Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.193 extracted from

  • Liu, C.; Zuo, K.; Yu, H.; Sun, C.; Zhang, T.; Xu, L.; Jin, Y.; Im, W.; Jin, F.
    Preparation of minor ginsenosides C-Mx and C-K from notoginseng leaf ginsenosides by a special ginsenosidase type-I (2015), Process Biochem., 50, 2158-2167 .
No PubMed abstract available
Search for more literature for 3.2.1.193

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Vmax is 79.6 mM/h for Rb1, 45.6 mM/h for Rb2, 1.62 mM/h for Rb3 to Rd, 10.8 mM/h for Rb3 to C-Mx1, and 6.16 mM/h for Rc Aspergillus niger
5.46
-
 ginsenoside Rc pH 5.0, 45°C Aspergillus niger
15.1
-
 ginsenoside Rb3 pH 5.0, 45°C, formation of compound Mx-1 Aspergillus niger
16.6
-
 ginsenoside Rb1 pH 5.0, 45°C Aspergillus niger
16.6
-
 ginsenoside Rb3 pH 5.0, 45°C, formation of ginsenoside Rd Aspergillus niger
20.4
-
 ginsenoside Rb2 pH 5.0, 45°C Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
compound Mc + H2O Aspergillus niger
-
 compound K + L-arabinofuranose
-
 ?
compound Mc1 + H2O Aspergillus niger
-
 compound Mc + D-glucose
-
 ?
compound Mx + H2O Aspergillus niger
-
 compound K + D-xylose
-
 ?
compound Mx-1 + H2O Aspergillus niger
-
 compound Mx + D-glucose
-
 ?
compound O + H2O Aspergillus niger
-
 compound Y + D-glucose
-
 ?
compound Y + H2O Aspergillus niger
-
 compound K + L-arabinopyranose
-
 ?
ginsenoside F2 + H2O Aspergillus niger
-
 compound K + D-glucose
-
 ?
ginsenoside Rb2 + H2O Aspergillus niger
-
 compound O + D-glucose
-
 ?
ginsenoside Rb3 + H2O Aspergillus niger
-
 ginsenoside Rd + D-xylose
-
 ?
ginsenoside Rb3 + H2O Aspergillus niger
-
 compound Mx1 + D-glucose
-
 ?
ginsenoside Rb3 + H2O Aspergillus niger g.848
-
 ginsenoside Rd + D-xylose
-
 ?
ginsenoside Rc + H2O Aspergillus niger
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
ginsenoside Rc + H2O Aspergillus niger
-
 compound Mc1 + D-glucose
-
 ?
ginsenoside Rc + H2O Aspergillus niger g.848
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
ginsenoside Rd + H2O Aspergillus niger
-
 ginsenoside F2 + D-glucose
-
 ?
additional information Aspergillus niger substrates are notoginseng leaf ginsenosides ?
-
 ?
additional information Aspergillus niger g.848 substrates are notoginseng leaf ginsenosides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger g.848
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from mycelium by anion exchange chromatography to homogeneity Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
compound Mc + H2O
-
 Aspergillus niger compound K + L-arabinofuranose
-
 ?
compound Mc1 + H2O
-
 Aspergillus niger compound Mc + D-glucose
-
 ?
compound Mx + H2O
-
 Aspergillus niger compound K + D-xylose
-
 ?
compound Mx-1 + H2O
-
 Aspergillus niger compound Mx + D-glucose
-
 ?
compound O + H2O
-
 Aspergillus niger compound Y + D-glucose
-
 ?
compound Y + H2O
-
 Aspergillus niger compound K + L-arabinopyranose
-
 ?
ginsenoside F2 + H2O
-
 Aspergillus niger compound K + D-glucose
-
 ?
ginsenoside Rb1 + H2O
-
 Aspergillus niger ginsenoside Rd + D-glucose
-
 ?
ginsenoside Rb1 + H2O
-
 Aspergillus niger g.848 ginsenoside Rd + D-glucose
-
 ?
ginsenoside Rb2 + H2O
-
 Aspergillus niger compound O + D-glucose
-
 ?
ginsenoside Rb3 + H2O
-
 Aspergillus niger ginsenoside Rd + D-xylose
-
 ?
ginsenoside Rb3 + H2O
-
 Aspergillus niger g.848 ginsenoside Rd + D-xylose
-
 ?
ginsenoside Rb3 + H2O
-
 Aspergillus niger compound Mx1 + D-glucose
-
 ?
ginsenoside Rc + H2O
-
 Aspergillus niger ginsenoside Rd + L-arabinofuranose
-
 ?
ginsenoside Rc + H2O
-
 Aspergillus niger g.848 ginsenoside Rd + L-arabinofuranose
-
 ?
ginsenoside Rc + H2O
-
 Aspergillus niger compound Mc1 + D-glucose
-
 ?
ginsenoside Rd + H2O
-
 Aspergillus niger ginsenoside F2 + D-glucose
-
 ?
additional information substrates are notoginseng leaf ginsenosides Aspergillus niger ?
-
 ?
additional information the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I Aspergillus niger ?
-
 ?
additional information substrates are notoginseng leaf ginsenosides Aspergillus niger g.848 ?
-
 ?
additional information the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I Aspergillus niger g.848 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
ginsenosidase type-I
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus niger