.
66
1887-1896
2002
Penicillium sp. (Q8J0G1), Penicillium sp., Penicillium sp. TN-88 (Q8J0G1)
brenda
Jing, W.; Zhengyu, J.; Bo, J.; Xueming, X.
Separation and identification of exo- and endoinulinase from Aspergillus ficuum
Curr. Microbiol.
47
109-112
2003
Aspergillus ficuum, Aspergillus ficuum JNSP5-06
brenda
Hamdy, H.S.
Purification and some important characters of extracellular inulinase of Alternaria alternata (Fr.) Keissler
Indian J. Exp. Biol.
40
1393-1398
2002
Alternaria alternata
brenda
Michiels, A.; Van Laere, A.; Van den Ende, W.; Tucker, M.
Expression analysis of a chicory fructan 1-exohydrolase gene reveals complex regulation by cold
J. Exp. Bot.
55
1325-1333
2004
Cichorium intybus (Q93X60), Cichorium intybus
brenda
Van den Ende, W.; Michiels, A.; De Roover, J.; Verhaert, P.; Van Laere, A.
Cloning and functional analysis of chicory root fructan1-exohydrolase I (1-FEH I): a vacuolar enzyme derived from a cell-wall invertase ancestor? Mass fingerprint of the 1-FEH I enzyme
Plant J.
24
447-456
2000
Cichorium intybus
brenda
Van den Ende, W.; De Coninck, B.; Clerens, S.; Vergauwen, R.; Van Laere, A.
Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel 'cell-wall invertase-like' specific 6-FEH from sugar beet (Beta vulgaris L.)
Plant J.
36
697-710
2003
Beta vulgaris
brenda
Van den Ende, W.; Michiels, A.; Van Wonterghem, D.; Clerens, S.P.; De Roover, J.; Van Laere, A.J.
Defoliation induces fructan 1-exohydrolase II in witloof chicory roots. Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase II enzymes
Plant Physiol.
126
1186-1195
2001
Cichorium intybus
brenda
Van Den Ende, W.; Clerens, S.; Vergauwen, R.; Van Riet, L.; Van Laere, A.; Yoshida, M.; Kawakami, A.
Fructan 1-exohydrolases. beta-(2,1)-trimmers during graminan biosynthesis in stems of wheat? Purification, characterization, mass mapping, and cloning of two fructan 1-exohydrolase isoforms
Plant Physiol.
131
621-631
2003
Triticum aestivum
brenda
Zhang, L.; Zhao, C.; Zhu, D.; Ohta, Y.; Wang, Y.
Purification and characterization of inulinase from Aspergillus niger AF10 expressed in Pichia pastoris
Protein Expr. Purif.
35
272-275
2004
Aspergillus niger (O74641), Aspergillus niger AF10 (O74641), Aspergillus niger AF10
brenda
Paludan-Muller, C.; Gram, L.; Rattray, F.P.
Purification and characterisation of an extracellular fructan beta-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish
Syst. Appl. Microbiol.
25
13-20
2002
Lactiplantibacillus pentosus, Lactiplantibacillus pentosus B235
brenda
Uzunova, K.; Vassileva, A.; Kambourova, M.; Ivanova, V.; Spasova, D.; Mandeva, R.; Derekova, A.; Tonkova, A.
Production and properties of a bacterial thermostable exo-inulinase
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Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) 11
brenda
Verhaest, M.; Ende, W.V.; Roy, K.L.; De Ranter, C.J.; Laere, A.V.; Rabijns, A.
X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus
Plant J.
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Cichorium intybus (Q93X60), Cichorium intybus
brenda
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Activities of fructan- and sucrose-metabolizing enzymes in wheat stems subjected to water stress during grain filling
Planta
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2004
Triticum aestivum
brenda
Benkeblia, N.; Ueno, K.; Onodera, S.; Shiomi, N.
Variation of fructooligosaccharides and their metabolizing enzymes in onion bulb (Allium cepa L. cv. Tenshin) during long-term storage
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Allium cepa
brenda
Kawakami, A.; Yoshida, M.; Van den Ende, W.
Molecular cloning and functional analysis of a novel 6&1-FEH from wheat (Triticum aestivum L.) preferentially degrading small graminans like bifurcose
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Triticum aestivum
brenda
Van Riet, L.; Nagaraj, V.; Van den Ende, W.; Clerens, S.; Wiemken, A.; van Laere, A.
Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.)
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Triticum aestivum
brenda
Nagem, R.A.; Rojas, A.L.; Golubev, A.M.; Korneeva, O.S.; Eneyskaya, E.V.; Kulminskaya, A.A.; Neustroev, K.N.; Polikarpov, I.
Crystal structure of exo-inulinase from Aspergillus awamori: the enzyme fold and structural determinants of substrate recognition
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Aspergillus awamori, Aspergillus awamori EI
brenda
De Coninck, B.; Le Roy, K.; Francis, I.; Clerens, S.; Vergauwen, R.; Halliday, A.M.; Smith, S.M.; Van Laere, A.; van den Ende, W.
Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities
Plant Cell Environ.
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brenda
Portes, M.T.; Carvalho, M.A.
Spatial distribution of fructans and fructan metabolizing enzymes in rhizophores of Vernonia herbacea (Vell.) Rusby (Asteraceae) in different developmental phases
Plant Sci.
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Vernonia herbacea, Vernonia herbacea (A9JIF3)
brenda
Van den Ende, W.; De Coninck, B.; Van Laere, A.
Plant fructan exohydrolases: a role in signaling and defense?
Trends Plant Sci.
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Arabidopsis thaliana, Beta vulgaris, Cichorium intybus
brenda
Singh, R.S.; Sooch, B.S.; Puri, M.
Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1
Biores. Technol.
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Kluyveromyces marxianus, Kluyveromyces marxianus Ys-1
brenda
Singh, R.S.; Bhermi, H.K.
Production of extracellular exoinulinase from Kluyveromyces marxianus YS-1 using root tubers of Asparagus officinalis
Biores. Technol.
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Kluyveromyces marxianus, Kluyveromyces marxianus Ys-1
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Zhang, W.; Ge, X.
Improvement of fructanohydrolase production in Aspergillus niger SL-09 by sucrose ester
Food Technol. Biotechnol.
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Aspergillus niger, Aspergillus niger SL-09
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brenda
Santos, A.M.; Maugeri, F.
Synthesis of fructooligosaccharides from sucrose using inulinase from Kluyveromyces marxianus
Food Technol. Biotechnol.
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2007
Kluyveromyces marxianus
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brenda
Moriyama, S.; Muguruma, M.; Ohta, K.
Quantitative expression analysis of inulinase gene cluster of Penicillium sp. strain TN-88
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Penicillium sp. (Q8J0G1), Penicillium sp., Penicillium sp. TN-88 (Q8J0G1)
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Moriyama, S.; Ohta, K.
Functional characterization and evolutionary implication of the internal 157-amino-acid sequence of an exoinulinase from Penicillium sp. strain TN-88
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Penicillium sp., Penicillium sp. TN-88
brenda
Lothier, J.; Lasseur, B.; Le Roy, K.; Van Laere, A.; Prudhomme, M.P.; Barre, P.; Van den Ende, W.; Morvan-Bertrand, A.
Cloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilization
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Lolium perenne (Q64GB3), Lolium perenne
brenda
Narai-Kanayama, A.; Tokita, N.; Aso, K.
Dependence of fructooligosaccharide content on activity of fructooligosaccharide-metabolizing enzymes in yacon (Smallanthus sonchifolius) tuberous roots during storage
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Smallanthus sonchifolius
brenda
Gong, F.; Sheng, J.; Chi, Z.; Li, J.
Inulinase production by a marine yeast Pichia guilliermondii and inulin hydrolysis by the crude inulinase
J. Ind. Microbiol. Biotechnol.
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Meyerozyma guilliermondii
brenda
Singh, R.S.; Dhaliwal, R.; Puri, M.
Partial purification and characterization of exoinulinase from Kluyveromyces marxianus YS-1 for preparation of high-fructose syrup
J. Microbiol. Biotechnol.
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Kluyveromyces marxianus, Kluyveromyces marxianus Ys-1
brenda
Gao, L.; Chi, Z.; Sheng, J.; Wang, L.; Li, J.; Gong, F.
Inulinase-producing marine yeasts: evaluation of their diversity and inulin hydrolysis by their crude enzymes
Microb. Ecol.
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Debaryomyces hansenii, Meyerozyma guilliermondii, Papiliotrema aurea, Debaryomyces hansenii G7a1, Papiliotrema aurea G7a, Meyerozyma guilliermondii OUC1
brenda
Pessoni, R.A.; Braga, M.R.; Figueiredo-Ribeiro, R.d.e.C.
Purification and properties of exo-inulinases from Penicillium janczewskii growing on distinct carbon sources
Mycologia
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brenda
Verhaest, M.; Lammens, W.; Le Roy, K.; De Ranter, C.J.; Van Laere, A.; Rabijns, A.; Van den Ende, W.
Insights into the fine architecture of the active site of chicory fructan 1-exohydrolase: 1-kestose as substrate vs sucrose as inhibitor
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brenda
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N-glycosylation affects substrate specificity of chicory fructan 1-exohydrolase: evidence for the presence of an inulin binding cleft
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Cichorium intybus, Cichorium intybus (Q93X60)
brenda
Le Roy, K.; Lammens, W.; Van Laere, A.; Van den Ende, W.
Influencing the binding configuration of sucrose in the active sites of chicory fructan 1-exohydrolase and sugar beet fructan 6-exohydrolase
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Unraveling the difference between invertases and fructan exohydrolases: a single amino acid (Asp-239) substitution transforms Arabidopsis cell wall invertase1 into a fructan 1-exohydrolase
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Arabidopsis thaliana (Q67XZ3)
brenda
Sheng, J.; Chi, Z.; Gong, F.; Li, J.
Purification and Characterization of Extracellular Inulinase from a Marine Yeast Cryptococcus aureus G7a and Inulin Hydrolysis by the Purified Inulinase
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Papiliotrema aurea, Papiliotrema aurea G7a
brenda
Mutanda, T.; Wilhelmi, B.; Whiteley, C.G.
Controlled production of fructose by an exoinulinase from Aspergillus ficuum
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Aspergillus ficuum
brenda
Zhang, J.; Dell, B.; Conocono, E.; Waters, I.; Setter, T.; Appels, R.
Water deficits in wheat: Fructan exohydrolase (1-FEH) mRNA expression and relationship to soluble carbohydrate concentrations in two varieties
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Triticum aestivum
brenda
Martel, C.M.; Parker, J.E.; Jackson, C.J.; Warrilow, A.G.; Rolley, N.; Greig, C.; Morris, S.M.; Donnison, I.S.; Kelly, D.E.; Kelly, S.L.
Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol
Biores. Technol.
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Bacillus subtilis (P05656), Bacillus subtilis
brenda
Arrizon, J.; Morel, S.; Gschaedler, A.; Monsan, P.
Purification and substrate specificities of a fructanase from Kluyveromyces marxianus isolated from the fermentation process of Mezcal
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Kluyveromyces marxianus
brenda
Ueno, K.; Ishiguro, Y.; Yoshida, M.; Onodera, S.; Shiomi, N.
Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappa L.)
Chem. Cent. J.
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Arctium lappa
brenda
Tamura, K.I.; Sanada, Y.; Tase, K.; Komatsu, T.; Yoshida, M.
Pp6-FEH1 encodes an enzyme for degradation of highly polymerized levan and is transcriptionally induced by defoliation in timothy (Phleum pratense L.)
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Phleum pratense
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Kawakami, A.; Yoshida, M.
Graminan breakdown by fructan exohydrolase induced in winter wheat inoculated with snow mold
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Triticum aestivum
brenda
Martinez, D.; Cutino-Avila, B.; Perez, E.R.; Menendez, C.; Hernandez, L.; Del Monte-Martinez, A.
A thermostable exo-beta-fructosidase immobilised through rational design
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Thermotoga maritima
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Lothier, J.; Van Laere, A.; Prudhomme, M.P.; Van den Ende, W.; Morvan-Bertrand, A.
Cloning and characterization of a novel fructan 6-exohydrolase strongly inhibited by sucrose in Lolium perenne
Planta
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2014
Lolium perenne (D0PQE8), Lolium perenne, Lolium perenne Bravo (D0PQE8)
brenda
Shi, Q.; Hou, Y.; Xu, Y.; Moerkeberg Krogh, K.B.R.; Tenkanen, M.
Enzymatic analysis of levan produced by lactic acid bacteria in fermented doughs
Carbohydr. Polym.
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2019
Paenibacillus amylolyticus
brenda
Gasperl, A.; Morvan-Bertrand, A.; Prudhomme, M.P.; van der Graaff, E.; Roitsch, T.
A simple and fast kinetic assay for the determination of fructan exohydrolase activity in perennial ryegrass (Lolium perenne L.)
Front. Plant Sci.
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Lolium perenne
brenda
Xu, H.; Liang, M.; Xu, L.; Li, H.; Zhang, X.; Kang, J.; Zhao, Q.; Zhao, H.
Cloning and functional characterization of two abiotic stress-responsive Jerusalem artichoke (Helianthus tuberosus) fructan 1-exohydrolases (1-FEHs)
Plant Mol. Biol.
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2015
Helianthus tuberosus (A0A0D5A4E4), Helianthus tuberosus (A0A0D5A4L3), Helianthus tuberosus
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Zhan, W.; Jin, L.; Jiao, J.; Zhang, X.; Zhang, Y.; Zhao, H.; Liang, M.
Expression and purification of plant fructan exohydrolases and their potential applications in fructose production
Int. J. Biol. Macromol.
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2018
Helianthus tuberosus
brenda
Ueno, K.; Sonoda, T.; Yoshida, M.; Shiomi, N.; Onodera, S.
Purification, characterization, and functional analysis of a novel 6G&1-FEH mainly hydrolyzing neokestose from Asparagus
J. Exp. Bot.
69
4295-4308
2018
Asparagus officinalis (A0A2Z6G7P5), Asparagus officinalis (A0A2Z6G7T9)
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Meguro-Maoka, A.; Yoshida, M.
Analysis of seasonal expression levels of wheat fructan exohydrolase (FEH) genes regulating fructan metabolism involved in wintering ability
J. Plant Physiol.
191
54-62
2016
Triticum aestivum (L0N593), Triticum aestivum (Q3MV21), Triticum aestivum
brenda